Analysis of cortisol after sublingual ozone therapy

Abstract

Ozone therapy represents a versatile therapeutic approach with immunomodulatory and antibacterial properties, along with its ability to enhance tissue oxygenation. These attributes make it applicable across various clinical domains. Systemic administration of ozone has shown promising results in regulating biomarkers such as cortisol, positively influencing the inflammatory response and metabolic balance. Among the different administration routes, including rectal insufflation, topical application, intramuscular, and intravenous methods, the sublingual route emerges as a practical, safe, yet underexplored alternative with the potential to improve treatment accessibility. This study aimed to evaluate the immediate and systemic effects of sublingual ozone therapy using salivary cortisol levels as a biomarker. Materials and Methods: A cohort of 38 randomly selected volunteers received a single dose of 180 mL of medicinal ozone at a concentration of 25 mcg/L. Salivary cortisol levels were measured before and 10 minutes after administration. The results showed statistical significance (p=0.01), indicating a reduction in cortisol levels. Additionally, a stress scale applied prior to the intervention revealed that individuals with elevated cortisol levels also exhibited moderate stress. Systemic sublingual ozone therapy effectively reduced cortisol levels, demonstrating a rapid and clinically relevant therapeutic response. This method shows potential for managing stress-related conditions and metabolic imbalances, offering a practical and accessible extension of medicinal ozone applications.