Produção de biocompostos microalgais em diferentes condições de cultivo / Production of microalgae biocompounds in different cultivation conditions

Authors

  • Marcele L. Nörnberg
  • Pricila N. Pinheiro
  • Tatiele C. do Nascimento
  • Andrêssa S. Fernandes
  • Eduardo Jacob Lopes
  • Leila Q. Zepka

DOI:

https://doi.org/10.34117/bjdv8n2-119

Keywords:

Compostos bioativos, Cultivo microalgal, Produção de carotenoides.

Abstract

Existe um consenso mundial na indústria de alimentos que visa a substituição de ingredientes sintéticos por ingredientes naturais para promoção da saúde. Ao mesmo tempo, está em constante crescimento a relevância industrial das microalgas como fontes de um extensivo espectro de bioprodutos e como promissoras matérias-primas para produção de aditivos naturais. O interesse se justifica, fundamentalmente, na composição química da biomassa, como os carotenoides, devido suas atividades bioativas com efeitos benéficos à saúde. Esses metabólitos por terem uma ótima perspectiva para o crescimento e desenvolvimento industrial, busca-se uma produção com maior potencial através de sistemas de cultivo e condições otimizadas, sendo um desafio subsequente o desenvolvimento de bioprocessos que vinculem os resultados científicos às necessidades comerciais. Nesse contexto, este estudo teve como objetivo analisar o perfil quantitativo de carotenoides produzidos pela microalga Desertifilum spp. em cultivo fototrófico, heterotrófico e mixotrófico. Pelo estudo, constatou-se que a microalga Desertifilum spp. exibe forte potencial para a produção de carotenoides, sendo o cultivo mixotrófico aquele que apresenta melhores resultados quantitativos quando comparado ao cultivo fotoautotrófico e heterotrófico. Dessa forma, o estudo possibilitou demonstrar que as microalgas são fontes de carotenoides, compostos que, segundo a literatura, apresentam importantes propriedades bioativas e/ou nutracêuticas, cuja importância econômica está associada a uma ampla gama de aplicações nas indústrias de alimentos e biomédicas.

References

Alali, M. et al. Nutraceuticals: Transformation of conventional foods into health promoters/disease preventers and safety considerations. Molecules, v.26, n.9, 2540, p.1-28, 2021. https://doi.org/10.3390/molecules2609254

Albrecht, M. et al. Novel hydroxycarotenoids with improved antioxidative properties produced by gene combination in Escherichia coli. Nature Biotechnology, v.18, n.8, p.843–846, 2000. doi:10.1038/78443

Bhat, A.H. et al. Oxidative stress, mitochondrial dysfunction and neurodegenerative diseases; a mechanistic insight. Biomedicine & Pharmacotherapy, v.74, p.101-110, 2015. doi: 10.1016/j.biopha.2015.07.025

Bilal, M. et al. High-value compounds from microalgae with industrial exploitability – a review. Frontiers In Bioscience, Scholar. v.9, n.3, p.319–342, 2017. doi: 10.2741/s490

Borowiak, D. et al. Novel, automated, semi-industrial modular photobioreactor system for cultivation of demanding microalgae that produce fine chemicals - The next story of H. pluvialis and astaxanthin. Algal Research, v.53, 102151, p.1-11, 2021. https://doi.org/10.1016/j.algal.2020.102151

Britton, G.; Liaaen-Jensen, S. & Pfander, H. (Eds.). Carotenoids: Natural Functions. Badel: Birkhäuser Verlag, 2008. 399p. doi: 10.1007/978-3-7643-7499-0

Cheirsilp, B. & Torpee, S. Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresource Technology, v.110, p.510–516, 2012. https://doi.org/10.1016/j.biortech.2012.01.125

Chen, H.; Wang, X. & Wang, Q. Microalgal biofuels in China: The past, progress and prospects. Global Change Biology - GCB Bioenergy. p.1-22, 2020. https://doi.org/10.1111/gcbb.12741

Couch, S.C. et al. Association between long chain polyunsaturated fatty acids and cardiovascular lipid risk factors in you thwith type 1 diabetes: Search Nutrition Ancillary Study. Journal of Diabetes and it Complications, v.31, n.1, p.67-73, 2017. https://doi.org/10.1016/j.jdiacomp.2016.10.002

Deprá, M.C. et al. A new hybrid photobioreactor design for microalgae culture. Chemical Engineering Research and Design, v.144, p.1-10, 2019. doi:10.1016/j.cherd.2019.01.023

Eisenhauer, B. et al. Lutein and zeaxanthin - food sources, bioavailability and dietary variety in age-related macular degeneration protection. Nutrients, v.9, n.2, 120, p.1-14, 2017. doi:10.3390/nu9020120

Fernandes, A.S. et al. HPLC-PDA-MS/MS as a strategy to characterize and quantify natural pigments from microalgae. Current Research in Food Science, v.3, p.100-112, 2020a. doi:10.1016/j.crfs.2020.03.009

Fernandes, A.S. et al. Chlorophylls as food additives. In: Jacob-Lopes, E.; Queiroz, M., Zepka, L. (eds) Pigments from Microalgae Handbook. Springer, Cham, 2020b. https://doi.org/10.1007/978-3-030-50971-2_16

Fernández, L.C. et al. Dietary fats and cardiovascular health. Atención Primaria, v.43, p.1-16, 2011.

Ferreira, A. et al. Future perspectives of microalgae in the food industry. Cultured Microalgae for the Food Industry: Current and Potential Applications. Academic Press, p.387-433, 2021. https://doi.org/10.1016/B978-0-12-821080-2.00008-3

García-Blanco, A. et al. Potential oxidative stress biomarkers of mild cognitive impairment dueto Alzheimer disease. Journal of the Neurological Sciences, v.373, n.15, p.295-302, 2017. https://doi.org/10.1016/j.jns.2017.01.020

Gille, A. et al. Microalgae as a potential source of carotenoids: Comparative results of an in vitro digestion method and a feeding experiment with C57BL/6J mice. Journal of Functional Foods, v.49, p.285-94, 2018.

Gong, M. & Bassi, A. Carotenoids from microalgae: A review of recent developments. Biotechnology Advances, v.34, n.8, p.1396–1412, 2016. doi:10.1016/j.biotechadv.2016.10.005

Gong, Y. & Huang, J. Characterization of four untapped microalgae for the production of lipids and carotenoids. Algal Research, v.49, 101897, 2020. https://doi.org/10.1016/j.algal.2020.101897

Guedes, A.C.; Amaro, H.M. & Malcata, F.X. Microalgae as Sources of Carotenoids. Marine Drugs, v.9, n.4, p.625-644, 2011. https://doi.org/10.3390/md9040625

Gul, K. et al. Chemistry, encapsulation, and health benefits of β-carotene - A review. Cogent Food & Agriculture, v.1, n.1, 2015. doi:10.1080/23311932.2015.1018696

Hamidi, M. et al. Marine bacteria versus microalgae: Who is the best for biotechnological production of bioactive compounds with antioxidant properties and other biological applications? Marine Drugs, v.18, n.1, p.1-38, 2020. doi:10.3390/md18010028

Irazusta, V. et al. Relationship among carotenoid production, copper bioremediation and oxidative stress in Rhodotorula mucilaginosa RCL-11. Process Biochemistry, v.48, p.803–809, 2013. https://doi.org/10.1016/j.procbio.2013.04.006

Jacob-Lopes, E. et al. Bioactive food compounds from microalgae: An innovative framework on industrial biorefineries. Current Opinion in Food Science. v.25, p.1-7, 2019. doi:10.1016/j.cofs.2018.12.003

Jomova, K & Valko, M. Health protective effects of carotenoids and their interactions with other biological antioxidants. European Journal of Medicinal Chemistry, v.70, p.102-110, 2013. https://doi.org/10.1016/j.ejmech.2013.09.054

Khan, M.I.; Shin, J.H. & Kim, J.D. The promising future of microalgae: current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microbial Cell Factories, v.17, n.36, p.1-21, 2018. https://doi.org/10.1186/s12934-018-0879-x

Klassen, J.L. & Foght, J.M. Characterization of Hymenobacter isolates from Victoria Upper Glacier, Antarctica reveals five new species and substantial non-vertical evolution within this genus. Extremophiles, v.15, n.1, p.45-57, 2011. doi: 10.1007/s00792-010-0336-1

Koushan, K. et al. The role of lutein in eye-related disease. Nutrients, v.5, n.5, p.1823-1839, 2013. doi:10.3390/nu5051823

Lafarga, T.; Clemente, I.; Garcia-Vaquero, M. Carotenoids from microalgae. In Carotenoids: Properties, Processing and Applications, ed. C. M. Galanakis, Academic Press, p.149-87, 2020.

Lin, T.-S. & Wu, J.-Y. Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition. Bioresource Technology, v.184, p.100–107, 2015.

Magosso, M.F. et al. Acrocomia aculeata prevents toxicogenetic damage caused by the antitumor agent cyclophosphamide. Genetics and Molecular Research, v.15, n.2, p.1-14, 2016. doi:10.4238/gmr.15027816

Maldonade, I.R.; Rodriguez-Amaya, D.B. & Scamparini, A.R.P. Carotenoids of yeasts isolated from the Brazilian ecosystem. Food Chemistry, v.107, n.1, p.145–150, 2008. https://doi.org/10.1016/j.foodchem.2007.07.075

Mata, T.M.; Martins, A.A. & Caetano, N.S. Microalgae for biodiesel production and other applications: A review. Renewable and Sustainable Energy Reviews, v.14, n.1, p.217–232, 2010. doi:10.1016/j.rser.2009.07.020

Matos, J. et al. Microalgae as healthy ingredients for functional food: a review. Food & Function, v.8, n.8, p.2672–2685, 2017. doi:10.1039/c7fo00409e

Molino, A. et al. Recent developments in supercritical fluid extraction of bioactive compounds from microalgae: Role of key parameters, technological achievements and challenges. Journal of CO₂ Utilization, v.36, p.196–209, 2020. https://doi.org/10.1016/j.jcou.2019.11.014

Mutanda, T. et al. Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production. Bioresource Technology, v.102, n.1, p.57-70, 2011. doi: 10.1016/j.biortech.2010.06.077.

Nascimento, T.C. et al. Bioaccessibility and intestinal uptake of carotenoids from microalgae Scenedesmus obliquus. LWT - Food Science and Technology, 110780, p.1-32, 2020. doi:10.1016/j.lwt.2020.110780

Nörnberg, M.L. et al. Carotenoids profile of Desertifilum spp. in mixotrophic conditions. Brazilian Journal of Development. v.7, n.3, p.33017-33029, 2021. doi:10.34117/bjdv7n3-835

Novoveská, L. et al. Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Marine Drugs, v.17, n.11, 640, p.1-21, 2019. doi:10.3390/md17110640

Patias, L.D. et al. Carotenoid profile of three microalgae/cyanobacteria species with peroxyl radical scavenger capacity. Food Research International, v.100, p.260-266, 2017. https://doi.org/10.1016/j.foodres.2017.06.069

Pina, L.C.C. et al. Evaluation of a microalgae cultivation system with a mix of tubular and parallel plate photobioreactors for microalgae biomass production in alternative culture media. Brazilian Journal of Development, v.7, n.4, p.37734-37777, 2021. doi:10.34117/bjdv7n4-304

Rodrigues, D.B. et al. Production of carotenoids from microalgae cultivated using agroindustrial wastes. Food Research International, v.65, p.144–148, 2014.

Rodrigues, D.B. et al. Bioactive pigments from microalgae Phormidium autumnale. Food Research International, v.77, p.273-279, 2015.

Rodriguez-Amaya, D.B. A guide to carotenoid analysis in foods. Washington, DC: OMNI Research, 2001, 71p.

Rodriguez-Amaya. D.B. Status of carotenoid analytical methods and in vitro assays for the assessment of food quality and health effects. Current Opinion in Food Science. v.1, p.56–63, 2015. doi: 10.1016/j.cofs.2014.11.005

Ru, I.T.K. et al. Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts. Applied Phycology, p.1-10, 2020. doi: 10.1080/26388081.2020.1715256

Sabour-Pickett, S. et al. A review of the evidence germane to the putative protective role of the macular carotenoids for age-related macular degeneration. Molecular Nutrition & Food Research, v.56, n.2, p.270-286, 2012. doi 10.1002/mnfr.201100219

Salbitani, G. et al. Use of an immobilised thermostable α-CA (SspCA) for enhancing the metabolic efficiency of the freshwater green microalga Chlorella sorokiniana. Journal of Enzyme Inhibition and Medicinal Chemistry, v.35, n.1, p.913–920, 2020. https://doi.org/10.1080/14756366.2020.1746785

Sathasivam, R. et al. Microalgae metabolites: A rich source for food and medicine. Saudi Journal of Biological Sciences, p.1-46, 2019. doi:10.1016/j.sjbs.2017.11.003

Sathasivam, R.; KI, J.S. A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Marine Drugs, v.16, n.1, p.1-31, 2018. doi: 10.3390/md16010026.

Schwingshackl, L.; Hoffmann, G. Monounsaturated fatty acids and risk of cardiovascular disease: synopsis of the evidence available from systematic reviews and meta-analyses. Nutrients, v.4, p.1989-2007, 2012. doi:10.3390/nu4121989

Singh, R. et al. Uncovering potential applications of cyanobacteria and algal metabolites in biology, agriculture and medicine: current status and future prospects. Frontiers in Microbiology, v.8, n.112, p.42-46, 2017. doi:10.3389/fmicb.2017.00515

Soares, A.T. et al. Analysis of major carotenoids and fatty acid composition of freshwater microalgae. Heliyon, v.5, n.4, p.1-20, 2019. https://doi.org/10.1016/j.heliyon.2019.e01529

Tang, D.Y.Y. et al. Potential utilization of bioproducts from microalgae for the quality enhancement of natural products. Bioresource Technology, v.304, 122997, p.1-11, 2020. doi: https://doi.org/10.1016/j.biortech.2020.122997

Trejo-Solís, C. et al. Multiple molecular and cellular mechanisms of action of lycopene in cancer inhibition. Evidence-Based Complementary and Alternative Medicine, p.1-17, 2013. doi: 10.1155/2013/705121

Tripathi, M.K. Effect of nutrition on production, composition, fatty acids and nutraceutical properties of milk. Advances in Dairy Research, v.2, p.1‑11, 2014. doi: 10.4172/2329‑888X.1000115

Uenojo, M.; Maróstica Junior, M.R.; Pastore, G.M. Carotenoids: propriedades, aplicações e biotransformação para formação de compostos de aroma. Química Nova, v.30, n.3, p.616-622, 2007. https://doi.org/10.1590/S0100-40422007000300022

Verma, R. et al. Photoautotrophic, mixotrophic, and heterotrophic culture media optimization for enhanced microalgae production. Journal of Environmental Chemical Engineering, 104149, p.1-36, 2020. doi:10.1016/j.jece.2020.104149

Xie, Y. et al. Bioprocess operation strategies with mixotrophy/photoinduction to enhance lutein production of microalga Chlorella sorokiniana FZU60. Bioresource Technology, v.290, 121798, p.1-9, 2019. doi: 10.1016/j.biortech.2019.121798

Zaghdoudi, K. et al. Accelerated solvent extraction of carotenoids from: Tunisian Kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.). Food Chemistry, v.184, p.131-139, 2015. doi: 10.1016/j.foodchem.2015.03.072

Zhang, Z. et al. Elucidating temperature on mixotrophic cultivation of a Chlorella vulgaris strain: different carbon source application and enzyme activity revelation. Bioresource Technology, 123721. 2020. doi: 10.1016/j.biortech.2020.123721

Zhang, Z. et al. The synergistic energy and carbon metabolism under mixotrophic cultivation reveals the coordination between photosynthesis and aerobic respiration in Chlorella zofingiensis. Algal Research, v.25, p.109–116, 2017. https://doi.org/10.1016/j.algal.2017.05.007

Zhang, J. et al. Microalgal carotenoids: beneficial effects and potential in human health. Food & Function, v.5, n.3, p.413-425, 2014. doi:10.1039/c3fo60607d

Published

2022-02-09

How to Cite

Nörnberg, M. L., Pinheiro, P. N., Nascimento, T. C. do, Fernandes, A. S., Lopes, E. J., & Zepka, L. Q. (2022). Produção de biocompostos microalgais em diferentes condições de cultivo / Production of microalgae biocompounds in different cultivation conditions. Brazilian Journal of Development, 8(2), 10226–10240. https://doi.org/10.34117/bjdv8n2-119

Issue

Section

Original Papers