Efecto bioestimulante de la microalga Scenedesmus dimorphus sobre plantines de tomate frente al déficit hídrico

Authors

  • Florencia Andrea Maseda
  • Diana Frezza
  • Claudia Mónica Ribaudo

DOI:

https://doi.org/10.34117/bjdv11n7-069

Keywords:

Solanum lycopersicum L., sustentabilidad, pigmentos fotosintéticos, fitohormonas, osmotina

Abstract

Los bioestimulantes son productos orgánicos que en bajas dosis estimulan el crecimiento y desarrollo de cultivos, activan distintas vías metabólicas e incrementan la tolerancia al estrés biótico y abiótico. El objetivo de este trabajo fue evaluar la respuesta en plantines de tomate (Solanum lycopersicum L), a la aplicación con Scenedesmus dimorphus y su potencial uso como bioestimulante. Los plantines fueron cultivados bajo invernadero con una restricción hídrica moderada. Se realizaron 4 tratamientos con 2 niveles de riego (100% y 50% de la capacidad del contenedor), con y sin aplicación de microalgas. Los plantines recibieron 3 mL del cultivo de microalgas junto con el riego (cuatro veces, durante 22 días que duró el experimento). La restricción hídrica fue a los 9 días desde la siembra. Se midieron las variables de crecimiento y contenido de pigmentos fotosintéticos, concentración de fitohormonas y la expresión de gen Sl-NP24 (osmotina). Se observó una mejora en las variables de crecimiento ante la aplicación de microalgas y activación de la respuesta de defensa en plantines con restricción hídrica, que fue mayor en los que no recibieron microalgas, evidenciada por mayor contenido de carotenoides, de ácido abscísico y sobreexpresión de Sl-NP24, mientras que, la aplicación de microalgas resultó en un efecto protector ante el déficit hídrico. El uso de S. dimorphus para plantas de tomate se presenta como una alternativa biológica y sustentable contra el estrés hídrico.

References

ALBACETE, A., GHANEM, M. E., MARTINEZ-ANDUJAR, C., ACOSTA, M., SANCHEZ-BRAVO, J., MARTINEZ, V. & PEREZ-ALFOCEA, F. (2008). Hormonal changes in relation to biomass partitioning and shoot growth impairment in salinized tomato (Solanum lycopersicum L.) plants. Journal of Experimental Botany, 59(15), 4119–4131. doi:10.1093/jxb/ern251

ALMEIDA-SILVA, F., & VENANCIO, T. M. (2022). Pathogenesis-related protein 1 (PR-1) genes in soybean. Gene, 809, 146013.

BASHIR MA, SILVESTRI C, AHMAD T, HAFIZ IA, ABBASI NA, MANZOOR A, CRISTOFORI V, RUGINI E. OSMOTIN: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants. Plants (Basel). 2020 Aug 4;9(8):992. doi: 10.3390/plants9080992. PMID: 32759884; PMCID: PMC7464907.

BRADFORD, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein. Analytical Biochemistry, 72(1-2), 248–254.

CHANDA M.J.., BENHIMA R., ELMERNISSI N., KASMI Y., KARIM, L., SBABOU L., YOUSSE., Z, y EL ARROUSSI, H.,. (2020). Screening of microalgae liquid extracts for their bio stimulant properties on plant growth, nutrient uptake and metabolite profile of Solanum lycopersicum L. Scientific Reports, 10(1). doi:10.1038/s41598-020-59840-4

DOBREV PI & KAMÍNEK M.(2002) Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction . J Chromatogr A. Mar 15;950(1-2):21-9. doi: 10.1016/s0021-9673(02)00024-9. PMID: 11990994.

FERREIRA, A.; MARQUES, P.; RIBEIRO, B.; ASSEMANY, P.; de MENDONÇA, H.V.; BARATA, A.; OLIVEIRA, A.C.; REIS, A.; PINHEIRO, H.M. y GOUVEIA, L. (2018) Combining biotechnology with circular bioeconomy: From poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogen. Environ. Res. 164, 32-38. doi:10.1016/j.envres.2018.02.007

FLORIDO BACALLAO, M., & BAO FUNDORA, L. (2014). Water stress tolerance in tomato. Cultivos Tropicales, 35(3), 70-88.

GERSZBERG, A., y HNATUSZKO-KONKA, K. (2017). Tomato tolerance to abiotic stress: a review of most often engineered target sequences. Plant growth regulation, 83, 175-198.

GHANEM ME, ALBACETE A, MARTÍNEZ-ANDÚJAR C, ACOSTA M, ROMERO-ARANDA R, DODD IC, LUTTS S & PÉREZ-ALFOCEA F. (2008). Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.). Journal of Experimental Botany 59, 3039-3050.

GLICK, B. R. (2005). Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS Microbiology Letters, 251(1), 1–7.

GOEL, D., SINGH, A. K., YADAV, V., BABBAR, S. B., & BANSAL, K. C. (2010). Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.). Protoplasma, 245(1-4), 133-141. doi:10.1007/s00709-010-0158-0

HAI, N. N., CHUONG, N. N., TU, N. H. C., KISIALA, A., HOANG, X. L. T., & THAO, N. P. (2020). Role and Regulation of Cytokinins in Plant Response to Drought Stress. Plants, 9(4), 422. doi:10.3390/plants9040422

HOYEROVÁ K, GAUDINOVÁ A, MALBECK J, DOBREV PI, KOCÁBEK T, SOLCOVÁ B, TRÁVNÍCKOVÁ A y KAMÍNEK M. Efficiency of different methods of extraction and purification of cytokinins. Phytochemistry. 2006 Jun;67(11):1151-9.

doi: 10.1016/j.phytochem.2006.03.010. Epub 2006 May 5. PMID: 16678229.

IKEGAMI, K., OKAMOTO, M., SEO, M., y KOSHIBA, T. (2009). Activation of abscisic acid biosynthesis in the leaves of Arabidopsis thaliana in response to water deficit. Journal of Plant Research, 122(2), 235-243. doi:10.1007/s10265-008-0201-9

INSKEEP, W. P., & BLOOM, P. R. (1985). Extinction coefficients of chlorophyll a and b. Plant Physiology, 77(2), 483–485.

KAPOORE, R. V., WOOD, E. E., y LLEWELLYN, C. A. (2021). Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices. Biotechnology Advances, 49, 107754. doi:10.1016/j.biotechadv.2021.107754

KUDO, T., KOBAYASHI, M., TERASHIMA, S., KATAYAMA, M., OZAKI, S., KANNO, M. y YANO, K. (2017). TOMATOMICS: A Web Database for Integrated Omics Information in Tomato. Plant and Cell Physiology, pcw207. doi:10.1093/pcp/pcw207

KUMAR, S. A., KUMARI, P. H., JAWAHAR, G., PRASHANTH, S., SURAVAJHALA, P., KATAM, R. y KISHOR, P. B. K. (2016). Beyond just being foot soldiers - osmotin like protein (OLP) and chitinase (Chi11) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato. Environmental and Experimental Botany, 132, 53-65. doi:10.1016/j.envexpbot.2016.08.007

KUSVURAN, A., & CAN, A. (2020). Effects of microalga (Chlorella vulgaris) on secondary metabolites and antioxidative defense system. Legume Research, 43(1), 56-60.

KUSVURAN, S., & DASGAN, H. Y. (2017). Drought induced physiological and biochemical responses in Solanum lycopersicum genotypes. Acta Scientiarum Polonorum Hortorum Cultus, 16(6), 19-27.

MARTINI F., BEGHINI G., ZANIN L., VARANINI Z. , ZAMBONI A. and BALLOTTARI M., The potential use of Chlamydomonas reinhardtii and Chlorella sorokiniana as biostimulants on maize plants, Algal Research, Volume 60, 2021, 102515, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2021.102515.

MONTEIRO, E.; GONÇALVES, B.; CORTEZ, I. y CASTRO, I. (2022) The Role of Biostimulants as Alleviators of Biotic and Abiotic Stresses in Grapevine: A Review. Plants, 11, 396. https://doi.org/10.3390/plants11030396

MUHAMMAD ASLAM, M.; WASEEM, M.; JAKADA, B.H.; OKAL, E.J.; LEI, Z.; SAQIB, H.S.A.; YUAN, W.; XU, W. y ZHANG, Q. (2022) Mechanisms of Abscisic Acid-Mediated Drought Stress Responses in Plants. Int. J. Mol. Sci. , 23, 1084. https://doi.org/ 10.3390/10.3390/ijms23031084

NAMBARA, E., & MARION-POLL, A. (2005). Abscisic acid biosynthesis and catabolism. Annual Review of Plant Biology, 56, 165–185.

NAVARRO-LÓPEZ, E., RUÍZ-NIETO, A., FERREIRA, A., ACIÉN, F. G., y GOUVEIA, L. (2020). Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater. Molecules, 25(3), 664. doi:10.3390/molecules25030664

NISHIYAMA, R., WATANABE, Y., FUJITA, Y., Le, D. T., KOJIMA, M., WERNER, T. y TRAN, L.-S. P. (2011). Analysis of Cytokinin Mutants and Regulation of Cytokinin Metabolic Genes Reveals Important Regulatory Roles of Cytokinins in Drought, Salt and Abscisic Acid Responses, and Abscisic Acid Biosynthesis. The Plant Cell, 23(6), 2169-2183. doi:10.1105/tpc.111.087395

ORTIZ-MORENO ML, SOLARTE-MURILLO LV, SANDOVAL-PARRA KX. (2020) Biofertilization with Chlorophyta and Cyanophyta: An alternative for organic food production. Acta biol. Colomb. 25(2):303-313. DOI: http://dx.doi.org/10.15446/abc.v25n2.77183

POSPISILOVA J, VAGNER M, MALBECK J, TRAVNIAKOVA A y BATKOVA P. (2005). Interactions between abscisic acid and cytokinins during water stress and subsequent rehydration. Biologia Plantarum 49, 533–540.

RACHIDI F., BENHIMA R., KASMI Y., SBABOU, L., EL ARROUSSI, H. (2019) Effect of microal- gae polysaccharides on biochemical and metabolomics pathways related to plant defense in Solanum lycopersicum. Appl Bio- chem Biotechnol 188:225–240. https ://doi.org/10.1007/s1201 0-018-2916-y

RIBAUDO CM, RIVA DS, GORI JI, ZABALLA JI, MOLINA C (2016) Identification of Endophytic Bacteria and their Characterization as Biocontrol Agents against Tomato Southern Blight Disease. Appli Micro Open Access 2: 1000123. doi:10.4172/2471-9315.1000123

RIVA, D. S., & RIBAUDO, C. M. (2020). Inoculation with Pseudomonas pseudoalcaligenes enhances tolerance against pathogenic fungus in tomato. American Scientific Research Journal for Engineering, Technology, and Sciences, 69(1), 89-104.

ROUPHAEL, Y., & COLLA, G. (2020). Editorial: Biostimulants in agriculture. Frontiers in Plant Science, 11, 1-7.

RStudio Team (2020). RStudio: Integrated Development for R. RStudio, PBC, Boston, MA URL http://www.rstudio.com/

SALCEDO, M. F., COLMAN, S.L.; MANSILLA, A.Y.; MARTÍNEZ, M.A; FIOL, D. F.; ALVAREZ, V. A.; CASALONGUÉ, C. A. (2020). Amelioration of tomato plants cultivated in organic-matter impoverished soil by supplementation with Undaria pinnatifida. Algal Research, 46(), 101785–. doi:10.1016/j.algal.2019.101785

SANG-MOO Lee, BONGSOO Lee, CHANG-KI Shim, YONG-KEUN Chang y CHOONG-MIN Ryu (2020): Plant anti-aging: Delayed flower and leaf senescence in Erinus alpinus treated with cell-free Chlorella cultivation medium, Plant Signaling y Behavior, DOI: 10.1080/15592324.2020.1763005

SCHNEIDER, C., RASBAND, W. y ELICEIRI, K. (2012). NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9, 671–675 https://doi.org/10.1038/nmeth.2089

STEIN, J. R. (1973). Handbook of phycological methods. Cambridge University Press, 56-60.

SUCHITHRA M.R., DURAI MURUGAN MUNISWAMI, M., SUBA SRI, R., USHA, A. AHAMED RASHEEQ, B. ANTROSE PREETHI y DINESHKUMAR R.. (2022) Effectiveness of green microalgae as biostimulants and biofertilizer through foliar spray and soil drench method for tomato cultivation,South African Journal of Botany, Volume 146,Pages 740-750, ISSN 0254-6299, https://doi.org/10.1016/j.sajb.2021.12.022.

TAMBURINO, R., VITALE, M., RUGGIERO, A., SASSI, M., SANNINO, L., ARENA, S., COSTA, A., BATELLI, G., ZAMBRANO, N., SCALONI, A., GRILLO, S., y SCOTTI, N. (2017). Chloroplast proteome response to drought stress and recovery in tomato ( Solanum lycopersicum L .). 1–14. https://doi.org/10.1186/s12870-017-0971-0

TEKLIĆ, T., PARAĐIKOVIĆ, N., ŠPOLJAREVIĆ, M., ZELJKOVIĆ, S., LONČARIĆ, Z., y LISJAK, M. (2020). Linking abiotic stress, plant metabolites, biostimulants and functional food. Annals of Applied Biology. doi:10.1111/aab.12651

TEZCAN, A., ASLAN, G. E., y KAMAN, H. (2019). Evaluation of drought stress on the chlorophyll content of the plants: A review of the solanaceae family. Fresenius Environ. Bull, 28, 4636-4641.

THANG, T., BUI, V., y TRAN, T H. (2020). Effect of Drought Stress and Thermal Pre-treatment on the In vitro Shoot Development of Solanum lycopersicum L. Chemical Engineering Transactions. 78. 2020. 10.3303/CET2078039.

TRAN T.T., BUI V.T. y TRAN H.T., (2020), Effect of Drought Stress and Thermal Pre-treatment on the In vitro Shoot Development of Solanum lycopersicum L., Chemical Engineering Transactions, 78, 229-234 DOI:10.3303/CET2078039

TURIEL, S.; GARRIDO-CARDENAS, J.A.; GÓMEZ-SERRANO, C.; ACIÉN, F.G.; CARRETERO-PAULET, L. y BLANCO, S. A (2021) Polyphasic Characterisation of Tetradesmus almeriensis sp. nov. (Chlorophyta: Scenedesmaceae). https://doi.org/ 10.3390/pr9112006

WANG S., MENG Y., LIU J., CAO X., XUE S. Accurate quantification of astaxanthin from Haematococcus pluvialis using DMSO extraction and lipase-catalyzed hydrolysis pretreatment, Algal Research, Volume 35, 2018, Pages 427-431, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2018.08.029.

Weber, R., Wiebke-Strohm, B.; Bredemeier, C., Margis-Pinheiro, M., de Brito, G, Rechenmacher, C,. Bertagnolli, P., de Sá, Maria; Campos, M., de Amorim, R., Beneventi, M.,; Margis, R., Grossi-de-Sa, M., Bodanese-Zanettini, M. (2014).

Expression of an osmotin-like protein fromSolanum nigrumconfers drought tolerance in transgenic soybean. BMC Plant Biology, 14(1), 343–. doi:10.1186/s12870-014-0343-y

YEMM, E. W., & WILLIS, A. J. (1954). The estimation of carbohydrates in plant extracts by anthrone. The Biochemical Journal, 57(3), 508–514.

ZHANG, M., HAN, Y., LI, D., XU, S., & HUANG, Y. (2024). Smart horticulture as an emerging interdisciplinary field combining novel solutions: Past development, current challenges, and future perspectives. Horticultural Plant Journal, 10(6), 1257-1273.

Published

2025-07-29

How to Cite

Maseda, F. A., Frezza, D., & Ribaudo, C. M. (2025). Efecto bioestimulante de la microalga Scenedesmus dimorphus sobre plantines de tomate frente al déficit hídrico. Brazilian Journal of Development, 11(7), e81238. https://doi.org/10.34117/bjdv11n7-069

Issue

Section

Original Papers