Whey protein concentrate as a functional matrix in chicken pâté: impacts on physicochemical stability and lipid oxidation

Autores

  • Chrystiellen Ayana Aparecida Rodrigues
  • Pollyanna Aparecida Dias
  • Mauro Ramalho Silva
  • Harriman Aley Morais

DOI:

https://doi.org/10.34188/bjaerv9n2-043

Palavras-chave:

poultry products, fat replacers, dairy by-products, binder properties, TBARS analysis

Resumo

Emulsified meat products, such as pâtés, are complex matrices whose stability depends on the interaction between proteins, lipids, and water. This study investigated the effects of using Whey Protein Concentrate (WPC) as a functional binder and fat replacer in chicken pâté. Physicochemical stability was evaluated during storage, focusing on pH levels, instrumental color (L∗, a∗, and b∗), and lipid oxidation via TBARS analysis. The results indicated that WPC significantly influenced the product’s stability. While pH values remained stable within a range of 6.1 to 6.4, ensuring the technological integrity of the meat matrix, the inclusion of WPC led to higher lightness (L∗) and a increase in redness (a∗), typical of poultry emulsions. However, formulations with WPC showed increased susceptibility to lipid oxidation; samples with 25% and 50% fat replacement exhibited a progressive rise in malonaldehyde content during storage. Despite the oxidative challenges, the incorporation of WPC proved to be a viable strategy for enhancing the nutritional profile of chicken pâté without compromising its essential physicochemical properties, provided that antioxidant strategies are considered for long-term storage.

Referências

AEWSIRI, T.; GANESAN, P.; THONGZAI, H. Whey protein–tannic acid conjugate stabilized emulsion-type pork sausages: a focus on lipid oxidation and physicochemical features. Foods, Basel, v. 12, n. 14, p. 2766, 2023. Disponível em: https://www.mdpi.com/2304-8158/12/14/2766. Acesso em: 12 mar. 2026.

AKSAKAL, N. et al. Application of protein concentrate-based gel emulsions for animal fat reduction in meat emulsions: proximate composition, technological, and textural properties. Meat Technology, Belgrado, v. 66, n. 3, p. 260–265, 2025. Disponível em: http://doi.fil.bg.ac.rs/volume.php?pt=journals&issue=meattech-2025-66-3&i=42. Acesso em: 2 mar. 2026.

ALVES, R. de A. et al. Antioxidantes naturais e aceitação sensorial em produtos cárneos: revisão da evidência científica. Revista Ciência, Tecnologia & Ambiente, São Carlos, v. 14, p. e14267, 2024. Disponível em: https://www.revistacta.ufscar.br/index.php/revistacta/article/view/267. Acesso em: 1 abr. 2026.

AMERICAN MEAT SCIENCE ASSOCIATION. Meat color measurement guidelines. Champaign: AMSA, 2012. Disponível em: https://meatscience.org/docs/default-source/publications-resources/hot-topics/2012_12_meat_clr_guide.pdf. Acesso em: 12 mar. 2026.

BRASIL, M. da A. e do A. Instrução Normativa n.o 21, de 31 de julho de 2000. Aprovar osRegulamentos Técnicos de Identidade e- Qualidade -de Patê, de Bacon-ouBarriga Defumada e-de Lombo Suíno. Diário Oficial da União, Seção 1, n. 21, 21, 12–13, 2000. Disponível em: https://pesquisa.in.gov.br/imprensa/jsp/visualiza/index.jsp?data=03/08/2000&jornal=1&pagina=60&totalArquivos=88. Acesso em: 4 dez. 2020.

BRASIL. Métodos oficiais para análise de produtos de origem znimal. Brasília, DF: MAPA, 2022. Disponível em: https://www.gov.br/agricultura/pt-br/assuntos/lfda/legislacao-metodos-da-rede-lfda/poa/metodos_oficiais_para_analise_de_produtos_de_origem_animal-_1a_ed-_2022_assinado.pdf. Acesso em: 7 out. 2024.

CHEN, X. et al. Study on meat color stability of Qinchuan cattle during post-slaughter storage. Food Science and Technology, Campinas, v. 43, p. e101222, 2023. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100454&tlng=en. Acesso em: 6 mar. 2026.

CZARNIECKA-SKUBINA, E. et al. Whey—a valuable technological resource for the production of new functional products with added health-promoting properties. Foods, Basel, v. 14, n. 24, p. 4258, 2025. Disponível em: https://www.mdpi.com/2304-8158/14/24/4258. Acesso em: 27 fev. 2026.

DIAS, P. A. et al. Effect of whey protein concentrate on the physicochemical and oxidative stability of reduced-sodium chicken pâtés. Brazilian Journal of Technology, Curitiba, v. 9, n. 1, p. e85302, 2026. Disponível em: https://ojs.brazilianjournals.com.br/ojs/index.php/BJT/article/view/85302. Acesso em: 3 mar. 2026.

DOMÍNGUEZ, R. et al. Elevating meat products: Unleashing novel gel techniques for enhancing lipid profiles. Meat Science, Amsterdã, v. 204, p. 109277, 2023. Disponível em: https://doi.org/10.1016/j.meatsci.2023.109277. Acesso em: 27 fev. 2026.

EL-MAGOLI, S. B.; LAROLA, S.; HANSEN, P. M. T. Ultrastructure of low-fat ground beef patties with added whey protein concentrate. Food Hydrocolloids, Amsterdâ, v. 9, n. 4, p. 291–306, 1995. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0268005X09802609. Acesso em: 12 mar. 2026.

ESPINOZA-LEANDRO, Y. K.; OLIVERA-MONTENEGRO, L.; PAREDES-CONCEPCIÓN, P. Meat, dairy and vegetable emulsions: recent innovations in the development of functional, healthy and more stable foods. Scientia Agropecuaria, Trujillo, v. 14, n. 2, p. 201–212, 2023. Disponível em: https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/4842. Acesso em: 27 fev. 2026.

ESTÉVEZ, M. Protein carbonyls in meat systems: A review. Meat Science, Oxford, v. 89, n. 3, p. 259–279, 2011. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0309174011001616. Acesso em: 12 mar. 2026.

FALOWO, A. B.; FAYEMI, P. O.; MUCHENJE, V. Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Research International, Amsterdã, v. 64, p. 171–181, 2014. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0963996914004177. Acesso em: 12 mar. 2026.

FENG, Y. et al. Molecular mechanisms and applications of polyphenol-protein complexes with antioxidant properties: a review. Antioxidants, Basel, v. 12, n. 8, p. 1577, 2023. Disponível em: https://www.mdpi.com/2076-3921/12/8/1577. Acesso em: 12 mar. 2026.

FERREIRA, J. M. de S. et al. Parâmetros de qualidade avaliados em carne bovina: uma revisão. Brazilian Journal of Animal and Environmental Research, Curitiba, v. 6, n. 2, p. 1319–1332, 2023. Disponível em: https://ojs.brazilianjournals.com.br/ojs/index.php/BJAER/article/view/59810. Acesso em: 9 abr. 2026.

GENG, L.; LIU, K.; ZHANG, H. Lipid oxidation in foods and its implications on proteins. Frontiers in Nutrition, Lausanne, v. 10, p. 1192199, 2023. Disponível em: https://www.frontiersin.org/articles/10.3389/fnut.2023.1192199/full. Acesso em: 12 mar. 2026.

GHELICHI, S. et al. Oxidation and oxidative stability in emulsions. Comprehensive Reviews in Food Science and Food Safety, Chicago, v. 22, n. 3, p. 1864–1901, 2023. Disponível em: https://ift.onlinelibrary.wiley.com/doi/10.1111/1541-4337.13134. Acesso em: 12 mar. 2026.

INNOCENTE-ALVES, C. et al. Whey as a biotechnological resource: current trends and future directions. ACS Food Science & Technology, Washington, v. 5, n. 8, p. 2892–2906, 2025. Disponível em: https://pubs.acs.org/doi/10.1021/acsfoodscitech.5c00508. Acesso em: 2 mar. 2026.

JIANG, L. et al. A Review of whey protein-based bioactive delivery systems: design, fabrication, and application. Foods, Basel, v. 13, n. 15, p. 2453, 2024. Disponível em: https://www.mdpi.com/2304-8158/13/15/2453. Acesso em: 27 fev. 2026.

KHAJAVI, M. Z. et al. Functional meat products: the new consumer’s demand. Current Nutrition & Food Science, Sharjah, v. 16, n. 3, p. 260–267, 2020. Disponível em: http://dx.doi.org/10.2174/1573401315666190227161051. Acesso em: 27 fev. 2026.

KHETO, A. et al. A review on advancements in emerging processing of whey protein: Enhancing functional and nutritional properties for functional food applications. Food Safety and Health, Chicago, v. 3, n. 1, p. 23–45, 2025. Disponível em: https://iadns.onlinelibrary.wiley.com/doi/10.1002/fsh3.12067. Acesso em: 27 fev. 2026.

KRÓLCZYK, J. et al. Use of Whey and Whey Preparations in the Food Industry – a Review. Polish Journal of Food and Nutrition Sciences, [s. l.], v. 66, n. 3, p. 157–165, 2016. Disponível em: http://www.journalssystem.com/pjfns/Use-of-Whey-and-Whey-Preparations-in-the-Food-Industry-a-Review,98453,0,2.html. Acesso em: 9 out. 2024.

KUMAR, R. et al. Whey proteins: a potential ingredient for food industry- a review. Asian Journal of Dairy and Food Research, Karnal, n. of, 2018. Disponível em: http://arccjournals.com/journal/asian-journal-of-dairy-and-food-research/DR-1389. Acesso em: 27 fev. 2026.

LATIMER JUNIOR, G. W. Official methods of analysis of AOAC International. 20. ed. Rockyville: AOAC International, 2016.

LEE, S. et al. Recent strategies for improving the quality of meat products. Journal of Animal Science and Technology, London, v. 65, n. 5, p. 895–911, 2023. Disponível em: https://doi.org/10.5187/jast.2023.e94. Acesso em: 27 fev. 2026.

LUND, M. N. et al. Protein oxidation in muscle foods: A review. Molecular Nutrition & Food Research, Weinheim, v. 55, n. 1, p. 83–95, 2011. Disponível em: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201000453. Acesso em: 12 mar. 2026.

MAIA JÚNIOR, J. de A. et al. Efeito da redução do teor de gordura e NaCl nas características físico-químicas, cor e textura instrumental de linguiça defumada ovina. Brazilian Journal of Animal and Environmental Research, Curitiba, v. 3, n. 3, p. 1697–1712, 2020. Disponível em: https://www.brazilianjournals.com/index.php/BJAER/article/view/14836/12264. Acesso em: 9 abr. 2026.

MANESSIS, G. et al. Plant-derived natural antioxidants in meat and meat products. Antioxidants, Basel, v. 9, n. 12, p. 1215, 2020. Disponível em: https://www.mdpi.com/2076-3921/9/12/1215. Acesso em: 1 abr. 2026.

MIRZAKULOVA, A. et al. Whey: composition, processing, application, and prospects in functional and nutritional beverages - a review. Foods, Basel, v. 14, n. 18, p. 3245, 2025. Disponível em: https://www.mdpi.com/2304-8158/14/18/3245. Acesso em: 2 mar. 2026.

NOURMOHAMMADI, N.; AUSTIN, L.; CHEN, D. Protein-based fat replacers: a focus on fabrication methods and fat-mimic mechanisms. Foods, Basel, v. 12, n. 5, p. 957, 2023. Disponível em: https://www.mdpi.com/2304-8158/12/5/957. Acesso em: 2 mar. 2026.

PAGLARINI, C. de S. et al. Protein-based hydrogelled emulsions and their application as fat replacers in meat products: A review. Critical Reviews in Food Science and Nutrition, Philadelphia, v. 62, n. 3, p. 640–655, 2022. Disponível em: https://www.tandfonline.com/doi/full/10.1080/10408398.2020.1825322. Acesso em: 30 mar. 2026.

PAME, K. et al. Utilization of whey and whey based preparation in processing and development of value added low-fat meat products. International Journal of Current Microbiology and Applied Sciences, Tamil Nadu, v. 9, n. 12, p. 3308–3315, 2020. Disponível em: https://www.ijcmas.com/abstractview.php?ID=20838&vol=9-12-2020&SNo=394. Acesso em: 27 fev. 2026.

PAPUC, C. et al. Plant polyphenols as antioxidant and antibacterial agents for shelf‐life extension of meat and meat products: classification, structures, sources, and action mechanisms. Comprehensive Reviews in Food Science and Food Safety, Chicago, v. 16, n. 6, p. 1243–1268, 2017. Disponível em: https://ift.onlinelibrary.wiley.com/doi/10.1111/1541-4337.12298. Acesso em: 1 abr. 2026.

RUEDT, C.; GIBIS, M.; WEISS, J. Meat color and iridescence: origin, analysis, and approaches to modulation. Comprehensive Reviews in Food Science and Food Safety, [s. l.], v. 22, n. 4, p. 3366–3394, 2023. Disponível em: https://ift.onlinelibrary.wiley.com/doi/10.1111/1541-4337.13191. Acesso em: 3 mar. 2026.

RUIZ-CAPILLAS, C.; HERRERO, A. M. Novel strategies for the development of healthier meat and meat products and determination of their quality characteristics. Foods, Basel, v. 10, n. 11, p. 2578, 2021. Disponível em: https://www.mdpi.com/2304-8158/10/11/2578. Acesso em: 27 fev. 2026.

SAGHARI, V. et al. Evaluation of whey protein coating containing free and pickering emulsion forms of Trachyspermum copticum L . essential oil on quality of refrigerated beef. Food Science & Nutrition, Malden, v. 12, n. 11, p. 9187–9197, 2024. Disponível em: https://onlinelibrary.wiley.com/doi/10.1002/fsn3.4454. Acesso em: 3 mar. 2026.

SANTOS, M. M. F. et al. Lipid and protein oxidation of emulsified chicken patties prepared using abdominal fat and skin. Poultry Science, Cambridge, v. 99, n. 3, p. 1777–1787, 2020. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0032579119578245. Acesso em: 12 mar. 2026.

SHARMA, K.; CHAUHAN, E. S. Multifaceted whey protein: its applications in food industry. International Journal of Health Sciences and Research, [s. l.], v. 8, n. 10, p. 262–268, 2018. Disponível em: https://www.ijhsr.org/IJHSR_Vol.8_Issue.10_Oct2018/38.pdf. Acesso em: 27 fev. 2026.

SILVA, J. G. et al. Addition effects of bovine blood globin and sodium caseinate on the quality characteristics of raw and cooked ham pâté. Meat Science, Amsterdã, v. 63, n. 2, p. 177–184, 2003. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0309174002000682. Acesso em: 5 jan. 2026.

TORRES-MARTÍNEZ, B. D. M. et al. Natural ingredients to enhance the antioxidant capacity in different meat products: a review. Foods, Basel, v. 15, n. 5, p. 852, 2026. Disponível em: https://www.mdpi.com/2304-8158/15/5/852. Acesso em: 31 mar. 2026.

WU, M. et al. Rheological and microstructural properties of porcine myofibrillar protein–lipid emulsion composite gels. Journal of Food Science, Champaign, v. 74, n. 4, 2009. Disponível em: https://ift.onlinelibrary.wiley.com/doi/10.1111/j.1750-3841.2009.01140.x. Acesso em: 3 mar. 2026.

YESSIMBEKOV, Z. et al. Functional and sensory properties of pâtés formulated with wmulsions from chicken by-products. Foods, Basel, v. 14, n. 20, p. 3488, 2025. Disponível em: https://www.mdpi.com/2304-8158/14/20/3488. Acesso em: 3 mar. 2026.

YOUSSEF, M. K.; BARBUT, S. Effects of protein level and fat/oil on emulsion stability, texture, microstructure and color of meat batters. Meat Science, Amsterdã, v. 82, n. 2, p. 228–233, 2009. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0309174009000266. Acesso em: 3 mar. 2026.

YU, Q. P. et al. Studies on meat color, myoglobin content, enzyme activities, and genes associated with oxidative potential of pigs slaughtered at different growth stages. Asian-Australasian Journal of Animal Sciences, Seoul, v. 30, n. 12, p. 1739–1750, 2017. Disponível em: http://www.ajas.info/journal/view.php?doi=10.5713/ajas.17.0005. Acesso em: 6 mar. 2026.

ZHANG, K. et al. Covalent polyphenols-proteins interactions in food processing: formation mechanisms, quantification methods, bioactive effects, and applications. Frontiers in Nutrition, Lausanne, v. 11, p. 1371401, 2024. Disponível em: https://www.frontiersin.org/articles/10.3389/fnut.2024.1371401/full. Acesso em: 12 mar. 2026.

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2026-04-21

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Rodrigues, C. A. A., Dias, P. A., Silva, M. R., & Morais, H. A. (2026). Whey protein concentrate as a functional matrix in chicken pâté: impacts on physicochemical stability and lipid oxidation. Brazilian Journal of Animal and Environmental Research, 9(2), e86728. https://doi.org/10.34188/bjaerv9n2-043

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