Stevia Rebaudiana fraction presents broad-spectrum antibacterial action and antibiofilm action for Staphylococcus aureus / Fração Stevia Rebaudiana apresenta ação antibacteriana de amplo espectro e ação antibiótica para Staphylococcus aureus
DOI:
https://doi.org/10.34117/bjdv8n7-057Keywords:
phytotherapy, microbial resistance, biofilm, Stevia fraction, antimicrobial action.Abstract
Fractions of S. rebaudiana leaves, extracted in Ethyl Acetate (EAF), rich in antioxidant compounds and Isobutanolic fraction (IF), with a high content of glycosides, were evaluated for antibacterial and antibiofilm potential against reference strains of Gram-positive and Gram-negative bacteria of clinical interest. EAF had bactericidal action against Gram-positive strains evaluated and against most Gram-negative strains, with the exception of Salmonella enteritidis. Gram-positive species showed greater sensitivity to EAF. For IF, up to the maximum concentration, there was no bactericidal effect for the strains. The pretreatment of planktonic cells of S. aureus with subinhibitory concentrations of EAF determined a reduction in the potential of structuring of the biofilm by up to 71%. The fraction showed effectiveness in biofilm treatments formed by S. aureus, with a 99% reduction in cell viability compared to the control. In addition to the antibacterial action on planktonic cells, EAF has promising effects against S. aureus biofilms.
References
Ahmad J, Khan I, Blundell R, Azzopardi J, Mahomoodally MF (2020). Stevia rebaudiana Bertoni.: an updated review of its health benefits, industrial applications and safety. Trends in Food Science & Technology. 100: 177-189
Akthar MS, Degaga B, Azam T (2014). Antimicrobial activity of essential oils extracted from medicinal plants against the pathogenic microorganisms: a review. Journal Issues. 2350: 1588
Brambilla E, Cagetti MG, Ionescu A, Campus G, Lingström P (2014). An in vitro and in vivo comparison of the effect of Stevia rebaudiana extracts on different caries-related variables: A randomized controlled trial pilot study. Caries Ressearch. 48: 19–23
César de la FN, Reffuveille F, Fernández L, Hancock REW (2013). Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies. Current Opinion in Microbiology. 16: 580-589
Choi YM, Noh DO, Cho HJS, Kim KM, Kim JM (2006). Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT - Food Science and Technology. 39: 756e761
De Slavutzky SMB (2010). Stevia and sucrose effect on plaque formation. Journal für Verbraucherschutz und Lebensmittelsicherheit. 5: 213-216
Giacaman RA, Campos P, Muñoz-Sandoval C, Castro RJ (2013). Cariogenic potential of commercial sweeteners in an experimental biofilm caries model on enamel. Archieves of Oral Biology. 58: 1116–1122
Gupta PD, Birdi TJ (2017). Development of botanicals to combat antibiotic resistance. Journal of Ayurveda and Integrative Medicine. 8: 266-275
Hastuty A (2019). Antibiofilm and antimicrobial activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. Journal of Microbial Systematics and Biotechnology. 1: 19-29
Jayaraman S, Manoharan MS, Illanchezian S (2008). In-vitro Antimicrobial and Antitumor Activities of Stevia Rebaudiana (Asteraceae) Leaf Extracts Tropical Journal of Pharmaceutical Research. 7: 1143-1149
Jones SM, Morgan M, Humphrey TJ, Lappin-Scott H (2001). Effect of vancomycin and rifampicin on meticillin resistant Staphylococcus aureus biofilms. Lancet. 357: 40-1
Joseph, D.; George, J (2019). Remedial potentials of sweet leaf: a review on Stevia rebaudiana. Methods. 1
Latarissa IR, Barliana MI, Lestari K. A (2020). Comprehensive Review of Stevia rebaudiana Bertoni effects on Human Health and Its Mechanism. Journal of Advanced Pharmacy Education & Research. 10
Lebeaux D, Ghigo JM, Beloina C (2014). Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics. Microbiology and Molecular Biology Reviews. 510 –543
Milani PG, Formigoni M, Dacome AS, Benossi L, Costa CEM, Costa SC (2017a). New seminal variety of Stevia rebaudiana: Obtaining fractions with high antioxidant potential of leaves. Anais da Academia Brasileira de Ciências. 89: 1841-1850
Milani PG, Formigoni M, Lima YC, Piovan S, Peixoto G, Camparsi DM, da Silva Rodrigues W, da Silva J, da Silva Avincola A, Pilau EJ, da Costa CEM, da Costa SC (2017b). Fortification of the whey protein isolate antioxidant and antidiabetic activity with fraction rich in phenolic compounds obtained from Stevia rebaudiana (Bert.). Bertoni leaves. Journal of Food Science and Technology 54: 2020–2029 doi: 10.1007/s13197-017-2638-0.
Milani PG, Piovan S, Lima YC, Zorzenon MRT, da Rosa CVD, Peixoto GML. Mathias PCF, Natali MRM, da Costa SC, Mareze-Costa CE (2020). Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats. Journal of Food Science Technology. 58: 805–810
Mohire NC, Yadav AV(2010). Chitosan-based polyherbal toothpaste: As novel oral hygiene product. Indian Journal of Dental Research. 21: 380–384
Piovan S, Pavanello A, Peixoto GML, Matiusso CCI, de Moraes AMP, Martins IP, Malta A, Palma-Ringo K, Franco CCS, Milani PG, Dacome AS, Costa SC, Mathias PCF, Mareze-Costa CE (2018). Stevia nonsweetener fraction displays an insulinotropic effect involving neurotransmission in pancreatic islets. Int J Endocrinol 2018:1–7. https://doi.org/10.1155/2018/3189879.
Archer NSMJM, Costerton JW, Leid JG, Powers ME, Shirtliff ME (2011). Staphylococcus aureus biofilms Properties, regulation, and roles in human disease. Virulence 2: 445-459
Noor J, Razia K, Siraj A (2014). In vitro evaluation of antibacterial potential of Stevia rebaudiana Bertoni against various bacterial pathogens including resistant isolates with bla genes. Medicinal Plants-International Journal of Phytomedicines and Related Industries 6: 114-119
O'Gara JP (2017). Into the storm: Chasing the opportunistic pathogen Staphylococcus aureus from skin colonisation to life‐threatening infections. Environmental microbiology. 19: 3823-3833
Paraje MG (2011). Antimicrobial resistance in biofilms. Science against Microbial Pathogens: Communicating Current Research and Technological Advances. 9: 736–744
Rodis N, Tsapadikou VK, Potsios C, Xaplanteri P(2020). Resistance Mechanisms in Bacterial Biofilm Formations: A Review. Journal of Emergency and Internal Medicine. 4: 30
Singh S, Garg V, Yadav D, Beg MN, Sharma N (2012). In-vitro antioxidative and antibacterial activities of various parts of Stevia rebaudiana (Bertoni). International Journal of Pharmacy and Pharmaceutical Sciences. 4: 468-473
Singh B, Singh JP, Kaur A, Singh N (2018). Phenolic compounds as beneficial phytochemicals in pomegranate (Punica granatum L.) peel: A review. Food Chemistry 261: 75-86
Suresh MK, Biswas R, Biswas L (2018). An update on recent developments in the prevention and treatment of Staphylococcus aureus biofilms. International Journal of Medical Microbiology. 309: 1-12
World Health Organization (WHO) Fact Sheets: Antibiotic resistance (2020). https://www.who.int/en/news-room/fact-sheets/detail/antibiotic-resistance.
Yamaguchi, N.; Satoh-Yamaguchi, K.; Ono, M (2009). In vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components (Humulus lupulus) addressing acne vulgaris. Phytomedicine, 16(4):369-376.
Zorzenon MRT, Hodas F, Milani, PG, Formigoni M, Dacome AS, Monteiro ARG, Mareze-Costa CE, Costa SC (2019). Microencapsulation by Spray-Drying of Stevia Fraction with Antidiabetics Effects. Chemical Engineering Transactions. 75: 307-312 .
