Análise da metilação de genes em resposta a Diploidia em Aspergillus (=Emericella) Nidulans (Eidam) Vuill / Analysis of gene methylation in response of Diploidy in Aspergillus (=Emericella) Nidulans (Eidam) Vuill
DOI:
https://doi.org/10.34117/bjdv8n6-360Keywords:
Epigenética, inativação de genes, desenvolvimento das colônias.Abstract
Epigenética estuda alterações de expressão gênica sem alteração em nucleotídeos da região promotora. A metilação tem sido muito estudada em eucariotos superiores e mais recentemente em fungos. Aspergillus nidulans é um fungo filamentoso, homotálico geralmente haplóide. Por meio de cruzamentos com marcadores nutricionais é possível obter linhagens diplóides estáveis. Colônias diplóides apresentam desenvolvimento vegetativo e conidiogênese normalmente como ocorre com as haplóides. O presente trabalho teve como objetivo estudar a metilação de genes em resposta ao excesso de material genético em linhagens diplóides. Linhagens com marcadores conhecidos foram utilizadas para a obtenção dos diplóides biA1methG1//MSE, CLB3//MSE, ppy//MSE, 4//8 e 4//14. Colônias destes diplóides foram cultivadas para observação da ocorrência espontânea de setores com diferentes morfologias, coloração de conídios, formato dos conidióforos, presença de cleistotécios, indicativos de metilação em resposta a diploidia. Nenhuma das 100 colônias de cada diplóide produziu setores nem de coloração nem de esporulação nem de cleistotécios. Os diplóides foram cultivados com carência de oxigênio para a produção de cleistotécios. Ascósporos de um cleistotécio de cada linhagem foram inoculados para a obtenção de linhagens metiladas por evento pré-meiótico. Destas colônias foram selecionados setores para coloração. As colônias purificadas destes setores mostraram estabilidade de cor e grande parte delas tinham também uma alta produção de cleistotécios em resposta a metilação. Os resultados mostraram que linhagens diplóides ao serem ativadas sofrem metilação em genes para coloração de esporos e para a produção de ciclo sexual.
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