Controle químico, biológico e a biotecnologia no manejo inicial da Spodoptera frugiperda em milho no Sudoeste Goiano

Autores

  • Tiago Assis Zilio
  • Marcelo Coutinho Picanço

DOI:

https://doi.org/10.34188/bjaerv8n4-096

Palavras-chave:

Spodoptera frugiperda, inimigos naturais, piretróides, organofosforados, resistência, plantas Bt

Resumo

As lagartas militares como a Spodoptera frugiperda e similares, causam elevadas perdas econômicas na cultura do milho no Sudoeste Goiano. Isso se deve ao aumento da resistência a inseticidas químicos e a baixa seletividade dos inimigos naturais aos mesmos, uso inadequado e a não adoção de biotecnologia em híbridos que matam as lagartas e a dificuldade do sistema produtivo de aliar os diferentes métodos de controle. O uso de modos de ação e mecanismos de ação dos grupos químicos dos organofosforados e piretróides em seus princípios ativos mais utilizados aliado ao controle com uso de híbridos de milho transgênico, o biocontrole e controle natural com Dorus luteipes, Metarhizium rileyi, Beauveria bassiana, Nomuraea rileyi, B. Thuringiensis e outros inimigos naturais são algumas ações interessantes para manejo da praga alvo. Aplicação de inseticidas mais o uso de plantas Bt trazem resultados que superam os demais manejos testados comparados e sua má utilização acelera as mutações e resistência da Spodoptera frugiperda aos métodos de controle químico e genético, além de diminuir a biodiversidade de inimigos naturais. A combinação correta do químico estressor com o inimigo natural e a seleção de espécies com maior potencial de utilização, são importantes para a eficácia em utilização e desenvolvimento de produtos. Para um bom controle inicial da Spodoptera frugiperda na cultura do milho é importante usar a correta área de refúgio de lepidópteras não resistentes ao Bt, uso de sementes tratadas com inseticida sistêmico, amostrar as lagartas vivas, as mortas por inimigos naturais e os diferentes inimigos naturais antes e após a primeira aplicação de inseticida foliar, avaliar os danos da praga nos diferentes híbridos de plantas, observar o Nível de Não Ação (NNA) e registrar o calendário da primeira aplicação em cada talhão quando a densidade da praga exigir o controle químico para que não cause danos econômicos consideráveis. Frente ao exposto podemos definir quais híbridos, grupos químicos e princípios ativos são mais eficazes no controle da Spodoptera frugiperda, quais tratamentos de semente e primeiras aplicações foliares afetam menos os inimigos naturais por talhão e quais os talhões em que é possível retardar a aplicação, devido as condições genéticas e biológicas do sistema de produção.

     

Biografia do Autor

Tiago Assis Zilio

Marcelo Coutinho Picanço

Referências

Abdalla, H., et al., 2008. Insecticide susceptibility and vector status of natural populations of Anopheles arabiensis from Sudan. Trans. R. Soc. Trop. Med. Hyg. 102, 263–271.

Altre J A, Vandenberg J D. 2001. Comparison of blastospores of two Paecilomyces fumosoroseus isolates: in vitro traits and virulence when injected into fall armyworm, Spodoptera frugiperda. Journal of Invertebrate Pathology, 78, 170–175.

Barrera G P, Belaich M N, Patarroyo M A, Villamizar L F,Ghiringhelli P D. 2015. Evidence of recent interspecieshorizontal gene transfer regarding nucleopolyhedrovirus infection of Spodoptera frugiperda. BMC Genomics, 16,1008.

Berretta M F, Rios M L, de Cap A S. 1998. Characterization of a nuclear polyhedrosis virus of Spodoptera frugiperda fromArgentina. Journal of Invertebrate Pathology, 71, 280–282.

Beserra E B, Dias C T, Parra J R. 2005. Behavior of Trichogramma atopovirilia Oatman & Platner and T. pretiosum Riley (Hymenoptera: Trichogrammatidae) on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) egg masses. Brazilian Journal of Biology, 65,9–17.

BORTOLOTTO, O.C. et al. Larval development of Spodoptera eridania (Cramer) fed on leaves of Bt maize expressing Cry1F and Cry1F + Cry1A.105

+ Cry2Ab2 proteins and its non-Btisoline. Revista Brasileira de Entomologia, v.59, n.1, p.7-11, 2015.

BORGES, A. Changes in hematological and serum biochemical values in Jundiá Rhamdia quelen due to sub-lethal toxicity of cipermethrin. Chemosphere, v. 69, p. 920-926, 2007.

B. M. O. Agriculture, 2013. Agrofit: Sistema de Agrotóxicos Fitossanitários. Blanco, C.A., et al., 2014. Maize pests in Mexico and challenges for the adoption of integrated pest management programs. J. Integr. Pest Manag. 5, 1–9.

BRADBERRY, S. M.; CAGE, S. A.; PROUDFOOT, A. T.; VALE, J. A. Poisoningvdue to Pyrethroids. Toxicological Reviews, v. 24, n. 2, p. 93-106, 2005.

BRAGA, I.A, VALLE, D. Aedes aegypti: Histórico do controle no Brasil.vEpidemiol. Serv. Saúde; 16(2) : 113 – 118, 2007a.

BRAGA, I.A., VALLE, D. Aedes aegypti: inseticidas, mecanismos de ação e resistência. Epidemiol. Serv. Saúde, 16(4):279-293, 2007b

Brito, L.P., Carrara, L., de Freitas, R.M., Lima, J.B.P., Martins, A.J., 2018. Levels of resistance to Pyrethroid among distinct kdr alleles in Aedes aegypti laboratory lines and frequency of kdr alleles in 27 natural populations from Rio de Janeiro, Brazil. Biomed. Res. Int. 2410819 (2018).

Carvalho, R.A., Omoto, C., Field, L.M., Williamson, M.S., Bass, C., 2013. Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda. PLoS One 8, e62268.

Carvalho, R.A., Omoto, C., Field, L.M., Williamson, M.S., Bass, C., 2013. Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda. PloS One 8, e62268. https://doi.org/ 10.1371/journal.pone.0062268.

Carneiro A A, Gomes E A, Guimaraes C T, Fernandes F T, Carneiro N P, Cruz I. 2008. Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. Pesquisa Agropecuaria Brasileira, 43, 513–520.

Castillejos V, Trujillo J, Ortega L D, Santizo J A, CisnerosJ, Penagos D I, Valle J, Williams T. 2002. Granular phagostimulant nucleopolyhedrovirus formulations forcontrol of Spodoptera frugiperda in maize. Biological Control, 24, 300–310.

CRUZ, I. Manejo de pragas na cultura do milho. In. Seminário sobre a cultura do milho safrinha, 5, Barretos-SP, 1999. crusos . Campinas: Instituto Agronômico, 1999. p.27-56.

CRUZ, I. Manual de identificação de pragas do milho e de seus principais agentes de controle biológico. Brasília: EMBRAPA, 2008. 192p.

CRUZ, I., FIGUEIREDO, M.L., MATOSO, M.J. Controle Biológico de Spodoptera frugiperda Utilizando o Parasitoide de Ovos Trichogramma. Sete Lagoas: EMBRAPA-CNPMS, 1999. 40p. Circulara Técnica, 30.

DAVIES, T.G.E., FIELD, L.M., USHERWOOD, P.N.R., WILLIAMSON, M.S.

DDT, Pyrethrins, Pyrethroids and Insect Sodium Channels. Life, 59(3): 151- 162, 2007.

De Bortoli C P, Jurat-Fuentes J L. 2019. Mechanisms of resistance to commercially relevant entomopathogenic bacteria. Current Opinion in Insect Science, 33, 56–62.

DONG, K. et al. Molecular biology of insect sodium channels and pyrethroid

resistance. Insect Biochemical and Molecular Biology, v. 50, p. 1-17, 2014.

DONG, K., 2007. Insect sodium channels and insecticide resistance. Invertebr. Neurosci.:IN 7, 17–30.ffrench-Constant, R.H., Pittendrigh, B., Vaughan, A., Anthony, N., 1998. Why are there sofew resistance-associated mutations in insecticide target genes? Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci. 353, 1685–1693.

EPPO, 2019. Spodoptera frugiperda continues to spread in Asia. In: EPPO Reporting Service, (No. 2019/053). EPPO, Paris, France. https://gd.eppo.int/reporting/ article-6483.

Farias, J.R., Andow, D.A., Horikoshi, R.J., Sorgatto, R.J., Fresia, P., dos Santos, A.C., Omoto, C., 2014. Field-evolved resistance to Cry1F maize by Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil. Crop Protect. 64, 150e158. https:// doi.org/10.1016/j.cropro.2014.06.019.

Figueiredo, M.L.C., Penteado-Dias, A.M., Cruz, I., 2005. Danos provocados por Spodoptera frugiperda na produção de materia seca e nos rendimentos de grãos, na cultura do milho. Embrapa 130, 1e6.

GARCEZ, M. Brasil é o maior consumidor de agrotóxicos do mundo; entenda o impacto. 2022. Disponível em <https://doutorjairo.uol.com.br>. Acesso em 09 de Junho de 2023.

Fronza, E., Specht, A., Heinzen, H., de Barros, N.M., 2017. Metarhizium (Nomuraea) rileyi as biological control agent. Biocontrol Sci. Technol. 27, 1243e1264. https:/doi.org/10.1080/09583157.2017.1391175

Foil, L.D., Guerrero, F., Alison, M.W., Kimball, M.D., 2005. Association of the kdr andsuperkdr sodium channel mutations with resistance to pyrethroids in Louisiana populations of the horn fly, Haematobia irritans irritans (L.). Vet. Parasitol. 129,149–158.

FORNASIERI FILHO, Domingos. Manual da Cultura do Milho. Funep, Jaboticabal SP, 2007. 576p.

Foster, S.P., et al., 2014. A mutation (L1014F) in the voltage-gated sodium channel of thegrain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides. Pest Manag. Sci. 70, 1249–1253.

Furlan, L., Canzi, S., di Bernardo, A., Edwards, C.R., 2006. The ineffectiveness of insecticide seed coatings and planting-time soil insecticides as Diabrotica virgifera virgifera LeConte population suppressors. J. Appl. Entomol. 130, 485–490.

GALLO, D.; NAKANO, O.; SILVEIRA NETO, S.; CARVALHO, R.P.L.; BAPTISTA,

G.C. de; BERTI FILHO, E.; PARRA, J.R.P.; ZUCCHI, R.A.; ALVES, S.B.; VENDRAMIM, J.D.; MARCHINI, L.C.; LOPES, J.R.S.; OMOTO, C. Entomologia

agrícola. Piracicaba, Fealq, 2002, 920p.

Geiger, F., et al., 2010. Persistent negative effects of pesticides on biodiversity and biological control potential on European farmland. Basic Appl. Ecol. 11, 97–105.

Gould, F., Kennedy, G.G., Johnson, M.T., 1991. Effects of natural enemies on the rate of herbivore adaptation to residual host plants. Entomol. Exp. Appl. 58, 1e14

Goergen, G., Kumar, P.L., Sankung, S.B., Togola, A., Tamo, M., 2016. First report of outbreaks of the fall armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa. PloS One 11, 1e9. https://doi.org/10.1371/journal.pone.0165632.

Grijalba E P, Espinel C, Cuartas P E, Chaparro M L, Villamizar L F. 2018. Metarhizium rileyi biopesticide to control Spodoptera frugiperda: stability and insecticidal activity under glasshouse conditions. Fungal Biology, 122, 1069–1076.

GULLAN, P. J.; CRANSTON , P. S. Os insetos; um resumo de entomologia. 3 ed., Roca, São Paulo, 2007. p.440.

Kasai, S., Sun, H., Scott, J.G., 2017. Diversity of knockdown resistance alleles in a singlehouse fly population facilitates adaptation to pyrethroid insecticides. Insect Mol. Biol.26, 13–24.

Kalamarakis, A.E., Markellou, E., 2007. Efficacy evaluation of plant protection products at EU level: data requirements and evaluation principles. J. Pestic. Sci. 32, 1–9.

Lamberth, C., 2018. Agrochemical lead optimization by scaffold hopping. Pest Manag. Sci. 74, 282–292.

Li Y P, Zhang S, Wang X J, Xie X P, Liang P, Zhang L, Gu S H, Gao X W. 2019. Current status of insecticide resistance in Spodoptera frugiperda and strategies for its chemical control. Plant Protection, 45, 14–19. (in Chinese)

LOURENÇÃO, A. L. F.; FERNANDES, M. G. Avaliação do milho Bt Cry1Ab e Cry 1F no controle de Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) em condições de campo. Científica, 41(2):164-188, 2013

Ministério da Agricultura Pecuária e Abastecimento. Disponível em http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons. acesso em 22 de outubro de 2009.

Matambo, T.S., et al., 2007. Insecticide resistance in the malarial mosquito Anophelesarabiensis and association with the kdr mutation. Med. Vet. Entomol. 21, 97–102.

Martins, A.J., et al., 2012. Effect of insecticide resistance on development, longevity and reproduction of field or laboratory selected Aedes aegypti populations. PLoS One 7,e31889.

Molina-Ochoa J, Carpenter J E, Heinrichs E A, Foster J E.2003. Parasitoids and parasites of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas and Caribbean Basin: An inventory. Florida Entomologist, 86, 254–289.

Nicholson, R.A., Miller, T.A., 1985. Multifactorial resis- tance to transpermethrin in fieldcollected strains of the tobacco budworm Heliothis virescens F. Pestic. Sci. 16, 561.

Negre, V., et al., 2006. SPODOBASE: an EST database for the lepidopteran crop pestSpodoptera. BMC Bioinformatics 7, 322.

Omoto, C., Bernardi, O., Salmeron, E., Sorgatto, R.J., Dourado, P.M., Crivellari, A., Carvalho, R.A., Willse, A., Martinelli, S., Head, G.P., 2016. Field-evolved resistance to Cry1Ab maize by Spodoptera frugiperda in Brazil. Pest Manag. Sci. 72, 1727e1736. https://doi.org/10.1002/ps.4201.

Ottea, J.A., Ibrahim, S.A., Younis, A.M., Leonard, B.R., McCaffery, A.R., 1995. Biochemical and physiological mechanisms of pyrethroid resistance in Heliothisvirescens (F,). Pestic. Biochem. Physiol. 51.

Ottea, J.A., Holloway, J.W., 1998. Target-site resistance to Pyrethroids in Heliothisvirescens (F.) and Helicoverpa zea (Boddie). Pestic. Biochem. Physiol. 61, 155–167.

Pasini A, Parra J R, Lopes J M. 2007. Artificial diet for rearing Doru luteipes (Scudder) (Dermaptera: Forficulidae), a predator of the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). Neotropical Entomology, 36, 308–311.

Pesticide Resistance. Disponível em: https://www.pesticideresistance.org/. Acesso em 22 de outubro de 2009.

Pitre H N. 1986. Chemical control of the fall armyworm (Lepidoptera: Noctuidae): An update. Florida Entomologist, 69, 570–578.

Prasanna B, Huesing J, Eddy R, Peschke V. 2018. Fall Armyworm in Africa: A Guide for Integrated Pest Management. CAB International, Wallingford. pp. 1– 109.

Rios-Diez, J.D., Saldamando-Benjumea, C.I., 2011. Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) strains from Central Colombia to two insecticides,methomyl and lambda-cyhalothrin: a study of the genetic basis of resistance. J. Econ. Entomol. 104, 1698–1705.

Russo, M. L., Jaber, L. R., Scorsetti, A. C., Vianna, F., Cabello, M. N., & Pelizza, S. A. (2020). Effect of entomopathogenic fungi introduced as corn endophytes on the development, reproduction, and food preference of the invasive fall armyworm Spodoptera frugiperda. Journal of Pest Science, 1-12.

Sanchez-Bayo, F., Goka, K., 2014. Pesticide residues and bees – a risk assessment. PloS One 9, e94482.

Sparks, T.C., Nauen, R., 2015. IRAC: mode of action classification and insecticide resistance management. Pestic. Biochem. Physiol. 121, 122–128.

Singh G, Sachdev B, Sharma N, Seth R, Bhatnagar R K. 2010 .Interaction of Bacillus thuringiensis vegetative insecticidal protein with ribosomal S2 protein triggers larvicidal activity in Spodoptera frugiperda. Applied and Environmental Microbiology, 76, 7202–7209.

SIEBERT, M.W. Evaluation of corn hybrids expressing Cry1F, Cry1A.105, Cry2Ab2, Cry34Ab1/Cry35Ab1, and Cry3Bb1 against southern United States insect pests. Journal of Economic Entomology, v.105, n.5, p.1825-1834, 2012. Available from: ˂Available from: ˂http://www.ncbi.nlm.nih.gov/pubmed/23156183˃. Accessed: Apr. 12, 2015. doi: 10.1603/EC12155

Simon O, Palma L, Williams T, Lopez-Ferber M, Caballero P. 2012. Analysis of a naturally-occurring deletion mutant of Spodoptera frugiperda multiple nucleopolyhedrovirus reveals sf58 as a new per os infectivity factor of lepidopteraninfecting baculoviruses. Journal of Invertebrate Pathology, 109, 117–126

Sparks, T.C., Lorsbach, B.A., 2016. Perspectives on the agrochemical industry and agrochemical discovery. Pest Manag. Sci. 73, 672–677.

Storer N P, Babcock J M, Schlenz M, Meade T, Thompson G D, Bing J W, Huckaba R M. 2010. Discovery and characterization of field resistance to Bt maize: Spodoptera frugiperda (Lepidoptera: Noctuidae) in Puerto Rico. Journal of Economic Entomology, 103, 1031–1038.

Sueldo M R, Bruzzone O A, Virla E G. 2010. Characterization of the earwig, Doru lineare, as a predator of larvae of the fall armyworm, Spodoptera frugiperda: A functional response study. Journal of Insect Science, 10, 38.

Tendeng E, Labou B, Diatte M, Djiba S, Diarra K. 2019. The fall armyworm Spodoptera frugiperda (JE Smith), a new pest of maize in Africa: Biology and first native natural enemies detected. International Journal of Biological and Chemical Sciences, 13, 1011–1026.

Tinsley, N.A., Mitchell, P.D., Wright, R.J., Meinke, L.J., Estes, R.E., Gray, M.E., 2016. Estimation of efficacy functions for products used to manage corn rootworm larval injury. J. Appl. Entomol. 140, 414–425.

Vachon V, Laprade R, Schwartz J L. 2012. Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: A critical review. Journal of Invertebrate Pathology, 111, 1–12.

Wang J, Wang H, Liu S, Liu L, Tay W T, Walsh T K, Yang Y, Wu Y D. 2017. CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins. Insect Biochemistry and Molecular Biology, 87, 147–153

Wilson, L.J., Whitehouse, M.E.A., Herron, G.A., 2018. The management of insect pests in Australian cotton: an evolving story. Annu. Rev. Entomol. 63, 215–237

Wild S. 2017. African countries mobilize to battle invasive caterpillar. Nature, 543, 13–14.

Wilkinson, I.J., McCaffery, A.R., 1991. Titres of cis-cypermethrin in the CNS of resistant and susceptible strains of Heliothis virescens: pharmacokinetic modification of target site exposure. Pestic. Sci. 34.

Williamson, M.S., Denholm, I., Bell, C.A., Devonshire, A.L., 1993. Knockdown resistance (kdr) to DDT and pyrethroid insecticides maps to a sodium channel gene locus in thehousefly (Musca domestica). Mol. Gen. Genet. 240, 17–22.

Soderlun, D.M., Lee, S.H., 2001. Point mutations in homology domain II modify the sensitivity of rat Nav1.8 sodium channels to the pyrethroid insecticide cismethrin. Neurotoxicology 22, 755–765.

SPARKS, T. C.; NAUEN, R. IRAC: Mode of action classification and insecticide resistance management. Pesticide Biochemistry and Physiology, v. 121, p. 122-128, 2015.

Xiao Y T, Dai Q, Hu R Q, Sabino P, Yang Y B, Liang G M, Mario S, Alejandra B, Liu K Y, Wu K M. 2017. A single point mutation resulting in cadherin mislocalization underpins resistance against Bacillus thuringiensis toxin in cotton bollworm. The Journal of Biological Chemistry, 292, 2933–2943.

Yu, S.J., Nguyen, S.N., Abo-Elghar, E.G., 2003. Biochemical characteristics of insecticideresistance in the fall armyworm, Spodoptera frugiperda. Pestic. Biochem. Physiol. 77,1–11.

Yu S. 1991. Insecticide resistance in the fall armyworm, Spodoptera frugiperda (JE Smith). Pesticide Biochemistry and Physiology, 39, 84–91.

Yu S J, McCord Jr E. 2007. Lack of cross-resistance to indoxacarb in insecticide-resistant Spodoptera frugiperda (Lepidoptera: Noctuidae) and Plutella xylostella (Lepidoptera: Yponomeutidae). Pest Management Science, 63, 63–67.

Yu S J, Nguyen S N, Abo-Elghar G E. 2003. Biochemical characteristics of insecticide resistance in the fall armyworm, Spodoptera frugiperda (J.E. Smith). Pesticide Biochemistry

Zhang L, Liu B, Zheng W G, Liu C H, Zhang D D, Zhao S Y, Li Z Y, Xu P J, Wilson K, Withers A, Jones C M, Smith J A, Chipabika G, Kachigamba D L, Nam K, d’Alençon E, Liu B, Liang X Y, Jin M H, Wu C, et al. 2020. Genetic structure and

insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources, 20, 682–1696

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2025-11-17

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Zilio, T. A., & Picanço, M. C. (2025). Controle químico, biológico e a biotecnologia no manejo inicial da Spodoptera frugiperda em milho no Sudoeste Goiano. Brazilian Journal of Animal and Environmental Research, 8(4), e83755. https://doi.org/10.34188/bjaerv8n4-096

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