Cytotoxicity of polybia paulista wasp crude venom on the tumor cell lines HeLa, HRT-18, jurkat, and K562
DOI:
https://doi.org/10.34117/bjdv10n1-043Keywords:
Polybia sp., cell viability, antitumor, carcinoma, LeukemiaAbstract
The social wasp Polybia paulista is an animal that produces venom for defense, which has been the subject of numerous studies in recent decades. Despite the great scientific interest, its pharmacological and biotechnological potential is still unknown. In this study, the antitumor capacity of the crude venom of P. paulista was investigated through cytotoxicity assays on the cell lines HeLa, HRT-18, Jurkat, and K562. Based on the cytotoxicity assays performed after 24 hours of treatment, the results showed that the crude venom was able to significantly reduce cell proliferation and induce cell death in the K562, HRT-18, and Jurkat cell lines. Morphological and cell cycle analyses in the Jurkat cell line revealed evidence of chromatin fragmentation and the presence of early and late apoptotic cells. It can be concluded that the crude venom of the social wasp P. paulista is cytotoxic to the tumor cell lines K562 (chronic myeloid leukemia), Jurkat (acute lymphocytic leukemia), and HRT-18 (intestinal carcinoma). The obtained results report the cytotoxic effects of the venom of the wasp P. paulista and demonstrate the importance of prospecting this venom, which is still poorly investigated.
References
ALHAKAMY, N.A.; AHMED, O.A.A.; SHADAB, M.; FAHMY, U.A. Mastoparan, a peptide venom conjugated fluvastatin nanocomplex for suppression of lung cancer cell growth. Polymers, v.13, p.4225-4239. 2021.
AZEVEDO, R. A.; FIGUEIREDO, C. R.; FERREIRA, A. K.; MATSUO, A. L.; MASSAOKA, H.; GIROLA, N.; AUAD, A. V. V.; FARIAS, C. F.; PASQUALOTO, K. F. M.; RODRIGUES, C. P.; BARBUTO, J. A.; LEVY, D.; BYDLOWSKI, S. P.; TRAVASSOS, L. R.; LEBRUN, I. Mastoparan induces apoptosis in B16F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo. Peptides, v68, p.113-119, 2015.
BRADFORD, M. A. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-bonding. Anal. Biochem. v.72, p.248-254, 1976.
COSTA-LOTUFO, L. V. et al. A Contribuição dos Produtos Naturais como Fonte de Novos Fármacos Anticâncer: Estudos no Laboratório Nacional de Oncologia Experimental da Universidade Federal do Ceará .Rev. Virtual Quim. |Vol 2| |No. 1| |47-58|.
DONGOL, T.; DHANANJAYA, B.L.; SHRESTHA, R.K.; ARYAL, G. Pharmacological and immunological properties of wasp venom. In Pharmacology and Therapeutics; Joghi, S., Gowder, T., Eds Intech: Rijeka, Croatia, p.47-81, 2014.
DOS SANTOS CABRERA, M.P.; COSTA, S.T.B.; DE SOUZA, B.M.; PALMA, M.S.; RUGGIERO, J.R.; RUGGIERO-NETO, J. Selectivity in the mechanism of action of antimicrobial mastoparan peptide Polybia-MP1. Jornal Europeu de Biofísica, v.37, p.879-891, 2008.
JANG, MI-HYEON; SHIN, M; LIM, S; HAN, S; PARK, H; SHIN, I. LEE, J; KIM, K; KIM, E; KIM, C. Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299. Journal of pharmacological sciences, Coréia, v. 91, n. 2, p. 95-104, 2003.
KHOZEIMEH, F.; GOLESTANNEJAD, Z.; DOOSTMOHAMMADI, M., GAVANJ, S. Investigating the effect of bee venom on human colon cancer cells (HT-29) and hepatic cells (HepG2) in comparison L929 cells. Journal of Chemical and Pharmaceutical Research, Irã, v. 7, p. 1-5, 2015.
LEE, Y. J; KANG, S, J; KIM, B. M; KIM, Y. J; WOO, H. D; CHUNG, H. W. Cytotoxicity oh Honeybee (Apis mellifera) venom in normal human lymphocytes and HL-60 cells, Chemico-Biological Interactions, Corea, v. 169, p. 189-19, 2007.
MACHADO, E. K. Estudo do efeito dos derivados de imidas cíclicas sobre os mecanismos de resistência tumoral e apoptose em células de linhagens tumorais. 177p. Tese - Universidade Federal de Santa Catarina. Florianópilis, 2013.
MACHADO, V. L. L., Análise populacional de colônias de Polybia (Myrapetra) paulista (Ihering, 1896) (Hymenoptera, vespidae). Revista brasileira de Zoologia, v.2, n.4, p.187-201, São Paulo 1984.
MAEDA, L. Avaliação da Citotoxicidade da fase Vapor do óleo Essencial de pogostemon cablin benth. em Células de Adenocarcinoma de Pulmão - calu-3. P, 47, Monografia (Farmácia) – Universidade Estadual de Ponta Grossa. 2016
MORENO, M et GIRALD, E. Three Valuable Peptides from Bee and Wasp Venoms for Therapeutic and Biotechnological Use: Melittin, Apamin and Mastoparan. Toxins. 2015, 7(4), 1126-1150
ONUCHIC, A. C., & CHAMMAS, R. (2010). Câncer e o microambiente tumoral. Revista De Medicina, 89(1), 21-31
RIBBLE, D. et al. A simple technique for quantifying apoptosis in 96-well plates. BMC Biotechnology, v. 5, n. 1, p 12-19, 2005
ROCHA, T.; DE SOUZA, B.M.; PALMA, M.S.; DE CRUZ-HOFLING, M.A. Myotoxic effects of mastoparan from Polybia paulista (Hymenoptera, Epiponini) wasp venom in mice skeletal muscle. Toxicon., v.50, p.589-599, 2007.
WANG, K.; ZHANG,B.; ZHANG, W.; YAN,J.; LI,J.; WANG, R. Antitumor effects, cell selectivity and structure-activity relationship os a novel antimicrobial peptide polybia-MPI. Peptides, v.29, p.963-968, 2008.
YONG-WAN, K.; CHATURVEDI, P.K.; CHUN, S.N.; LEE, Y.G.; AHN, W.S. Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line. Oncology reports, v.33, p.1675-1682, 2015.
