Pomegranate plant tissue culture technique - a review
DOI:
https://doi.org/10.34117/bjdv10n11-022Keywords:
micropropagation, pomegranate, in vitro, virus free plantsAbstract
Background: The pomegranate, or Punica granatum L., is a member of the "Punicaceae" family, which also includes Punica protopunica and Punica granatum. Originating in Afghanistan and Iran, it has spread over Asia, Africa, and Europe's Mediterranean region. The traditional method of pomegranate propagation is laborious and time-consuming. It does not guarantee healthy, disease-free plants. Its shortcomings include poor success rates, extremely sluggish replication, and a one-year establishing period for new plants. Tissue culture is a technique that can be used to grow plants in all seasons without restrictions. Several pomegranate-growing nations have tried in vitro propagation to generate virus-free, similar-type plants, and it has been widely used for propagating many fruit trees. Research Purpose: The goal of this essay was to examine how to do plant tissue culture method and get familiar with the techniques. with an emphasis on the pomegranate micropropagation. The only aspect of plant tissue culture that may be able to get around these issues is in vitro technology. Therefore, there is plenty of room for employing micropropagation to multiply desired genotypes on a wide scale. Research Method: This review article was conducted based on scientific websites in Persian and English languages, including SID, Magiran, Google Scholar, Web of Science, and Library Studies, Results: Pomegranate plants multiply quickly through micropropagation, producing plants (clones) that are genetically similar. In a short amount of time, a single explant can yield thousands of plants. These plants match exactly. The study provides a standardized protocol for in vitro regeneration of nodal explants, which could be useful in large-scale production of uniform and disease-free plantlets. The process of plant tissue culture reduces the need for importing expensive plant seeds.
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