Performance of zebu donor cows in vitro production of embryos / Desempenho de vacas doadoras zebu na produção in vitro de embriões
DOI:
https://doi.org/10.34117/bjdv6n2-182Keywords:
Bovine, Biotechniques, IVF, ovum pick up.Abstract
In vitro production (IVP) of embryos is a biotechnology capable of increase bovine production. Thus, the performance of Brahman, Gir, and Nellore donor cows in IVP was evaluated. Data from 2012 to 2017 were collected from donors subjected to ovum pick up (OPU) for IVP at the In Vitro Acre Company, Rio Branco, Acre, Brazil. The numbers of aspirated oocytes, viable oocytes, viable embryos, and pregnancy per OPU, and the cleavage, viable embryo, and pregnancy rates were analyzed considering the breed, location, time, number of OPU/day, and number of OPU/donor. The numbers were compared by the Student t test, and the rates by the chi-square test. Brahman donors presented higher rates of cleavage, and viable embryos, followed by Nellore, and Gir (p<0.05), and lower number of pregnancies (p<0.05). Donors raised in the state of Acre, Brazil, had higher numbers of aspirated oocytes, viable oocytes, and viable embryos (p<0.05), whereas those raised in Peru had higher cleavage and pregnancy rates (p<0.05). Pregnancy rates were higher in the rainy season (p<0.05). Cleavage rates were higher when more than 20 OPU/day were performed (p<0.05), and intermediate when performing 11-20 OPU/day. The numbers of aspirated and viable oocytes of donors subjected to one OPU were higher than those of donors subjected to four to seven OPU (p<0.05). Cleavage rates of donors subjected to two OPU were higher than those of donors subjected to more than three OPU (p<0.05). The breed, location, number of OPU/day, and number of OPU performed in the same donor affected, with different intensities, the performance variables of Brahman, Gir, and Nellore donors in IVP.
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