Multiplication <em>in vitro</em> of strawberry plant under different types of LEDs
DOI:
https://doi.org/10.34117/bjdv9n1-099Keywords:
explant, micropropagation, light qualityAbstract
Use of light-emitting diodes (LEDs) in strawberry in vitro multiplication The refinement of plant tissue culture techniques is of great importance for optimizing in vitro multiplication rates and improving the quality of micropropagated changes, either light-emitting diodes (LEDs) or the latest models of light-emitting light sources light..The objective of this work was to evaluate the effect of light type on in vitro multiplication of Tudla strawberry cultivar. Strawberry sprouts were grown for 30 days in MS culture medium plus 100 mg L-1 of myo-inositol, 7 g L-1 agar, 1.0 mg L-1 BAP and 30 g L-1 sucrose. The pH of the culture medium was adjusted to 5.8 prior to the addition of agar. The controls used were composed of three light types [(green LEDs - EDET 3LA1 530 nm, red LEDs - EDER 3LA3 630 nm and fluorescent lamps (control treatment)]. Note that the average number of sprouts formed by red LEDs (11 The buds /explant) was higher than ears under fluorescent lamps (7.8 buds /explant), the lowest average was observed under fluorescent lamps, but there was no difference between explants grown in green LEDs and red LEDs in vitro shoot of strawberry cultivar Tudla.
References
BULA, R. J. et al. Light-emitting diodes as a radiation source for plants. HortScience, Wisconsin, v. 26, p. 203-205, feb. 1991 Available at: https://www.researchgate.net/publication/11808175_Light-emitting_Diodes_as_a_Radiation_Source_for_Plants/link/0912f5097ed185b7b1000000/download Accessed: 04 Aug. 2022.
Leticia Kurchaidt Pinheiro. Productivity and quality of strawberry cultivars in organic and conventional systems in the region of Guarapuava-PR. Dissertation (Master in Plant Production), Central-West State University, Guarapuava, 2008.
CHANG, H. S. et al. Micropropagation of Gallia lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. In Vitro Cellular & Developmental Biology-Plant, Columbia, v. 39 p. 129-134, Mar. 2003. DOI:10.1079/IVP2002362 Available at: https://link.springer.com/article/10.1079%2FIVP2002362 Accessed: 04 Aug. 2022.
KIM, S. J. et al. Effects of LEDs on net photosynthetic rate, growth and leaf stomara of Chysanthemum plantlets in vitro. Scientia Horticulturae, Amsterdam, v. 101, p. 143-151. 2004. DOI: https://doi.org/10.1016/j.scienta.2003.10.003 Available at: https://www.sciencedirect.com/science/article/abs/pii/S0304423803001730 Accessed: 04 Aug. 2022.
NHUT, D. T.; NAM, N. B. Light-emitting diodes (LEDs): An artificial lighting source for biological studies. IN: 3RD INTERNATIONAL CONFERENCE ON THE DEVELOPMENT OF BIOMEDICAL ENGINEERING IN VIETNAM, IFMBE Proceedings... Berlin, 2010. Available at: https://link.springer.com/chapter/10.1007/978-3-642-12020-6_33. Accessed: 04 Aug. 2022.
NHUT, D. T. et al. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue and Organ Culture, The Hague, v. 73, p. 43-52, April. 2003. Available at: https://link.springer.com/article/10.1023/A:1022638508007 Accessed: 04 Aug. 2022.
MUNEER, S. et al. Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.). International Journal of Molecular Sciences, Berlin, v. 15(3), p. 4657-4670, Mar. 2014. DOI: https://www.mdpi.com/1422-0067/15/3/4657 Available at: https://www.mdpi.com/1422- 0067/15/3/4657/htm Retrieved: 04 Aug. 2022.
MURASHIGE T.; SKOOG F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, Copenhagen, v. 15, p. 473-497, april. 1962 Available at: http://priede.bf.lu.lv/grozs/AuguFiziologijas/Augu_audu_kulturas_MAG/literatura/03_Murashige%20Scoog1962.pdf Accessed: 04 Aug. 2022.
OLIVEIRA, R. P.; SCIVITTARO, W. B. Strawberry fruit production due to different periods of vernalization of the seedlings. Brazilian Horticulture, Brasilia, v. 27, no. 1, pp. 91-95, Jan.-Mar. 2009. DOI: https://doi.org/10.1590/S0102-05362009000100018 Available at: https://www.scielo.br/pdf/hb/v27n1/18.pdf Accessed: 04 Aug. 2022.
POUDEL, P. et al. Effect of red and blue light-emitting diodes on growth and morphogenesis of grapes. Plant Cell, Tissue and Organ Culture, The Hague, v. 92, para. 2, pp. 47-153, feb. 2008 DOI:10.1007/s11240-007-9317-1 Available at: https://link.springer.com/article/10.1007%2Fs11240-007-9317-1 Accessed: 04 Aug. 2022.
RAMÍREZ-MOSQUEDA, M. A.; IGLESIAS-ANDREU, L. G.; BAUTISTA-AGUILAR, J. R. The effect of light quality on growth and development of in vitro plantlet of Stevia rebaudiana Bertoni. Sugar Tech, Jankipuram, v. 1, p. 1-6, June 2016. Available at: https://link.springer.com/article/10.1007%2Fs12355-016-0459-5 Accessed: 04 Aug. 2022.
ROCHA, P. S.G. et al. Light-emitting diodes and BAP concentrations in the in vitro multiplication of strawberry. Rural Science, Santa Maria, v. 40, p. 1922-1928, Sep. 2010. https://doi.org/10.1590/S0103-84782010000900011 Available at: https://www.scielo.br/pdf/cr/v40n9/a699cr2985.pdf Accessed: 04 Aug. 2022.
SHIN, K. S. et al. The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plant. Acta Physiologia Plantarum, Berlin, v. 30, p. 339-343, feb. 2008 DOI 10.1007/s11738-007-0128-0 Available at: https://link.springer.com/content/pdf/10.1007/s11738-007-0128-0.pdf Accessed: 04 Aug. 2022.
TOMAR, U. K. et al. An overview on economic factors influencing micropropagation. My Forest, Jodhpur, v. 43, p. 523-534, Jan. 2007. Available at: https://www.researchgate.net/publication/236149384_An_Overview_of_the_Economic_Factors_Influencing_Micropropagation Accessed: 04 Aug. 2022.
WU, J.H. et al. Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell, Tissue and Organ Culture, The Hague, v. 99, pp. 17-25, Aug. 2009. Available at: https://www.academia.edu/229914/An_Overview_of_the_Economic_Factors_Influencing_Micropropagation Accessed: 04 Aug. 2022
