Sensibilidad al extracto de ajo en Salmonella resistente a antibióticos aislada de gallinas
DOI:
https://doi.org/10.34188/bjaerv8n4-161Palavras-chave:
Salmonella, galinhas, antibiótico, resistência, alho, sensibilidadeResumo
As galinhas são um dos principais reservatórios de Salmonella, e esta zoonose é a principal causa de gastroenterite de origem alimentar em todo o mundo. O objetivo foi determinar a sensibilidade do extrato de alho à Salmonella resistente a antibióticos isolada de fezes de frango. O isolamento foi realizado utilizando água peptonada, caldo rapapport vasiliadis e ágar entérico Hektoen. Os testes de inibição foram realizados em colônia previamente identificada por coloração de Gram, testes bioquímicos e PCR (amplificação do fragmento do gene InvA). O perfil de resistência aos antibióticos e a sensibilidade bacteriana ao extrato de alho foram determinados por disco-difusão. Salmonella foi identificada por coloração de Gram, exames bioquímicos e banda amplificada de 285 pb com frequência de 28/70 (40%). A cepa apresentou resistência a múltiplas drogas (sulfonamidas, penicilinas e anfenicóis), e o alho inibiu seu crescimento em nível semelhante à cefotaxima e igual à amicacina (controles). Isto sugere que o extrato de alho pode ser uma alternativa aos antibióticos para controlar Salmonella spp. em galinhas produtoras de ovos.
Referências
ABOU ELEZ RM, Elsohaby I, El Gazzar N, Tolba HMN, Abdelfatah EN, Abdellatif SS, Mesalam AA, Tahoun ABM. 2021. Antimicrobial resistance of Salmonella enteritidis and Salmonella typhimurium isolated from laying hens, table eggs and humans with respect to antimicrobial activity of biosynthesized silver nanoparticles. Animals. 11(12):3554. ISSN: 2076-2615. https://doi.org/10.3390/ani11123554
AGBAJE M, Awosile B, Kehinde OO, Omoshaba EO, Dipeolu M, Bankole NO. 2021. Diverse non-typhoidal Salmonella serovars with multi-drug resistance potentials isolated from chicken faeces in Ogun State, Nigeria. Sokoto Journal of Veterinary Sciences. 19(2):95-105. P-ISSN 1595-093X: E-ISSN 2315-6201. http://dx.doi.org/10.4314/sokjvs.v19i2.4
AL-ABADI IKM, Al- Mayah AAS. 2011. Isolation and identification of Salmonella spp. from chicken and chicken environment in Basrah province. African J. Biol. Sci. 7(1):33-43. ISSN 1687-4870. https://scholar.google.es/scholar?hl=es&as_sdt=0%2C5&q=Isolation+and+identification+of+Salmonella+spp.+from+chicken+and+chicken+environment+in+Basrah+province+I.+K.+M.+Al-Abadi%2C+A.+Mayah&btnG=
ALVES A, Santos-Ferreira N, Magalhaes R, Ferreira V, Teixeira P. 2022. From chicken to salad: Cooking salt as a potential vehicle of Salmonella spp. and Listeria monocytogenes cross-contamination. Food Control. 137: 108959. ISSN: 0956-7135. http://Doi.org/10.1016/j.foodcont.2022.108959
AMADO OC. 2023. Historia, clasificación taxonómica, características microbiológicas y patogénesis de Salmonella spp: Revisión sistemática. [Tesis de licenciatura, Universidad de Antioquía, Escuela de Microbiología]. https://bibliotecadigital.udea.edu.co/dspace/bitstream/10495/40064/1/AmadoCatalina_2023_SalmonellaPatogénesisClasificación.pdf
AMIN HS, Abdelrahman AA, Abdellrazeq GS. 2015. Occurrence of multidrug Resistant Salmonella Enterica in retail chicken meat and development of a six genes based multiplex PCR as an alternative diagnostic method. Journal of Food Safety. 36:459-466. O-ISSN:1745-4565. P-ISSN:0149-6085. https://doi.org/10.1111/jfs.12260
BAGDE SB, Mondal R, Naidu SBK, Khatoon JA, Govender P, Anupam R. 2021. Antibacterial properties of organosulfur compounds of garlic (Allium sativum). Frontiers in Microbiology. 12(613077):1-20. ISSN:1664-302X. https://Doi:10.3389/fmicb.2021.613077
BENKEBLIA N. 2004. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). Lebensmittel Wissenschaft Technologie. 37: 263–268. P-ISSN: 0023-6438. O-ISSN: 1096-1127. https://Doi:10.1016/j.lwt.2003.09.001
BRENNER FW, Villar RG, Angulo FJ, Tauxe R, Swaminathan B. 2000. Salmonella nomenclature. Journal of Clinical Microbiology. 38(7):2465–2467. L-ISSN: 2995-8539. https://DOI:10.1128/JCM.38.7.2465-2467.2000
BORGES KA, Furian TQ, Souza SN, Salle CTP, Moraes HLS, Nascimento VP. 2019. Antimicrobial Resistance and Molecular Characterization of Salmonella Enterica Serotypes Isolated from Poultry Sources in Brazil. Revista Brasileira de Ciência Avícola. 21(1): 001-008. ISSN 1516-635X. https://Doi.org/10.1590/1806-9061-2018-0827
CASTRO-VARGAS RE, Herrera-Sánchez MP, Rondón-Barragán IS. 2021. Plasmid-Mediated antibiotic resistance and class 1 integron in Salmonella Heidelberg isolated from poultry farms in Santander -Colombia. Brazilian Journal of Poultry Science. 3 (4): 001-008. P-ISSN: 1516-635X
O-ISSN: 1806-9061. https://doi.org/10.1590/1806-9061-2021-1460
CAMPIONI F, Moratto AM, Falcao JP. 2012. Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil. Food Microbiology. 32:254-264. ISSN 0740-0020. https://Doi.org/10.1016/j.fm.2012.06.008
CIAPAN. Guía para la asistencia técnica del Valle de Culiacán. Instituto Nacional de Investigaciones Agrícolas, Forestales y Pecuarias. Culiacán, Sinaloa, México. 2003. 92 pp. https://search.worldcat.org/es/title/guia-para-la-asistencia-tecnica-agropecuaria-para-el-area-de-influencia-del-campo-experimental-valle-de-culiacan/oclc/651323884
COELHO LR, Melo RT, Monteiro GP, Reischak D, Mendonça AO, Tavares AAS, Rossi D. 2021. Validation of alternative methods of detection of Salmonella Spp. in experimentally contaminated poultry environmental samples. Revista Brasileira de Ciência Avícola. 23(2):001-008. P-ISSN: 1516-635X. E-ISSN: 1806-9061. https://doi.org/10.1590/1806-9061-2020-1380
DAMAZIAK K, Riedel J, Gozdowski D, Niemiec J, Siennicka D, R´og D. 2017. Productive performance and egg quality of laying hens fed diets supplemented with garlic and onion extracts. Journal Applied of Poulty Research. 26:337–349. P-ISSN: 1537-0437. L-ISSN: 1056-6171. https://Doi:10.3382/japr/pfx001
DEVI GS, Prayag P, RemizRaja S, Ashifa KM, Devi KS. 2021. Inhibitory effect of garlic extract on multidrug resistant Salmonella isolated from broiler chicken meat. Journal of Current Research in Food Science. 2(2):27-33. P-ISSN:2709-9377. L-ISSN: 2709-9385. https://www.foodresearchjournal.com/article/41/2-2-3-958.pdf
DE KNEGT LV, Pires SM, Hald T. 2015. Attributing foodborne salmonellosis in humans to animal reservoirs in the European Union using a multi-country stochastic model. Epidemiol and Infection. 143:1175–86. P-ISSN: 09502688. E-ISSN: 14694409. https://Doi:10.1017/S0950268814001903
EKWENYE UN, Elegalam NN. 2005. Antibacterial Activity of Ginger (Zingiber officinale) Roscoe and Garlic (Allium sativum L.) Extracts on Escherichia coli and Salmonella typhi. International Journal of Molecular Medicine and Advance Sciences 2005; 1(4): 411-417. O-ISSN: 1994-4624. P-ISSN: 1813-176x. https://www.researchgate.net/publication/290488760_Antibacterial_activity_of_Ginger_Zingiber_officinale_Roscoe_and_Garlic_Allium_sativum_L_extracts_on_Escherichia_coli_and_Salmonella_typhi
FERREIRA SR, Toledo MC, Barbosa Da S TI, Jorge De FH, Saldanha de Sousa AD, Feres De MPG, Asis SL, Melo De QA. 2020. Salmonellosis occasioned by products of animal origin and its implications for public health: literature review. Brazilian Journal of Animal and Environmental Research. 3 (4); 3715-3746. https://DOI:10.34188/bjaerv3n4-075
FOOD AND DRUG ADMINISTRATION (FDA). 2007. Bacteriological Analytical Manual, capítulo 5: Salmonella. New Hampshire U.S.A. Disponible en https://www.fda.gov/food/laboratory-methods-food/bam-chapter-5-salmonella
RIVLIN RS. 2001. Historical perspective on the use of garlic. Journal of Nutrition. 131(3s):951-954. P-ISSN: 0022-3166. E-ISSN: 1541-6100. https://DOI:10.1093/jn/131.3.951S
GAMAZO C, Irache JM. Salmonella vaccines. 2007. Communicating current research and educational topics and trends in applied Microbiology. pp518-524. ISBN: 978-84-611-9422-3. http://www.formatex.org/microbio/pdf/. Acceso marzo 15 de 2017.
GARCÍA E. 2004. Modificaciones al sistema de clasificación climática de Köppen (para adaptarla a las condiciones climáticas de la República Mexicana). Instituto de Geografía. Universidad Nacional Autónoma de México.144 pp. ISBN: 970-32-1010-4. http://www.publicaciones.igg.unam.mx/index.php/ig/catalog/book/83
GARCÍA RR, Herrera FC. 2007. Evaluación de la inhibición del crecimiento de cinco cepas bacterianas patógenas por extractos acuosos de Allium sativum, Allium fistulosum y Allium cepa: estudio preliminar in vitro. Bistua, 5(2): 68-79. ISSN: 0120-4211. https://www.researchgate.net/publication/26544561_Evaluacion_de_la_inhibicion_del_crecimiento_de_cinco_cepas_bacterianas_patogenas_por_extractos_acuososde_Allium_sativum_Allium_fistulosum_y_Allium_cepaestudio_preliminar_in_vitro
GAST RK, Dittoe DK, Ricke SC. 2024. Salmonella in eggs and egg-laying chickens: pathways to effective control. Critical Reviews in Microbiology. 50 (1): 39–63. P-ISSN: 1040-841X. O-ISSN: 1549-7828. https://Doi.org/10.1080/1040841X.2022.2156772
GAST R, Guraya R, Jones D, Anderson K. 2015. Persistence of fecal shedding of Salmonella enteritidis by experimentally infected laying hens housed in conventional or enriched cages. Poultry Science. 94(7):1650–1656. P-ISSN: 1525-3171. L-ISSN: 0032-5791. https://Doi:10.3382/ps/pev113
GOMES DA SILVEIRA RD, Meneghetti C, Bonfim de Oliveira E, Amândio-Garcia PJA, Vasconcelos FF, Borges BD. 2021. Systematic review of the use of phytobiotics in broiler nutrition. Revista de Ciencias Agroveterinarias. 20(1):98-106. P-ISSN: 1676-9732. O-ISSN: 2238-1171. https://Doi:10.5965/223811712012021098
GONZÁLEZ-LÓPEZ I, Medrano-Félix JA, Castro-Del Campo N, López-Cuevas O, Ibarra-Rodríguez J, Martínez-Rodríguez C, Valdez-Torres JB, Cháidez, C. 2021. Metabolic plasticity of Salmonella enterica as adaptation strategy in river water. International Journal of Environmental Health Research. 1:1-14. P-ISSN: 0960-3123. O-ISSN: 1369-1619. https://DOI:10.1080/09603123.2021.1896682
HANNAN A, Rauf K, Ikram M, Naeem T, Raja M, Usman M, Tahir R, Saba M. 2012. Inhibitory effect of aqueous garlic (Allium Sativum) extract against clinical isolates of Salmonella typhi. African Journal of Microbiology Research. 6(21):4475–4480. P-ISSN: 1996-0808. L-ISSN: 1996-0808. https://Doi:10.5897/AJMR11.540
HASSAN MM, Esmaeili S, Bagheri AF, Mostafavi M, Zahraei ST. 2015. Detection of Salmonella spp in commercial eggs in Irán. Iran. Journal of Microbiology. 7(1):50-54. P-ISSN: 1735-8612. L-ISSN: 2345-4342. https://pmc.ncbi.nlm.nih.gov/articles/PMC4670468/
HOSSAIN J, Attia Y, Muhammad BF, Islam F, Sobur A, Islam A, Levy S, Rahman A, Nishiyama A, Islam S, Hassan J, Rahman T. 2021. Zoonotic Significance and Antimicrobial Resistance in Salmonella in Poultry in Bangladesh for the Period of 2011–2021. Zoonotic diseases. 1:3-24. P-
ISSN: 2813-0227. L-ISSN: 2717-2910. https://Doi:org/10.3390/zoonoticdis1010002
HOWARD ZR, O'Bryan CA, Crandall PG, Ricke SC. 2012. Salmonella Enteritidis in shell eggs: Current issues and prospects for control. Food Research International. 45: 755–764. P-ISSN: 0963-9969. L-ISSN: 1873-7145. https://doi:10.1016/j.foodres.2011.04.030
HULAJ B, Abeli PC, Goga I, Taylor N, Hess C, Hess MI. 2016. Survey of the prevalence of Salmonella species on laying hen farms in Kosovo. Poultry Science. 95: 2030–2037. P-ISSN: 2957-6334. L-ISSN: 1525-3171. https://Doi.org/10.3382/ps/pew149
IM MC, Jeong J, Yong-Kuk K, Ok-Mi J, Min-Su K, Young JL. 2015. Prevalence and characteristics of Salmonella spp. Isolated from commercial layer farms in Korea. Poulty Science. 94:1691-1698. P-ISSN: 2957-6334. L-ISSN: 1525-3171. https://DOI:10.3382/ps/pev137
KAGAMBÈGA A, Thibodeau A, Trinetta V, Soro DK, Sama FN, Bako É, Bouda CS, N’Diaye AW, Fravalo F, Barro N. 2018. Salmonella spp. and Campylobacter spp. in poultry feces and carcasses in Ouagadougou, Burkina Faso. Food Science & Nutrition. 6:1601-1606. ISSN: 2048-7177. https://Doi:10.1002/fsn3.725
MAURISCHAT S, Szabo I, Baumman B, Malorny B. 2015. Rapid real-time PCR methods to distinguish Salmonella Enteritidis wildtype field isolates from vaccine strains Salmovac SE/Gallivac SE and AviPro SALMONELLA VAC E. Journal of Microbiological Methods. 112(5):92-98. P-ISSN: 0167-7012. L-ISSN: 1872-8359. https://Doi:10.1016/j.mimet.2015.03.015
MAGRYŚ A, Olender A, Tchórzewska D. 2021. Antibacterial properties of Allium sativum L. against the most emerging multidrug‑resistant bacteria and its synergy with antibiotics. Archives of Microbiology. 203: 2257–2268. P-ISSN: 0302-8933. L-ISSN: 1432-072X. https://Doi.org/10.1007/s00203-021-02248-z.
MOHAMMADIGHEISAR M, Kim IN HO. 2017. Phytobiotics in poultry and swine nutrition a review. Italian Journal of Animal Science. 17(1):92-99. ISSN: 1594-4077. L-ISSN: 1828-051X. https://Doi:10.1080/1828051X.2017.1350120
NORMA OFICIAL MEXICANA (NOM-210-SSA1-2014). 2015. Bienes y servicios. “Métodos de prueba microbiológicos. Determinación de microorganismos indicadores. Determinación de microorganismos patógenos” Diario Oficial de la Federación. Disponible en: https://dof.gob.mx/nota_detalle.php?codigo=5398468&fecha=26/06/2015#gsc.tab=0
NEVELIN DP, Dicks MT. 2021. Probiotics: an antibiotic replacement strategy for healthy broilers and productive rearing. Probiotics and Antimicrobial Proteins. 13:1-11. P-ISSN: 1867-1306. L-ISSN: 1867-1314. https://doi.org/10.1007/s12602-020-09640-z
PETROPOULOS AS, Fernández L, Barros A, Ciric M, Sokovic-Ferreira IS. 2018. Antimicrobial and antioxidant properties of various greek garlic genotypes. Food chemistry. 245: 7-12. P-ISSN: 0308-8146. L-ISSN: 1873-7072. https://Doi.org/10.1016/j.foodchem.2017.10.078
RIVERA CLG, Ortegón CLH, Estrada CG, Granja YTS, Núñez JM. 2013. Aislamiento, identificación y patrón de sensibilidad antimicrobiana de Salmonella spp en primates en cautiverio. Revista Colombiana de Ciencia Animal. 5(1):131-144. P-ISSN: 2027-1840. L-ISSN: 2462-7623. https://dialnet.unirioja.es/servlet/articulo?codigo=4694099
RIVLIN RS. 2001. Historical perspective on the use of garlic. The journal of nutrition. 131(3s):951-954. P-ISSN: 0022-3166. L-ISSN: 1541-6100. https://Doi:10.1093/jn/131.3.951S
RODRÍGUEZ HR. 2015. Prevalencia y caracterización molecular de Salmonella spp. en granjas avÍcolas de postura commercial en el departamento de Tolima. Tesis de Maestría en Ciencias Pecuarias, Universidad de Tolima, Ibagué, Colombia
ROMO-RUBIO JA, Silva-Hidalgo G, López MHS, Simental OL, Beltrán FS, Garfio RA. 2025. Prevalence, serovars, and antibiotic resistance profiles of Salmonella spp. isolated from pigs in Sinaloa, Mexico. Brazilian Journal of Animals and Environmental Research. 8 (4); 1-17. https://Doi.org/10.34188/bjaerv8n4-027
SÁENZ L, Guzmán M, Vidal S, Caruffo M, Siel D, Zayas C, Paredes R, Valenzuela C, Hidalgo H, Pérez O, Lapierre L. 2022. Efficacy of multivalent, cochleate-based vaccine against Salmonella Infantis, S. Enteritidis and S. Typhimurium in laying hens. Vaccines. 10(226):1-13. P-ISSN: 0899-4056. https://doi.org/10.3390/vaccines10020226
SALEM VW, Dina M, Shibat El-hamed W, Sayed R. 2017. Alterations in virulence and antibiotic resistant genes of multidrug-resistant Salmonella serovars isolated from poultry: The bactericidal efficacy of Allium sativum. Microbial Pathogenesis. 108:91-100. P-ISSN: 0882-4010. L-ISSN:1096-1208. https://doi.org/10.1016/j.micpath.2017.05.008
SAS INSTITUTE INC. (2002). The SAS System for Windows 9.0. Cary, N.C. USA. https://www.sas.com/es_mx/software/stat.html
SAMBROOK J, Fritsch E, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2d Edition, Cold spring harbor laboratory press. ISBN: 978-1-936113-42-2. https://doi.org/10.1016/0307-4412(83)90068-7
SARKER BR, Ghosh S, Chowdhury S, Dutta A, Chamdra DL, Saker BK, Sultana T, Hossain MM. 2021. Prevalence and antimicrobial susceptibility profiles of non- typhoidal Salmonella isolated from chickens in Rajshahi, Bangladesh. Veterinary Medicine and Science. 7(1):820–830. E-ISSN:2053-1095. https://Doi:10.1002/vms3.440
SORIA MC, Soria MA, Bueno DJ, Godano EI, Gómez SC, ViaButron IA, Padin BM, Rogé AD. 2017. Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods. Poultry Science. 00: 1–11. L-ISSN: 1525-3171. https://Doi.org/10.3382/ps/pex053
STEEL RGD, Torrie JH. 1998. Bioestadística principios y procedimientos. Segunda Edición. Editorial Mc Graw - Hill. México. https://biblioteca.usco.edu.co/cgi-bin/koha/opac-detail.pl?biblionumber=25861
THUNG TI, Mahyudin NA, Basri DF, Wan Mohamed Radzi CW, Nakaguchi Y, Nishibushi M, Radu S. 2016. Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia. Poultry Science. 95:1888-1893. L-ISSN: 1525-3171. https://doi:10.3382/ps/pew144
TOSUN SY, Alakavuk DU, Ulusoy S, Erkan N. 2017. Effects of essential oils on the survival of Salmonella Enteritidis and Listeria monocytogenes on fresh atlantic salmon (Salmo salar) during storage at 2 ± °C. Journal of Food Safety. 38(1):1-6. P-ISSN: 0149-6085. L-ISSN: 1745-4565. https://Doi.org/10.1111/jfs.12408
VAN IMMERSEEL F, Methner U, Rychlik I, Nagy B, Velge P, Martin G, Foster N, Ducatelle R, Barrow P. 2005. Vaccination and early protection against non-host specific Salmonella serotypes in poultry: exploitation of innate immunity and microbial activity. Epidemiology and Infection. 133(6):959–978. P-ISSN: 0950-2688. L-ISSN: 1469-4409. https://doi:10.1017/S0950268805004711
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