Effect of NAA and BAP addition on the growth of Barangan banana subculture on MS media
DOI:
https://doi.org/10.34117/bjdv11n9-036Keywords:
BAP, Barangan banana, NAA, tissue cultureAbstract
Banana barangan is one of the potential banana species to be developed. In general, the development of pisang barangan is still conventional so that one step to overcome it is by using tissue culture techniques. This study aims to obtain the best concentration of NAA and BAP interaction for the growth of banana subculture on MS media. This research was conducted at the Biotechnology Laboratory of the Faculty of Agriculture, Tanjungpura University for 3 months, from April to July 2024. This study used a factorial complete randomized design (CRD) with two factors, namely NAA concentration (N) consisting of 3 levels (n1 = 0.5 ppm; n2 = 1 ppm; n3 = 1.5 ppm) and BAP concentration (B) consisting of 4 levels (b1 = 1 ppm; b2 = 2 ppm; b3 = 3 ppm; b4 = 4 ppm) so that 12 treatment combinations were obtained. Each treatment consisted of 3 replicates and each treatment unit consisted of 3 samples and each bottle was planted with 1 explant so that the total number of experimental units was 108 bottles. The results showed that the administration of NAA (0.5-1.5 ppm) and BAP (1-4 ppm) singly or the interaction concentration of both gave the same effect on the percentage of living explants, the time of first root appearance, the time of first shoot appearance, the number of shoots, the percentage of sprouted explants, and the percentage of rooted explants. The concentration of 1 ppm NAA is the best concentration to grow the roots of banana barangan explants on MS media.
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