Modelo animal minimamente invasivo para implante em côndilos femorais de coelhos
DOI:
https://doi.org/10.34117/bjdv10n11-002Keywords:
modelo animal em coelho, côndilos femorais, implantes ósseosAbstract
Objetivo: O presente estudo teve como principal intento criar Modelo Animal que permitisse avaliação de reação óssea em implantes de maneira minimamente invasiva. Metodologia: Em região condiliana lateral e medial do fêmur em joelho de coelho fixou-se, com guia específico, implante intra ósseo de 7 x 2,5 mm. Resultados e discussão: Coelhos são animais conhecidos e os muito utilizados para avaliação de reação óssea. Sua resposta inflamatória (reação periosteal) densidade mineral óssea dos ossos longos são comparáveis aos dos humanos. Fazendo um Modelo Animal com menor agressão e padronização nos implantes premiaríamos menor dano ao animal e maior replicabilidade na amostra pesquisada. Conclusão: Conseguiu-se formatar Modelo Animal que se mostrou de execução simples e reproduzível.
References
Rebollo RA.; O legado Hipocrático e sua fortuna no período greco-romano: de Cós a Galeno. Scientiae Studia [online]. 2006; v. 4, n.1; 45-81
Fagundes DJ. e Taha MO.: Modelo animal de doença: critérios de escolha e espécies de animais de uso corrente. Acta Cirúrgica Brasileira [online]. 2004; v.19, n.1.
Ye L., Shu-Kui C., Long L., Ling Q., Xin-Luan W., Yu-Xiao L.; Bone defect animal models for testing efficacy of bone substitute biomaterials. review article Journal of Orthopaedic Translation (2015) 3, 95-104
Zwingenberger S, Niederlohmann E, Vater C, Rammelt S, Matthys R, Bernhardt R, et al. Establishment of a femoral critical-size bone defect model in immunodeficient mice. J Surg Res 2013; 181; 7-14.
Bateman JP, Safadi FF, Susin C, Wikesjo UM. Exploratory study on the effect of osteoactivin on bone formation in the rat critical-size calvarial defect model. J Periodontal Res 2012; 47: 243-247.
Zhang X, Cai Q, Liu H, Heng BC, Peng H, Song Y, et al. Osteoconductive effectiveness of bone graft derived from antler cancellous bone: an experimental study in the rabbit mandible defect model. Int J Oral Maxillofac Surg 2012;41: 1330-1337.
Schneider G, Blechschmidt K, Linde D, Litschko P, Korbs T, Beleites E. Bone regeneration with glass ceramic implants and calcium phosphate cements in a rabbit cranial defect model. J Mater Sci Mater Med 2010; 21: 2853-2859.
Yano K, Namikawa T, Uemura T, Hoshino M, Wakitani S, Takaoka K, et al. Regenerative repair of bone defects with osteoinductive hydroxyapatite fabricated to match the defect and implanted with combined use of computer-aided design, computer-aided manufacturing, and computer- assisted surgery systems: a feasibility study in a canine model. J Orthop Sci 2012; 17: 484-489.
Takigami H, Kumagai K, Latson L, Togawa D, Bauer T, Powell K, et al. Bone formation following OP-1 implanta- tion is improved by addition of autogenous bone marrow cells in a canine femur defect model. J Orthop Res 2007; 25:1333-1342.
Yang HL, Zhu XS, Chen L, Chen CM, Mangham DC, Coulton LA, et al. Bone healing response to a synthetic calcium sulfate/beta-tricalcium phosphate graft material in a sheep vertebral body defect model. J Biomed Mater Res B Appl Biomater 2012; 100: 1911-1921.
Reichert JC, Epari DR, Wullschleger ME, Saifzadeh S, Steck R, Lienau J, et al. Establishment of a preclinical ovine model for tibial segmental bone defect repair by applying bone tissue engineering strategies. Tissue Eng Part B Rev 2010;16: 93-104.
Lippens E, Vertenten G, Girones J, Declercq H, Saunders J, Luyten J, et al. Evaluation of bone regeneration with an injectable, in situ polymerizable Pluronic F127 hydrogel derivative combined with autologous mesenchymal stem cells in a goat tibia defect model. Tissue Eng Part A 2010;16: 617-627.
Yu D, Li Q, Mu X, Chang T, Xiong Z. Bone regeneration of critical calvarial defect in goat model by PLGA/TCP/rhBMP-2 scaffolds prepared by low-temperature rapid-prototyping technology. Int J Oral MaxillofacSurg 2008; 37: 929-934.
Wehrhan F, Amann K, Molenberg A, Lutz R, Neukam FW, Schlegel KA. PEG matrix enables cell-mediated local BMP-2 gene delivery and increased bone formation in a porcine critical size defect model of craniofacial bone regeneration. Clin Oral Implants Res 2012; 23: 805-813.
Springer IN, Acil Y, Kuchenbecker S, Bolte H, Warnke PH, Abboud M, et al. Bone graft versus BMP-7 in a critical size defectd cranioplasty in a growing infant model. Bone 2005; 37:563-569.
Rahman CV, Ben-David D, Dhillon A, Kuhn G, Gould TW, Muller R, et al. Controlled release of BMP-2 from a sintered polymer scaffold enhances bone repair in a mouse calvarial defect model. J Tissue Eng Regen Med 2014; 8: 59-66.
Das A, Tanner S, Barker DA, Green D, Botchwey EA. Delivery of S1P receptor-targeted drugs via biodegradable polymer scaffolds enhances bone regeneration in a critical size cra- nial defect. J Biomed Mater Res A 2014; 102:1210-18.
Li R, Nauth A, Li C, Qamirani E, Atesok K, Schemitsch EH. Expression of VEGF gene isoforms in a rat segmental bone defect model treated with EPCs. J Orthop Trauma 2012; 26: 689-92.
Cheng C, Alt V, Dimitrakopoulou-Strauss A, Pan L, Thormann U, Schnettler R, et al. Evaluation of new bone formation in normal and osteoporotic rats with a 3-mm femur defect: functional assessment with dynamic PET-CT (dPET- CT) using 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG) and (18)F-fluoride. Mol Imaging Biol 2013; 15: 336-44.
Mohan BG, Shenoy SJ, Babu SS, Varma HK, John A. Strontium calcium phosphate for the repair of leporine (Oryctolagus cuniculus) ulna segmental defect. J Biomed Mater Res A 2013; 101: 261-71.
Kim A, Kim DH, Song HR, Kang WH, Kim HJ, Lim HC, et al. Repair of rabbit ulna segmental bone defect using freshly isolated adipose-derived stromal vascular fraction. Cyto- therapy 2012; 14: 296-305.
Schlegel KA, Lang FJ, Donath K, Kulow JT, Wiltfang J. The monocortical critical size bone defect as an alternative experimental model in testing bone substitute materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102: 7-13.
Liebschner MA. Biomechanical considerations of animal models used in tissue engineering of bone. Biomaterials 2004; 25: 1697-1714.
Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG. An- imal models for implant biomaterial research in bone: a re- view. Eur Cell Mater 2007; 13: 1-10.
Upman PJ. ISO 10993-6: test for local effects after implantation. Bonezone 2006; 5: 50-2.
FAGUNDES, Djalma José; TAHA, Murched Omar. Modelo animal de doença: critérios de escolha e espécies de animais de uso corrente. Acta Cirúrgica Brasileira, v. 19, p. 59-65, 2004.
Neyt JG, Buckwalter JA, Carroll NC. Use of animal models in musculoskeletal research. Iowa Orthop J. 1998;18:118-23. PMID: 9807717; PMCID: PMC2378160.
Pearce A, Richards RG, Milz S, Schneider E, Pearce SG. Animal models for implant biomaterial research in bone: a review. Eur Cell Mater 2007;13:1–10
Fagundes D, Taha M. Choice ́s criteria and current animals spec- imens. Acta Cir Bras 2004;19:59–65.
Burkholder, T, Linton, G, Hoyt, RJ. The rabbit as an experimental model. In: Suckow MA, Stevens KA and Wilson RP (editors). The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents; 2012.
Wang X, Mabrey JD, Agrawal CM. An interspecies comparison of bone fracture properties. Biomed Mater Eng. 1998;8(1):1-9.
Mäkitaipale J, Sievänen H, Laitinen-Vapaavuori O. Tibial bone density, cross-sectional geometry and strength in Finnish PET rabbits: a peripheral quantitative computed tomography study. Vet Rec 2018;183:382.
Castañeda S, Largo R, Calvo E, et al. Bone mineral measurements of subchondral and trabecular bone in healthy and osteoporotic rabbits. Skeletal Radiol. 2006;35(1):34-41. doi:10.1007/s00256-005-0022-z
Graur D, Duret L, Gouy M. Phylogenetic position of the order Lago- morpha (rabbits, hares and allies). Nature 1996;379:333–335.
Gatin L, Saleh-Mghir A, Massin P, Crémieux AC. Critical analysis of experimental models of periprosthetic joint infection. Orthop Traumatol Surg Res 2015;101:851–855.
Wancket LM. Animal models for evaluation of bone implants and devices: comparative bone structure and common model uses. Vet Pathol 2015;52:842–850.
Li Y, Chen SK, Li L, Qin L, Wang XL, Lai YX. Bone defect animal models for testing efficacy of bone substitute biomaterials. J Orthop Translat. 2015 Jun 16;3(3):95-104. doi: 10.1016/j.jot.2015.05.002. PMID: 30035046; PMCID: PMC5982383.
Masoud I, Shapiro F, Kent R, Moses A. A longitudinal study of the growth of the New Zealand white rabbit: cumulative and biweekly incremental growth rates for body length, body weight, femoral length, and tibial length. J Orthop Res 1986;4:221–231.
Chai H, Guo L, Wang X, Fu Y, Guan J et al. Antibacterial effect of 317L stainless steel contained copper in prevention of implant- related infection in vitro and in vivo. J Mater Sci Mater Med 2011;22:2525–2535.
Bottagisio M.; Coman C.; Lovati A.B. Animal models of orthopaedic infections. A review of rabbit models used to induce long bone bacterial infections. Journal of Medical Microbiology 2019;68:506–537 DOI 10.1099/jmm.0.000952

