Biological behavior of led photobiomodulation in different cell types
DOI:
https://doi.org/10.34117/bjdv11n1-011Keywords:
photobiomodulation, LED, cells, in vitroAbstract
The use of photobiomodulation is well documented in the literature, with evidence of its various therapeutic applications. However, there is a gap in the biological effects of LED on different cell types. Therefore, the aim of this study was to evaluate the effects triggered by different wavelengths of LED on different cell types. For this, 2x104 cells per well of adipocytes, melanocytes, fibroblasts, and endothelial cells were cultured in 75 cm2 T-flasks (bottle brand) containing DEMEM medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Hyclone), 1% penicillin/streptomycin antibiotic, L-glutamine, and bicarbonate (Sigma-Aldrich). T-flasks were maintained at 37°C with 5% CO2 in an oven (Thermo Scientific). For the application of phototherapy, wavelengths of 410 nm, 450 nm, 525 nm, 590 nm, 630 nm and 850 nm were used in a single application, with evaluations performed 24 hours after irradiation. The results demonstrated that high energies using red and infrared wavelengths reduced adipocyte proliferation. When low energies at the same wavelengths were used in fibroblasts, greater cell proliferation was observed. Regarding melanocytes, only the 590 nm wavelength was able to induce a response related to cell proliferation. Thus, it was possible to conclude that the biological response is dependent on both the wavelength and the amount of energy applied, and differences in light absorption were also detected depending on the cell type used.
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