The inhibitory activity of propoxur on acetylcholinesterase varies among domestic animal species

Autores

  • María Guadalupe Prado-Ochoa
  • Samantha Michel Gómez Castro
  • Ariadna Gabriela Martínez González
  • Manuel Alejandro Hernández Serda
  • Luis Alfonso Cárdenas Granados
  • Víctor Hugo Vázquez-Valadez
  • Marco Antonio Muñoz-Guzmán
  • Enrique Angeles
  • Fernando Alba-Hurtado

DOI:

https://doi.org/10.34188/bjaerv8n4-206

Palavras-chave:

propoxur, acetylcholinesterase, neurotoxicity, molecular docking

Resumo

The aim of this study was to compare in silico (molecular docking) affinity of acetylcholinesterase (AChE) from different objective and non-objective animal species (cattle ticks, honeybees, chickens, rats, humans, rabbits, pigs, sheep, goats, and cattle) to propoxur, and in vitro the inhibition kinetics of AChE activity produced by this compound. Molecular docking showed that the number of amino acids in AChE varied among the species. The catalytic triad (His, Glu, and Ser) was conserved, although its position within the protein varied among species. The lowest similarity in protein identity was between arthropods compared to mammals and chickens. In silico predictions showed that propoxur has a higher affinity energy but lower binding efficiency to AChE than acetylcholine. In vitro kinetic constants (Km and Vmax) showed clear differences in enzyme affinity and activity between species. Furthermore, differences in the inhibition kinetic constants of propoxur on AChE were observed between species. A higher inhibitory power (ki), higher carbamylation rate (k2), lower dissociation rate (kd), and lower IC50 values were observed in porcine AChE inhibited by propoxur compared with those of AChE from the other species, suggesting that pigs have higher susceptibility to propoxur. In contrast, a lower carbamylation rate and higher IC50 value were observed in cattle, suggesting a greater resistance in cattle and differences in vivo susceptibility between non-objective species to propoxur. In silico and in vitro results reveal a differentiated effect of propoxur on AChE activity in different species and its neurotoxic potential.

   

Biografia do Autor

María Guadalupe Prado-Ochoa

Samantha Michel Gómez Castro

Ariadna Gabriela Martínez González

Manuel Alejandro Hernández Serda

Luis Alfonso Cárdenas Granados

Víctor Hugo Vázquez-Valadez

Marco Antonio Muñoz-Guzmán

Enrique Angeles

Fernando Alba-Hurtado

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Publicado

2025-12-21

Como Citar

Prado-Ochoa , M. G., Castro, S. M. G., González, A. G. M., Serda , M. A. H., Granados, L. A. C., Vázquez-Valadez, V. H., Muñoz-Guzmán, M. A., Angeles, E., & Alba-Hurtado, F. (2025). The inhibitory activity of propoxur on acetylcholinesterase varies among domestic animal species. Brazilian Journal of Animal and Environmental Research, 8(4), e84722. https://doi.org/10.34188/bjaerv8n4-206

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v. 8 n. 4 (2025)

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