Efecto del plasma rico en plaquetas sobre el desarrollo in vitro de embriones bovinos

Autores

  • Alejandro Romero Mercado
  • Rodolfo Canseco Sedano
  • Felipe Montiel Palacios
  • Oscar Enrique Zárate Guevara
  • Gustavo Contreras Hernández
  • Vicente Eliezer Vega-Murillo
  • Itziar Eukene Lepe Anasagasti
  • Adalberto Rosendo Ponce

DOI:

https://doi.org/10.34188/bjaerv9n2-173

Palavras-chave:

blastocisto, embrioquinas, cultivo

Resumo

El desarrollo in vitro de embriones bovinos es subóptimo. El objetivo fue evaluar el efecto de añadir plasma rico en plaquetas (PRP) sobre el desarrollo in vitro de embriones bovinos. El PRP fue obtenido a partir de sangre sometida a dos ciclos de centrifugación y activación con 10% de cloruro de calcio 24 h previo a ser adicionado al medio de cultivo. Se utilizaron 755 complejos-cúmulus ovocito (CCO´s) los cuales fueron sometidos a maduración y fertilización con semen descongelado de un mismo semental con fertilidad probada. Los presuntos cigotos fueron divididos en tres tratamientos: TC= Medio de cultivo sin PRP (n= 246), T 2.5 %= medio de cultivo adicionado con 2.5% de PRP (n= 257), y T 5%= medio de cultivo adicionado con 5% de PRP (n=252). Los datos de desarrollo embrionario fueron analizados utilizando regresión logística. La división celular fue evaluada 48 h posteriores al inicio del cultivo in vitro, obteniendo 64, 66 y 62% respectivamente por tratamiento (P>0.05). El desarrollo a blastocisto fue evaluado los días 7 y 8 de cultivo y comparado con el número de embriones divididos encontrados a las 48 horas pos-fertilización. Los tratamientos adicionados con 2.5 y 5% de PRP (56 y 57% respectivamente) fueron superiores (P<0.05) al tratamiento control (43%). Se concluye que la adición de 2.5 % ó 5% de PRP en el medio de cultivo beneficia el desarrollo de embriones bovinos producidos in vitro.

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Publicado

2026-05-26

Como Citar

Mercado, A. R., Sedano, R. C., Palacios, F. M., Guevara, O. E. Z., Hernández, G. C., Vega-Murillo, V. E., Anasagasti, I. E. L., & Ponce, A. R. (2026). Efecto del plasma rico en plaquetas sobre el desarrollo in vitro de embriones bovinos. Brazilian Journal of Animal and Environmental Research, 9(2), e87330. https://doi.org/10.34188/bjaerv9n2-173

Edição

v. 9 n. 2 (2026)

Seção

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