Effect of commercial extract of citrus aurantium in obese rats induced by cafeteria diet/ Efeito do extrato comercial de citrus aurantium em ratos obesos induzidos por dieta de cafeteria
DOI:
https://doi.org/10.34117/bjdv5n10-134Keywords:
Dyslipidemias. Oxidative stress. Bitter orange. Weight loss. Synephrine.Abstract
Obesity is the result of a higher caloric energy expenditure increasing the risk of developing diabetes and cardiovascular diseases. Citrus aurantium, has been used for weight reduction by having p-Synephrine, a compound associated with this action. The objective of this work was to evaluate the effects of commercial extract of Citrus aurantium in weight alteration in the biochemical parameters of rats induced to obesity by High fat / cafeteria diet. The animals were divided into four experimental groups: Group 1: Standard commercial feed; Group 2: standard commercial feed, and solution of commercial extract of Citrus aurantium, via gavage; Group 3: high fat/cafeteria feed. Group 4: high fat/cafeteria feed commercial extract of Citrus aurantium, via gavage. In order to maintain the same experimental conditions in all groups, the animals in groups 1 and 3, received filtered water via gavage. Tests were performed to evaluate the antioxidant capacity of commercial extract of Citrus aurantium: total phenolic compounds, DPPH and FRAP.E in the plasma of animals: FRAP in addition to analyzes of blood biochemical parameters. Results obtained with the commercial extract showed positive results, however, the antioxidant activity in plasma, was not able to alleviate oxidative stress. Significant differences were found in the values of Aspartate aminotransferase, alanine aminotransferase, VLDL cholesterol, HDL and triglycerides, however there was no reduction of weight. The commercial extract of Citrus aurantium, is important in the prevention of hepatic damage and diseases linked to elevations in the levels of VLDL and triglyceride levels, especially when this increase is associated with obesity.
References
Peixoto MDRG, Benício MD, Latorre MRDO, Jardim PCBV. Circunferência da cintura e índice de massa corporal como preditores da hipertensão arterial. Arq bras cardiol, 2006; 87(4): 462-70.
World Health Organization. Obesity: preventing and managing the global epidemic. World Health Organization: n. 894; 2000.
Lessa I. O adulto brasileiro e as doenças da modernidade: epidemiologia das doenças crônicas não-transmissíveis. Editora Hucitec: Vol. 114; 1998.
Pinheiro ARDO, Freitas SFTD, & Corso ACT. Uma abordagem epidemiológica da obesidade. Revista de Nutrição, 2004; 17(4): 523-533.
França BK, Alves MRM, Souto FMS, Tiziane L, Boaventura RF, Guimarães A, Alves JrA. Peroxidação lipídica e obesidade: Métodos para aferição do estresse oxidativo em obesos. GE jornal português de gastrenterologia. 2013; 20(5): 199-206.
Muthiah PL. In vitro antioxidant activities of leaves, fruits and peel extracts of Citrus. International Journal of Phytopharmacy. 2012; 2(1): 13-20.
Fu L, Xu BT, Xu XR, Gan R Y, Zhang Y, Xia EQ, et al. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chemistry. 2011: 129(2); 345-350.
Tardido AP, Falcão MC. O impacto da modernização na transição nutricional e obesidade. Rev Bras Nutr Clin, 2006; 21(2): 117-24.
Popkin BM, Doak CM. The obesity epidemic is a worldwide phenomenon. Nutrition reviews, 1998; 56(4): 106-114.
OMS - Organización Mundial de la Salud. Obesidad y sobrepeso. Nota descriptiva N° 311, 2015. Disponível em: http://www.who.int/mediacentre/factsheets/fs311/es/. Acesso: 27 jul. 2016.
ABESO. Diretrizes brasileira de obesidade 2009/2010. 3.ed. - Itapevi: AC Farmacêutica, 2009.
Carvalho EAA, Simão MTJ, Fonseca MC, Andrade RG, Ferreira MSG, Silva AF. Obesity: epidemiological aspects and prevention. Rev Medi Minas Gerais, 2013; 23(1): 72-80.
Sjöström L, Narbro K, Sjöström CD, Karason K, Larsson B, Wedel H, Bengtsson C. Effects of bariatric surgery on mortality in Swedish obese subjects. New England journal of medicine, 2007; 357(8): 741-752.
Pinto, DCM. A Fitoterapia no tratamento da obesidade [Tese]. Porto: Faculdade de Ciências da Saúde, Universidade Fernando Pessoa; 2013.
Sanguinetti, E. E. Plantas que curam. Porto Alegre: Rígel; 1989.
DCM, T. (2009). Suplementos alimentares e redução de peso. Universidade do Porto. Faculdade de Ciências da Nutrição e Alimentação, 14-15.
Dwyer JT, Allison DB, Coates PM. Dietary supplements in weight reduction. Journal of the Academy of Nutrition and Dietetics, 2005; 105(5): 80-86.
Haaz S, Fontaine KR, Cutter G, Limdi N, Perumean?Chaney S, Allison DB. Citrus aurantium and synephrine alkaloids in the treatment of overweight and obesity: an update. Obesity reviews. 2006; 7(1): 79-88.
ARBO, MD. Avaliação toxicológica de p-sinefrina e extrato de Citrus aurantium L. (rutaceae) [dissertação]. Porto Alegre: Universidade Federal do Rio Grande do Sul; 2008.
Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965; 16: 144-158.
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006; 19: 669-675.
Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chemistry. 2006; 99: 381-387.
Pulido R, Bravo, L Saura-Calixto, F. Antioxidant Activity of Dietary Polyphenols As Determined by a Modified Ferric Reducing/Antioxidant Power Assay, J. Agric. Food Chem. 2000; 48: 3396-3402.
Benzie FFI, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay, Analytical biochemistry. 1996; 239: 70-76
Jones D. Regulation of appetite, body weight and athletic function with materials derived from citrus varieties. U.S. Patent. 2001; 6: 224, 873
Verrengia EC, Kinoshita SAT, Amadei JL. Medicamentos fitoterápicos no tratamento da obesidade. Uniciências. 2015; 17(1): 53-58.
Turchetto, Q, Andreato L, Almeida FN, Esteves JVDC, de Moraes SMF. Suplementação de ácido linoléico conjugado associado ao exercício físico em parâmetros morfofuncionais de ratos submetidos à dieta de cafeteria. Journal of Physical Education. 2013; 24(1): 121-134.
Macedo IC, Medeiros L F, Oliveira C, Oliveira, CM, Rozisky JR, Scarabelot VL, Caumo W. Cafeteria diet-induced obesity plus chronic stress alter serum leptin levels. Peptides. 2012; 38(1): 189-196.
Fontes GG. Efeito de formulação fitoterápica contendo Calendula officinalis no metabolismo lipídico em ratos Wistar alimentados com dieta de cafeteria [Dissertação]. Viçosa: Universidade Federal de Viçosa; 2015
Rossi-Jesus D, Barbosa LN, Prando TBL, Martins LF, Gasparoto F, Guedes KMR, et al. Ninety-day oral toxicity assessment of an alternative biopolymer for controlled release drug delivery systems obtained from cassava starch acetate. Evidence-Based Complementary and Alternative Medicine. 2015; 2015: 1-7.
AYRES M, AYRES JrM, AYRES DL, SANTOS AAS. Bioestat 5.0 aplicações estatísticas nas áreas das ciências biológicas e médicas. Sociedade Civil de Mamirauá: 2007, 364p.
Ross SA, Ziska DS, Zhao K, ElSohly MA. Variance of common flavonoids by brand of grapefruit juice. Fitoterapia. 2000; 71(2): 154-161.
Dadkhah A, Fatemi F, Kazemnejad S, Rasmi Y, Ashrafi-Helan J, Allameh A. Differential effects of acetaminophen on enzymatic and non-enzymatic antioxidant factors and plasma total antioxidant capacity in developing and adult rats. Molecular and cellular Biochemistry. 2006;281(1-2); 145-152.
Kumari A, Kakkar P. Lupeol prevents acetaminophen-induced in vivo hepatotoxicity by altering the Bax/Bcl-2 and oxidative stress-mediated mitochondrial signaling cascade. Life sciences. 2012; 90(15-16): 561-570.
Gonçalves GA, Soares AA, Correa RC, Barros L, Haminiuk CW, Peralta R M, Bracht A. Merlot grape pomace hydroalcoholic extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritis. Journal of Functional Foods. 2017; 33: 408-418.
Marzouk B. Characterization of bioactive compounds in Tunisian bitter orange (Citrus aurantium L.) peel and juice and determination of their antioxidant activities. BioMed research international. 2013; 2013: 1-12
Karimi E, Oskoueian E, Hendra R, Oskoueian A, Jaafar HZ. Phenolic compounds characterization and biological activities of Citrus aurantium bloom. Molecules. 2012; 17(2): 1203-1218.
Kennedy GC. The role of depot fat in the hypothalamic control of food intake in the rat. In Proc. R. Soc. Lond. 1953; 140 (901); 578-592.
Woods S C, Seeley RJ, Rushing PA, D'Alessio D, Tso P. A controlled high-fat diet induces an obese syndrome in rats. The Journal of nutrition. 2003; 133 (4): 1081-1087.
Almeida MFSD, Marcellino MCL, Nicolielo DB, Pedro KP, Neves FTA, Moura KCRD, Gonçalves TDOM. Avaliação do potencial termogênico e do perfil bioquímico de camundongos suíços submetidos ao uso diário do extrato aquoso do citrus aurantium L. Salusvita. 2015; 34(3): 489-504.
Verpeut JL, Walters AL, Bello NT. Citrus aurantium and Rhodiola rosea in combination reduce visceral white adipose tissue and increase hypothalamic norepinephrine in a rat model of diet-induced obesity. Nutrition research. 2013: 33(6); 503-512.
Arbo MD, Schmitt GC, Limberger MF, Charão MF, Moro ÂM, Ribeiro GL, et al. Subchronic toxicity of Citrus aurantium L.(Rutaceae) extract and p-synephrine in mice. Regulatory Toxicology and Pharmacology. 2009; 54(2): 114-117.
Arbo, MD, Franco MT, Larentis ER, Garcia SC, Sebben VC, Leal MB et al. Screening for in vivo (anti) estrogenic activity of ephedrine and p-synephrine and their natural sources Ephedra sinica Stapf. (Ephedraceae) and Citrus aurantium L.(Rutaceae) in rats. Archives of toxicology. 2009; 83(1): 95-99.
Stohs SJ. Safety, Efficacy, and Mechanistic Studies Regarding Citrus aurantium (Bitter Orange) Extract and p?Synephrine. Phytotherapy Research. 2017; 31: 1463-1474.
Kaats GR, Miller H, Preuss HG, Stohs SJ. A 60 day double-blind, placebo-controlled safety study involving Citrus aurantium (bitter orange) extract. Food and Chemical Toxicology. 2013; 55: 358-362.
Marli S, Adroaldo L. Influência da dieta na concentração sérica de triglicerídeos. Jornal Brasileiro de Patologia e Medicina Laboratorial. 2003; 39(4): 283-288.
Sharma M, Fernandes J, Ahirwar D, Jain R. Hypoglycemic and hypolipidimic activity of alcoholic extract of citrus aurantium in normal and alloxan-induced diabetic rats. Pharmacology. 2008; 3: 161-171.
Sagae SC, Lubaczeuski C, Zacharias P, Bonfleur mL, Franci CR, Sanvitto G L. Prevention of metabolic disorders and reproductive performance deficits by the blockade of Angiotensin II AT1 receptor in female rats fed with cafeteria diet. Physiology & behavior. 2013; 119: 1-8.
Moraes VBD. Efeito do resíduo da moagem a seco de milho micropulverizado no metabolismo lipídico, na glicemia e na composição corporal em ratos alimentados com dieta de cafeteria [dissertação]. Viçosa: Universidade Federal de Viçosa; 2009.
Águila MB, Loureiro CC, Pinheiro ADR, Mandarim-de-Lacerda CA. Metabolismo lipídico de ratos alimentados com diferentes tipos de lipídios. Arq Bras Cardiol. 2002; 78(1): 25-31.
Campelo LML, Sá CG, Feitosa CM, Sousa GF, Freitas R M. Chemical constituents and toxicological studies of the essential oil extracted from Citrus limon Burn (Rutaceae). Revista Brasileira de Plantas Medicinais. 2013; 15(4): 708-716.
