Is there a relationship between the expression of β-catenin and Ki-67 in canine melanocytic neoplasms?
Existe relação entre a expressão de β-catenina e Ki-67 em neoplasias melanocíticas caninas?
DOI:
https://doi.org/10.34117/bjdv8n9-058Keywords:
melanocytic neoplasms, melanoma, β-catenin, Ki-67, immunohistochemistryAbstract
An immunohistochemical evaluation of 26 cutaneous and oral benign and malignant canine melanocytic neoplasms was performed to identify a possible relationship between the expression of the β-catenin molecule with cell proliferation using Ki-67 expression. This molecule is a component of the Wnt/β-catenin signalling pathway, which causes a cascade of intracellular events that activate transduction genes and nuclear transcription. The microscopic evaluation was performed considering the β-catenin labelling site (cytoplasmic, nuclear or mixed). The mitotic index was evaluated by the expression of Ki-67 in 10 high power fields (HPF) (400x). Statistically significant difference was neither detected between the β-catenin labelling sites and the various neoplasms, nor a correlation between the β-catenin molecule and the cell proliferation marker Ki-67 in the oral or cutaneous, benign or malignant neoplasms. Our study brings interesting findings and points to future research on this topic, especially with established variables in the clinical, pathological and immunohistochemical fields.
References
BERGIN, I.; SMEDLEY, R.; ESPLIN, D.; SPANGLER, W.; KIUPEL, M. Prognostic evaluation of Ki67 threshold value in canine oral melanoma. Veterinary Pathology, 48, 41-53, 2011.
CHON, E.; THOMPSON, V.; SCHMID, S.; STEIN, T. Activation of the Canonical Wnt/β-Catenin Signalling Pathway is Rare in Canine Malignant Melanoma Tissue and Cell Lines. Journal of Comparative Pathology, 148, 178-187, 2013.
GOLDSCHMIDT, M.H.; GOLDSCHMIDT, K.H. Epithelial and Melanocytic Tumors of the Skin. In: Tumors in Domestic Animals. 5th Edit., DJ Meuten, Ed., Wiley-Blackwell, Ames, Iowa State, pp. 88-141, 2017.
HAN, J-I.; KIM, D-Y.; NA, K-J. Dysregulation of the wnt/β-catenin signaling pathway in canine cutaneous melanotic tumor. Veterinary Pathology, 47, 285-291, 2010.
HAN, J-I.; KIM, Y.; KIM, D-Y.; NA, K-J. Alteration in E-cadherin/β-catenin expression in canine melanotic tumors. Veterinary Pathology, 50, 274-280, 2013.
LACROUX, C.; RAYMOND-LETRON, I.; BOURGES-ABELLA, N.; LUCAS, M.; DEVIERS, A.; SERRA, F. et al. Study of canine cutaneous melanocytic tumours: Evaluation of histological and immunohistochemical prognostic criteria in 65 cases. Revista de Medicina Veterinária, 163, 393, 2012.
LAPRIE, C.; ABADIE, J.; AMARDEILH, M.F.; NET, J.L.; LAGADIC, M.; DELVERDIER, M. MIB‐1 immunoreactivity correlates with biologic behaviour in canine cutaneous melanoma. Veterinary Dermatology, 12, 139-147, 2001.
LIU, J.; FUKUNAGA-KALABIS, M.; LI, L.; HERLYN, M. Developmental pathways activated in melanocytes and melanoma. Archives Biochemistry Biophysics, 563, 13-21, 2014.
LUCERO, O.M.; DAWSON, D.W.; MOON, R.T.; CHIEN, A.J. A re-evaluation of the “oncogenic” nature of Wnt/β-catenin signaling in melanoma and other cancers. Current Oncology Reports, 12, 314-318, 2010.
MACDONALD, B.T.; TAMAI, K.; HE, X. Wnt/β-catenin signaling: components, mechanisms, and diseases. Developmental Cell, 17, 9-26, 2009.
MILLANTA, F.; FRATINI, F.; CORAZZA, M.; CASTAGNARO, M.; ZAPPULLI, V.; POLI, A. Proliferation activity in oral and cutaneous canine melanocytic tumours: correlation with histological parameters, location, and clinical behaviour. Research in Veterinary Science, 73, 45-51, 2002.
NISHIYA, A.T.; MASSOCO, C.O.; FELIZZOLA, C.R.; PERLMANN, E.; BATSCHINSKI, K.; TEDARDI, M.V. et al. Comparative aspects of canine melanoma. Veterinary Science, 3, 7, 2016.
RAMOS-VARA, J.; BEISSENHERZ, M.; MILLER, M.; JOHNSON, G.; PACE, L.; FARD, A. et al. Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases. Veterinary Pathology, 37, 597-608, 2000.
ROLIM, V.M.; CASAGRANDE, R.A.; WATANABE, T.T.; WOUTERS, A.T.; WOUTERS, F.; SONNE, L. et al. Amelanotic melanoma in dogs: a retrospective study of 35 cases (2004-2010) and immunohistochemical characterization. Pesquisa Veterinária Brasileira, 32, 340-346, 2012.
SINNBERG, T.; MENZEL, M.; EWERTH, D.; SAUER, B.; SCHWARZ, M.; SCHALLER, M. et al. β-Catenin signaling increases during melanoma progression and promotes tumor cell survival and chemoresistance. PloS One, 6, e23429, 2011.
SMEDLEY, R.; SPANGLER, W.; ESPLIN, D.; KITCHELL, B.; BERGMAN, P.; HO, H-Y. et al. Prognostic markers for canine melanocytic neoplasms: a comparative review of the literature and goals for future investigation. Veterinary Pathology, 48, 54-72, 2011.
SMITH, S.; GOLDSCHMIDT, M.; MCMANUS, P. A comparative review of melanocytic neoplasms. Veterinary Pathology, 39, 651-678, 2002.
SPANGLER, W.; KASS, P. The histologic and epidemiologic bases for prognostic considerations in canine melanocytic neoplasia. Veterinary Pathology, 43, 136-149, 2006.
SULAIMON, S.S.; KITCHELL, B.E. The basic biology of malignant melanoma: molecular mechanisms of disease progression and comparative aspects. Jounal of Veterinary Internal Medicine, 7, 760-772, 2003.
VALENTA, T.; HAUSMANN, G.; BASLER, K. The many faces and functions of beta-catenin. EMBO Journal, 31, 2714-2736, 2012.
