Influência da carga de substrato na esporulação do fungo metarhizium anisopliae em embalagens plásticas / Influence of substrate loading on sporulation of fungus metarhizium anisopliae in plastic packaging
DOI:
https://doi.org/10.34117/bjdv5n6-167Keywords:
Metarhizium anisopliae, Cultivo em Estado Sólido, Esporo, Embalagem Plástica.Abstract
Este trabalho teve como objetivo analisar a influência da carga do arroz tipo 1 na esporulação do fungo Metarhizium anisopliae em embalagens plásticas. Com objetivo de otimizar e gerar economia no processo de produção de esporos do fungo realizou – se teste em embalagens plásticas de polipropileno com diferentes tamanhos comportando diferentes cargas de arroz. Observou – se com os resultados obtidos que é possível produzir esporos em embalagens plásticas com altos níveis de cargas, porém com embalagens apropriadas e de tamanho ideal para fornecer aeração e evitar constipação das embalagens.
References
Almeida, J. E. M., Batista Filho, a., 2001. Banco de Microrganismos Entomopatogênicos. Biotecnol. Ciência e Desenvolv. 30–33.
Alonso-Díaz, M.A., García, L., Galindo-Velasco, E., Lezama-Gutierrez, R., Angel-Sahagún, C.A., Rodríguez-Vivas, R.I., Fragoso-Sánchez, H., 2007. Evaluation of Metarhizium anisopliae (Hyphomycetes) for the control of Boophilus microplus (Acari: Ixodidae) on naturally infested cattle in the Mexican tropics. Vet. Parasitol. 147, 336–340. https://doi.org/10.1016/j.vetpar.2007.03.030
Castro, T., Mayerhofer, J., Enkerli, J., Eilenberg, J., Meyling, N. V., Moral, R. de A., Demétrio, C.G.B., Delalibera, I., 2016. Persistence of Brazilian isolates of the entomopathogenic fungi Metarhizium anisopliae and M. robertsii in strawberry crop soil after soil drench application. Agric. Ecosyst. Environ. 233, 361–369. https://doi.org/10.1016/j.agee.2016.09.031
Dorta, B., Arcas, J., 1998. Sporulation of Metarhizium anisopliae in solid-state fermentation with forced aeration. Enzyme Microb. Technol. 23, 501–505. https://doi.org/10.1016/S0141-0229(98)00079-9
Driver, F., Milner, R.J., Trueman, J.W.H., 2000. A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data. Mycol. Res. 104, 134–150. https://doi.org/10.1017/S0953756299001756
Galindo-Velasco, E., Lezama-Gutiérrez, R., Cruz-Vázquez, C., Pescador-Rubio, A., Angel-Sahagún, C.A., Ojeda-Chi, M.M., Rodríguez-Vivas, R.I., Contreras-Lara, D., 2015. Efficacy of entomopathogenic fungi (Ascomycetes: Hypocreales) against adult Haematobia irritans (Diptera: Muscidae) under stable conditions in the Mexican dry tropics. Vet. Parasitol. 209, 173–178. https://doi.org/10.1016/j.vetpar.2015.02.025
Hu, G., Leger, R., 2002. Field studies using a recombinant mycoinsecticide (Metarhizium anisopliae) reveal that it is rhizosphere competent. Appl. Environ. Microbiol. 68, 6383–6387. https://doi.org/10.1128/AEM.68.12.6383
Jenkins, N.E., Heviefo, G., Langewald, J., J, A., Lomer, C.J., Park, S., Sl, B., 1998. Development of mass production technology for aerial conidia for use as mycopesticides. BioControl 19, 21–32.
Keyser, C.A., Fernandes, É.K.K., Rangel, D.E.N., Roberts, D.W., 2014. Heat-induced post-stress growth delay: A biological trait of many Metarhizium isolates reducing biocontrol efficacy? J. Invertebr. Pathol. 120, 67–73. https://doi.org/10.1016/j.jip.2014.05.008
Lacey, L.A., Grzywacz, D., Shapiro-Ilan, D.I., Frutos, R., Brownbridge, M., Goettel, M.S., 2015. Insect pathogens as biological control agents: Back to the future. J. Invertebr. Pathol. 132, 1–41. https://doi.org/10.1016/j.jip.2015.07.009
Lanza, L.M., Monteiro, A.C., Malheiros, E.B., 2009. Sensibilidade de Metarhizium anisopliae à temperatura e umidade em três tipos de solos. Ciência Rural 39, 6–12. https://doi.org/10.1590/S0103-84782009000100002
Loureiro, E.S., Batista Filho, A., Almeida, J.E.M., Mendes, J.M., Pessoa, L.G.A., 2012. Efficiency of Metarhizium anisopliae (Metsch.) Sorok isolates in the control of the sugarcane spittlebug Mahanarva fimbriolata (Stal, 1854) (Hemiptera: Cercopidae) in field conditions. Arq. Inst. Biol 79, 47–53. https://doi.org/10.1590/S1808-16572012000100007
Nishi, O., Sato, H., 2017. Species diversity of the entomopathogenic fungi Metarhizium anisopliae and M. flavoviride species complexes isolated from insects in Japan. Mycoscience 58, 472–479. https://doi.org/10.1016/j.myc.2017.06.008
Paula, A., Frazzon, G., Da, I., Vaz Junior, S., Masuda, A., Schrank, A., Vainstein, M.H., 2000. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Vet. Parasitol. 94, 117–125. https://doi.org/10.1016/S0304-4017(00)00368-X
Polar, P., De Muro, M.A., Kairo, M.T.K., Moore, D., Pegram, R., John, S.A., Roach-Benn, C., 2005. Thermal characteristics of Metarhizium anisopliae isolates important for the development of biological pesticides for the control of cattle ticks. Vet. Parasitol. 134, 159–167. https://doi.org/10.1016/j.vetpar.2005.07.010
Santos, V., Mascarin, G.M., da Silva Lopes, M., Alves, M.C.D.F., Rezende, J.M., Gatti, M.S.V., Dunlap, C.A., Delalibera Júnior, Í., 2017. Identification of double-stranded RNA viruses in Brazilian strains of Metarhizium anisopliae and their effects on fungal biology and virulence. Plant Gene 11, 49–58. https://doi.org/10.1016/j.plgene.2017.01.001
Schmidt, F.. G.V., Silva, J.B.T., Faria, M.R., Magalhães, B.P., Alves, R.T., Lecoq, M., 2007. Methodology of application of the fungus Metarhizium anisopliae var. acridum for the control of the grasshopper Rhammatocerus schistocercoides in the field. In: Bol [WWW Document]. Bol. Pesqui. e Desenvolv. 208. URL https://www.embrapa.br/web/mobile/publicacoes/-/publicacao/189419/metodologia-de-aplicacao-do-fungo-metarhizium-anisopliae-var-acridum-para-o-controle-do-gafanhoto-rhammatocerus-schistocercoides-em-campo
Shin, T.Y., Bae, S.M., Woo, S.D., 2016. Screening and characterization of antimicrobial substances originated from entomopathogenic fungi. J. Asia. Pac. Entomol. 19, 1053–1059. https://doi.org/10.1016/j.aspen.2016.09.008
Wang, J.B., St. Leger, R.J., Wang, C., 2016. Advances in Genomics of Entomopathogenic Fungi. Adv. Genet. 94, 67–105. https://doi.org/10.1016/bs.adgen.2016.01.002
