Bactericide and antimicrobial effects of ethanolic extracts of propolis and geopropolis of stingless bees natives from the state of Paraná in multi-resistant microorganisms / Efeitos bactericidas e antimicrobianos de extratos alcoólicos de própolis e geoprópolis de abelhas sem ferrão, nativas do estado do Paraná, em microorganismos multi-resistentes
DOI:
https://doi.org/10.34117/bjdv8n1-191Keywords:
Sustainability, Bee Glue, Microbial resistance, Water resources, Environmental management.Abstract
The value of ecosystem concerning for the maintenance of riparian forests for the protection of water bodies can be essential to the preservation of natural resources, fauna and flora, and all of them inter-connected. Native stingless bees are fundamental pollinating agents for food production, besides the production of propolis and geopropolis, being each of these products, with local characteristics, depending on the vegetation available. The preservation of riparian forests enhances the insertion of meliponaries in communities bordering superficial water bodies and emphasizes the socio-environmental importance because this activity can be an extra source of income for small rural producers. In this work, the antimicrobial and antibacterial potential of propolis from stingless bees, native from the State of Paraná - Brazil, was examined, whose products can be extremely important considering its socio-economic and environmental view. Ethanolic extracts of propolis and geopropolis of native bees were tested according the antimicrobial and bactericidal capacity. The test was conducted with ethanolic extract of Africanized bees, in order to use it as control. The ethanolic extract of propolis and geopropolis of tested species showed to be effective as bactericide for the strains of bacteria Pseudomonas aeruginosa (gram-positive) and Klebsiella planticola, Escherichia coli, Raoutella planticola (gram-negative). In addition, it was observed that fungi can be inertized in honey by reducing the moisture content and the chemical defense barrier due to the substances present in the propolis and geopropolis against microorganisms. Inside the hives bees use propolis/geopropolis as sanitizer, to promote the fixation of microorganisms. In addition to pollination, the hives have an important function as controlling pests and pathogens.
References
APHA, AWWA, WEF in: L.S. Clesceri, A. E.; Greenberg, A. D. Eaton (Eds.), Standard Methods for the Examination of Water and Wastewater, 23th ed., Method 9225 C (Identification), American Public Health Association, American Water Works Association, Water Environment Federation,Washington, DC, USA, 2017.
Brodkiewicz, Y., Marcinkevicius, K., Reynoso, M., Salomon, V., Maldonado, L., Vera, N. (2018) Studies of the biological and therapeutic effects of argentine stingless bee propolis. Journal of drug delivery and therapeutics. v. 8, n. 5, p. 382-392, 2018. DOI: <http://dx.doi.org/10.22270/jddt.v8i5.1889>.
Camargo, J. M. F.; Roubik, D. W. Systematics and bionomics of the apoid obligate necrophages: the trigona hypogea group (Hymnoptera: Apidae; Meliponinae). Biological Journal of Linnean Society. V. 44, n. 1, p. 13-39, 1991. DOI: https://doi.org/10.1111/j.1095-8312.1991.tb00604.x.
Camargo, R. C. R.; Oliveira, K. L.; Berto, M. I. Stingless bee honey: Technical regulation proposal. Brazilian jornal of food technology. V. 20, e 2016157, 2017.
Castaldo, S. Capasso, S. Própolis, an old remedy used in modern medicine. Fitoterapia, v. 73, supl. 1, 2002. DOI: <https://doi.org/10.1016/S0367-326X(02)00185-5>.
Conama - National Council on the Environment. Resolution 420/2009. Establishes the maximum values allowed in soil and sediments. Available in: http://www2.mma.gov.br/port/conama/legiabre.cfm?codlegi=620. Acessed in 30 mar. 2021.
Curitiba, Honey Gardens. Available in: <https://www.curitiba.pr.gov.br/conteudo/jardins-de-mel/2944>. Acess in jun. 2020.
Dastgir, M. G.; Ferreira, F. C.; Peeva, L. G.; Livingston, A. G. Recovery of 2,4-dichlorophenol from acid aqueous streams by membrane aromatic recovery system (MARS). Journal of chemical technology and biotechnology. V. 79, p. 381-390. DOI:< 10.1002/jctb.993>.
Davies, N.; Edwards, P. A.; Lawrence, M. A. M.; Simkiss, K.; Taylor, M. G. Biocide testing using particles with controlled surface properties (artificial sediments). Environmental Chemistry. v. 18, n. 10, p.2337-2342, 2009. DOI: https://doi.org/10.1002/etc.5620181030.
Dimmock, J. R.; Elias, D. W.; Beazely, M. A.; Kandepu, N. M. Bioactivitis of Chalcones. Current Medicinal Chemistry, v. 6 p. 1125-1149, 1999.
Ferreira, J. M.; Silva, C. C. F.; Salatino, A.; Message, D.; Negri, G. Antioxidant activity of a geopropolis from northeast Brazil chemical characterization and likely botanical origin. Evidence-based complementary and althernative medicine. 2017. DOI: <https://doi.org/10.1155/2017/4024721>.
Fiege, H. Cresols and Xylenols. In Ullmann’s Enciclopedia of industrial chemistry. 2012. DOI: 10.1002/14356007.a08_025.
Fischer, G.; Hübner, S. O.; Vargas, G. D.; Vidor, T. Immunomodulation induced by propolis. Arquivos do Instituto Biológico, v. 75, n. 2, p. 247-253, 2008
Freitas, B. M. 2006. Pollinators and pollination: the economic value of conservation (in portuguese). Available in: http://www.reacao.com.br/programa_ sbpc57ra/sbpccontrole/textos/brenofreitas.htm Acess in: 17 jan. 2021.
Ghisalberti, E. L. Propolis, a review. Bee World, v. 60, p. 59-84, 1979. DOI: <https://doi.org/10.1080/0005772X.1979.11097738>.
Grosso, G. S.; Tangarife, M. P. O. Capítulo 10. Propóleos. Actividad biológica. In.: Grosso, G. S. Origen, Naturaleza, propiedades fisicoquímicas y valor terapéutico del propóleo. Universidad de Tolima. Colômbia, 2017
Itaipu, Project Preserving Good Water (2020). Available at: <https://www.itaipu.gov.br/meioambiente/cultivando-agua-boa>. Acessesd June, 2020.
Kozalec, I.; Pepeljnjak, S.; Bakmaz, M.; Knežević, S. Flavonoid analysis and antimicrobial activity of commercially available prpopolis product. Acta Pharm. V. 55, n. 4, p. 423-430, 2005. DOI: <10.2147/IJN.S219577>.
Lalonde, S.; Wipf, D.; Frommer, W. B. Transport mechanism for organic forms of carbon and nitrogen between source and sink. Annual review of plant biology. V. 55, p. 341-372, 2004.
Magiorakos, A. P.; Srinivasan, A.; Carey, R. B.; Carmeli, Y.; Falagas, M. E.; Giske, C. G.; Harbarth, S.; Hindler, J. F.; Kahlmeter, G.; Olsson-lijequist, B.; Paterson, D. L.; Rice, L. B.; Stelling, J.; Struelens, M. J.; Vatopoulos, A.; Weber, J. T.; Monnet, D. L. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquire resistance. Bacteriology. n. 18, p. 268-281, 2011. DOI: 10.1111/j.1469-0691.2011.03570.x.
Moraes, G. K. A.; Ferraz, L. F.; Chapla, V. M. Volatile organic compounds of endophytic fungi and biotechnological applications. Revista virtual de química. V. 12, n 6, p. 1498-1510, 2020. DOI: 10.21577/1984-6835.20200116.
Namany, S.; Al-ansari, T.; Govindan, R. Sustainable energy, water and food nexus systems: a focused review of decision-making tools for efficient resource management and governance. Journal of Clean Production, v. 225, p. 610-626, 2019. DOI: https://doi.org/10.1016/j.jclepro.2019.03.304.
Nascimento, W. M.; Gomes, E. M. L.; Batista, E. A.; Freitas, R. A. Influence of pollinators on seed production and quality of carrot and sweet pepper in a greenhouse Horticultura Brasileira, N. 30, P. 494-498. 2012.
Negri, G.; Silva, C. C. F.; Coelho, G. R.; Nascimento, R. M.; Mendonça, R. Z. Cardanols detected in non-polar própolis extracts from Scaptotrigona aff. Postiça (Hymnoptera, Apidae, Meliponini). Brazilian jornal of food technology, 2019. DOI: <https://doi.org/10.1590/1981-6723.26518>.
Orr, M. C.; Hugens, A. C.; Chesters, D.; Pickering, J.; Zhu, C. D.; Ascher, J. S. Global patterns and drivers of bee distribution. Current Biology. 2020. DOI: <https://doi.org/10.1016/j.cub.2020.10.053>.
Orsi, R. O.; Funari, S. R. C.; Soares, A. M. V. C.; Calvi, S. A.; Oliveira, S. L.; Sforcin, J. M.; Bankova, V. Immunomodulatory action of propolis on macrophage activation. The Journal of Venomous Animals and Toxins. v. 6, n. 2, p. 205-219, 2000.
Park, Y. K.; Ikegaki, M.; Alencar, S. M. Classification of Brazilian propolis from its physico-chemical characteristics and biological properties. Sweet message, São Paulo, n. 58, 2000.
Petri, J. L.; Haverroth, F. J.; Leite, G. B.; Sezerino, A. A.; Couto, M. Growth regulators for temperate climate fruit. Florianópolis: Epagri, 2016, 141p.
Radman, R.; Saez, T.; Bucke, C.; Keshavarz. Elicitation of plants and microbial cell systems. Biotechnolgy and applied biochemistry. V. 37, n. 1, p. 91-102, 2010. DOI: <10.1042/ba20020118>.
Resende, J. D. Photodegradation of 2,4-dichlorophenol in aqueous solution. Dissertation, University of São Paulo, Brazil, 2011.
Silva, S. F.; Ferreira-Romanichen, F. M.D.; Antonelli-Ushirobira, T. M. Antimicrobial activity in vitro of punica granatum on gram-negative and gram-positive bacteria. Brazilian Journal of Development, v. 7, n. 11, 2021. DOI: <10.34117/bjdv7n11-196>.
Silveira, F. A.; Melo, G. A. R.; Almeida, E. A. B. Brazilian bees - systematic and identification. Belo Horizonte. 2002. 253p.
Šturm, L.; Ulrih, N. P. Chapter two - Propolis flavonoids and terpenes, and their interactions with model lipid membranes: a review. In: Advances in biomembranes and lipid self-assembly, v. 32, p. 25-52. 2020. DOI: <https://doi.org/10.1016/bs.abl.2020.04.003>.
Yusof, N.; Munaim, M. S. A.; Kutty, R. V. The effects of diferente etanol concentration on total phenolic and total flavonoid contente in Malaysian própolis. Materials Science and Engineering 991, 2020. DOI: <10.1088/1757-899X/991/1/012033>.
