Avaliação de métodos titulométricos para determinação do grau de desacetilação em quitosana/ Evaluation of titulometric methods for determination of deacetylation´s degree in chitosan
DOI:
https://doi.org/10.34117/bjdv6n1-289Keywords:
Titulação, Quitosana, Grau de DesacetilaçãoAbstract
A quitina é o segundo biopolímero mais abundante depois da celulose, é encontrada nos exoesqueletos de crustáceos, insetos e moluscos, sendo a sua estrutura composta de amino-polissacarídeo linear. A quitosana é um polissacarídeo catiônico, sendo produzido a partir da desacetilação da quitina, caracterizada principalmente pelo grau de desacetilação e pelo peso molecular, sendo que esses parâmetros afetam fortemente suas propriedades físico-químicas e biológicas, tais como solubilidade, hidrofilicidade e cristalinidade. O grau de desacetilação pode ser obtido por titulação potenciométrica e condutivimétrica, sendo empregado basicamente para diferenciar quitina e quitosana, uma vez que a presença de grupos amino na estrutura da quitosana é o indicador que confere a este polímero muitas propriedades específicas. Em virtude disso, esta pesquisa teve como objetivo comparar métodos de titulação já descritos na literatura, para determinar o grau de desacetilação ( ) de uma amostra de quitosana sintetizada no Laboratório de Sólidos Inorgânicos em comparação com uma amostra obtida comercialmente.
References
ABDULKARIM, A. et al. Extraction and Characterization of Chitin and Chitosan from Mussel Shell. Natural Products Chemistry & Research, v. 3, n. 2, p. 108–114, 2013. https://www.researchgate.net/publication/280520739
AL-MANHEL, A. J.; AL-HILPHY, A. R. S.; NIAMAH, A. K. Extraction of chitosan, characterisation and its use for water purification. Journal of the Saudi Society of Agricultural Sciences, v. 17, n. 2, p. 186–190, 2018. https://doi.org/10.1016/j.jssas.2016.04.001
BAXTER, A. et al. Improved method for I.R. determination of the degree of acetylation of chitosan. Int. J. Biol. Macromol., v. 14, p. 166–169, 1992. https://doi.org/10.1016/S0141-8130(05)80007-8
COCOLETZI, H. H. et al. Obtención y caracterización de quitosano a partir de exoesqueletos de camarón. Superficies y Vacío, v. 22, n. 3, p. 57–60, 2009. http://smcsyv.fis.cinvestav.mx/supyvac/22_3/SV2235709.pdf
CZECHOWSKA-BISKUP, R. et al. Determination of Degree of Deacetylation of Chitosan - Comparaison of Methods. Progress on chemistry and application of chitin and its derivatives, v. 17, p. 5–20, 2012. https://journals.indexcopernicus.com/search/article?articleId=90457
DEMITRI, C. et al. Nanostructured active chitosan-based films for food packaging applications: Effect of graphene stacks on mechanical properties. Measurement: Journal of the International Measurement Confederation, v. 90, p. 418–423, 2016.
http://dx.doi.org/10.1016/j.measurement.2016.05.012
EL KNIDRI, H. et al. Extraction, chemical modification and characterization of chitin and chitosan. International Journal of Biological Macromolecules, v. 120, p. 1181–1189, 2018. https://doi.org/10.1016/j.ijbiomac.2018.08.139
ESCOBAR-SIERRA, D. M. et al. Manufacturing and evaluation of Chitosan, PVA and Aloe Vera hydrogels for skin applications. DYNA, v. 84, n. 203, p. 134–142, 2017.
https://doi.org/10.15446/dyna.v84n203.62742
FRICK, J. M. et al. Influence of Glutaraldehyde Crosslinking and Alkaline Post-treatment on the Properties of Chitosan-Based Films. Journal of Polymers and the Environment, v. 26, n. 7, p. 2748–2757, 2018. http://dx.doi.org/10.1007/s10924-017-1166-3
GEROMEL-COSTA, C. G. A. et al. Adsorption of metals by crosslinked chitosan beads in sugarcane contaminated streams. Biomass and Bioenergy, v. 119, n. September, p. 128–134, 2018. https://doi.org/10.1016/j.biombioe.2018.09.019
HOMEZ-JARA, A. et al. Characterization of chitosan edible films obtained with various polymer concentrations and drying temperatures. International Journal of Biological Macromolecules, v. 113, p. 1233–1240, 1 jul. 2018. https://doi.org/10.1016/j.ijbiomac.2018.03.057
HUSSEIN, M. H. M. et al. Preparation of Some Chitosan Heavy Metal Complexes and Study of Its Properties 1. Natural Polymers, v. 54, n. 2, p. 113–124, 2012. https://doi.org/10.1134/S0965545X12020046
KUMARI, S. et al. Extraction and characterization of chitin and chitosan from fishery waste by chemical method. Environmental Technology and Innovation, v. 3, p. 77–85, 2015. https://doi.org/10.1016/j.eti.2015.01.002
KUMIRSKA, J. et al. Application of spectroscopic methods for structural analysis of chitin and chitosan. Marine Drugs, v. 8, n. 5, p. 1567–1636, 2010. https://doi.org/10.3390/md8051567
KYZAS, G. Z.; KOSTOGLOU, M. Swelling-adsorption interactions during mercury and nickel ions removal by chitosan derivatives. Separation and Purification Technology, v. 149, p. 92–102, 2015. http://dx.doi.org/10.1016/j.seppur.2015.05.024
MENDHAM, J. et al. Análise Química Quantitativa. 6. ed. Rio de Janeiro, RJ: LTC, 2002.
MOHAMMADZADEH PAKDEL, P.; PEIGHAMBARDOUST, S. J. Review on recent progress in chitosan-based hydrogels for wastewater treatment application. Carbohydrate Polymers, v. 201, p. 264–279, 2018. https://doi.org/10.1016/j.carbpol.2018.08.070
MORAN, H. B. T. et al. Immunomodulatory properties of chitosan polymers. Biomaterials, v. 184, p. 1–9, 2018. https://doi.org/10.1016/j.biomaterials.2018.08.054
MUJTABA, M. et al. Current advancements in chitosan-based fi lm production for food technology?; A review. International Journal of Biological Macromolecules, v. 121, p. 889–904, 2019. https://doi.org/10.1016/j.ijbiomac.2018.10.109
MUXIKA, A. et al. Chitosan as a bioactive polymer: Processing , properties and applications. International Journal of Biological Macromolecules, v. 105, p. 1358–1368, 2017. https://doi.org/10.1016/j.ijbiomac.2017.07.087
RAYMOND, L.; MORIN, F. G.; MARCHESSAULT, R. H. Degree of deacetylation titration and solid-state of chitosan using conductometric NMR. Carbohydrate Research, v. 246, p. 331–336, 1993. https://doi.org/10.1016/0008-6215(93)84044-7
RINAUDO, M. Chitin and chitosan: Properties and applications. Progress in Polymer Science (Oxford), v. 31, n. 7, p. 603–632, 2006. https://doi.org/10.1016/j.progpolymsci.2006.06.001
RINAUDO, M.; MILAS, M.; LE DUNG, P. Characterization of chitosan. Influence of ionic strength and degree of acatylation on chain expansion . International Journal of Biological Macromolecules, v. 15, p. 281–285, 1993. https://doi.org/10.1016/0141-8130(93)90027-J
ROBERTS, G. A. F. Chitin Chemistry. London, UK: Macmillan Press Ltd, 1982.
ROY, J. C. et al. Solubility of Chitin: Solvents, Solution Behaviors and Their Related Mechanisms. In: Solubility of Polysaccharides. [s.l: s.n.]. p. 20. https://doi.org/10.5772/intechopen.71385
SIGMA-ALDRICH. Quitosana da casca de camarão com peso molecular médio. Disponível em: https://www.sigmaaldrich.com/catalog/product/aldrich/448877. Acesso em: 13 jan. 2020.
SKOOG, D. A.; HOLLER, F. J.; NIMAN, T. A. Princípios de Análise Instrumental. 5. ed. São Paulo, SP: Bookman, 2002.
WANG, M. et al. The green adsorption of chitosan tripolyphosphate nanoparticles on cotton fiber surfaces. Carbohydrate Polymers, v. 101, n. 1, p. 812–818, 2014. http://dx.doi.org/10.1016/j.carbpol.2013.10.023
WANG, X. et al. Preparation of pure chitosan film using ternary solvents and its super absorbency. Carbohydrate Polymers, v. 153, 2016. http://dx.doi.org/10.1016/j.carbpol.2016.07.081
YOUNES, I.; RINAUDO, M. Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine Drugs, v. 13, n. 3, p. 1133–1174, 2015.
http://dx.doi.org/10.3390/md13031133
ZENG, D.; LUO, X.; TU, R. Application of Bioactive Coatings Based on Chitosan for Soybean Seed Protection. International Journal ofCarbohydrate Chemistry. v. 2012, 2012. http://dx.doi.org/10.1155/2012/104565
ZENG, L. et al. Adsorption of Cd ( II ), Cu ( II ) and Ni ( II ) ions by cross-linking chitosan/rectorite nano-hybrid composite microspheres. Carbohydrate Polymers, v. 130, p. 333–343, 2015. http://dx.doi.org/10.1016/j.carbpol.2015.05.015
ZVEZDOVA, D. Synthesis and characterization of chitosan from marine sources in. Synthesis and characterization of chitosan from marine sources in Black Sea, v. 3, n. 1, p. 65–69, 2010. https://conf.uni-ruse.bg/bg/docs/cp10/9.1/9.1-11.pdf
