Qualitative and quantitative evaluation of the comparison between the conventional and adapted spot on the law method

Authors

  • Gabriele Silva Dias
  • Guilherme Brito Vianna
  • Gabriella Costa Ribeiro
  • Claúdio Henrique Gonçalves Barbosa
  • Adriano Sakai Okamoto
  • Raphael Lucio Andreatti Filho

DOI:

https://doi.org/10.34117/bjdv10n2-024

Keywords:

spot on the law, probiotic, bacterium

Abstract

The spot on the law technique is of great importance for the study of probiotic bacteria. Therefore, the main objective of this research was to develop an adaptation of this technique with the prerogative of making its application more practical and executable on a large scale in addition, validating the adaptation of this methodology based on the quantitative and qualitative analysis of the data obtained. For statistical analysis, we used the Mann-Whitney Rank Sum Test, analogous to the T Test for non-parametric data. From the results obtained, it was possible to verify that the adaptation is equivalent to the classic technique, as there was no statistical difference between the two, and that the application of the adapted methodology facilitates the execution of the spot because it presents a lower degree of difficulty.

References

ANEE, I. J., ALAM, S., BEGUN, A. R., SHAHJAHAN, M. R., KHANDAKER, M. A. The role of probiotcs on animal health and nutrition. The Journal of Basic and Applied Zoology, 2021.

BAUER, A. W., KIRBY, W. M., SHERRIS, J. C., TRUCK, M. Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology, 1966.

BELICOVÁ, A., MIKULÁSOVÁ, M., DUSINSKÝ, R. Probiotic potential and safety properties of Lactobacillus plantarum from Slovak Bryndza cheese. Biomedical Research International, 2013.

BRASIL, Instrução Normativa número 171 de 13 de dezembro de 2018. Informa sobre a intensão de proibição de uso de antimicrobianos com a finalidade de aditivos melhoradores de desempenho de alimentos e abre prazo manifestação. Brasília: 13 de dezembro de 2004.

DIAS, G. S., VIANNA, G. G., RIBEIRO, G. C., OKAMOTO, A. S., ANDREATTI FILHA, R. L. Adaptation of the spot on the law techinique in the analyses of probiotic in inhibiting pathogenic bactéria growth. Facta, Campinas – SP, 2023.

FAO [Food and Agriculture Organization of the United Nations]. Meat & Meat Products. 2014. Disponível em: http://www.fao.org/ag/againfo/themes/e n/meat/home.html.

HWANHLEM, N., SALIPETH, L., CHAROENSOOK, R., KANJAN, P., MANEERAT, S. Lactic acid bacteria from gamecock and goat originating from Phitsanulok, Thailand: Isolation, identification, technological properties and probiotic potential. Journal Microbiology Biotechnology, 2022.

JIMENEZ-TRIGO, E., TOQUET, M., BARBA, M., GÓMEZ-MARTÍN, Á., QUEREDA, J. J., BATALLER, E. Search of antimicrobial lactic acid bacteria from Salmonella-negative dogs. BMC Veterinary Research, 2022.

KALHORO, M. S., ANAL, A. K., KALHORO, D. H., HUSSAIN, T., MURTAZA, G., MANGI, M. H. Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk. Oxidative Medicine Cellular Longevity, 2023.

LEWIS, C.B.; KAISER, A.; MONTVILLE, T.J. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bactéria isolated from meat. Journal Microbiology Biotechnology, v.57, p.1683-1688. 1991.

LILLY, D. M., STILLWELL, R. H, Probiotics: growth promoting factors produced by microorganisms. Science. 1965.

MABEKU. L. B., NGUE S., BONSOU NGUEMO, I., LEUNDJI, H., EUNDJI, H. Potential of selected lactic acid bacteria from Theobroma cacao fermented fruit juice and cell-free supernatants from cultures as inhibitors of Helicobacter pylori and as good probiotic. BMC Research Notes, 2020.

MENDONÇA, L. O. A., CALDAS, E. R., ROSA, S. A., LELES, A. C. R., PARESOTO, L. M., CUNHA, R. R., FILHO, F. M. V., ZANOL, T. S., SILVA, G. F., DORNELLES, E. A., FERREIRA, M. V., MARCELINO, E. C., CAIXETA, F. C., BALDUINO, T. L. P. Prevenção de infecções por microrganismos multirresistentes em serviços de saúde: uma revisão de literatura. Brazilian Journal of Development, 2023.

MONTEIRO, C. R. A.V., CARMO, M. S., MELO, B. O., ALVES, M. S., SANTOS, C. I., Monteiro, S. G., BONFIM, M. R. Q., FERNANDES, E. S., MONTEIRO-NETO, V., In Vitro Antimicrobial Activity and Probiotic Potential of Bifidobacterium and Lactobacillus against Species of Clostridium. Nutrients, 2019.

OKAMOTO, A. S., ANDREATTI FILHO, R. L., MILBRADT, E. L., MORAES, A. C. I., VELHANO, I. H. B., GUIMARÃES-OKAMOTO, P. T. C. Bacterial communication between Lactobacillus spp. isolated from poultry in the inhibition of Salmonella Heidelberg - proof of concept. Poultry Science, 2018.

OMS. Organização Mundial da Saúde. Dia Mundial da Saúde, 7 de abril de 2011. Disponível em: http://www.who.int/world-health-day/2011/es

OMS. Organização Mundial da Saúde. Novo relatório apela a medidas urgentes para prevenir crises causadas pela resistência antimicrobiana. Genebra, 2019.

OMS. Organização Mundial da Saúde. Dia Mundial da Saúde. Global Antimicrobial Resistance and Use Surveillance System (GLASS) REPORT, 2022.

RIBEIRO, G. C., MOGOLLÓN-GARCÍA, H. D., MORAES, A. C. I., DIAS, G. S., VIANNA, G. B., MILBRADT, E. L., ANDREATTI FILHO, R. L., OKAMOTO, A. S. Research Note: The effects of a Lactobacillus helveticus ATCC 15009-derived postbiotic mitigating Salmonella Gallinarum colonization in commercial layer chicks. Poultry Science, 2023.

SANDHIYA, T. Potential drug target from breast milk Lactobacillus against vaginal Pathogens. Brazilian Journal of Development, Curitiba, 2024

UNIÃO EUROPEIA, REGULAMENTO (UE) 2019/6 DO PARLAMENTO EUROPEU E DO CONSELHO de 11 de dezembro de 2018 relativo aos medicamentos veterinários e que revoga a Diretiva 2001/82/CE. Jornal da União Europeia, 2019.

Published

2024-02-15

How to Cite

Dias, G. S., Vianna, G. B., Ribeiro, G. C., Barbosa, C. H. G., Okamoto, A. S., & Andreatti Filho, R. L. (2024). Qualitative and quantitative evaluation of the comparison between the conventional and adapted spot on the law method. Brazilian Journal of Development, 10(2), e67225. https://doi.org/10.34117/bjdv10n2-024

Issue

Section

Original Papers