Bioassay method for toxicity studies of toxic baits formulations to Spodoptera frugiperda (Lepidoptera: Noctuidae)
DOI:
https://doi.org/10.34117/bjdv12n3-031Keywords:
physiological status, semiochemicals, attract and kill, fall armyworm, IPMAbstract
Understanding the biological factors that act on the behavior of Spodoptera frugiperda (Lepidoptera: Noctuidae) adult is of importance for the development of management strategies based on the use of cairomones as food attractants. The objective of the study was to develop a bioassay methodology with the ready-to-use food attractant Noctovi® + insecticides on S. frugiperda adults. Based on the results, age (48 h = > 60% mortality), food deprivation (24 h = 70% mortality) and offering time (12 h > 50% mortality) have been shown to influence the susceptibility of S. frugiperda to toxic bait formulations with the food attractant Noctovi®. However, the availability of an artificial diet together with the toxic baits did not increase the mortality of S. frugiperda adults. In addition, the susceptibility of S. frugiperda varied depending on the lethal agent used in the formulation of the baits, with the lowest toxicity being observed for the insecticide Chlorpyrifos Nortox (mortality < 60%) in mixture with all the food attractants evaluated (Noctovi®, Acttra FAW and Acttra 43SB). In contrast, the formulations with Acttra FAW + Chlorantraniliprole and Acttra 43SB and Noctovi® in mixture with the insecticide Chlorfenapyr provided mortality of > 90%. The food deprivation for 24h, time spent offering the toxic bait for 12 h and the absence of an extra food source for 48-h-old S. frugiperda adults are suitable conditions for evaluating toxic bait formulations under laboratory conditions. In addition, the toxicity of bait formulations varies according to the insecticide used as the lethal agent.
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