Análisis de los efectos genotóxicos del malatión en Rhamdia quelen mediante el test de micronúcleos
DOI:
https://doi.org/10.34188/bjaerv8n1-135Palavras-chave:
malatión, micronúcleos, alteraciones morfológicas nucleares, Rhamdia quelenResumo
El uso de pesticidas es ampliamente utilizado para el control de plagas entre ellos los organofosforados. Su uso podría ocasionar un riesgo potencial para el ambiente. El malatión es un insecticida organofosforado con baja toxicidad en mamíferos y notable selectividad para los insectos, no obstante, la contaminación del medio acuático por sus residuos podría afectar la calidad del agua alterando el hábitat. Por lo tanto, el objetivo de este trabajo consistió en evaluar los efectos genotóxicos del malatión (Glex M 100) en células sanguíneas (eritrocitos) de ejemplares juveniles Rhamdia quelen. Un total de 10 peces se expusieron a las concentraciones de 4 mg L-1 de malatión (Glex M 100) durante 39 días, después de este período, se extrajo sangre por punción de la vena caudal para la confección de los frotis. El análisis de los mismos reveló la ocurrencia de células con micronúcleos, vacuolas nucleares, lobulaciones nucleares (blebbed nuclei), muescas nucleares (notched nuclei). Estas anormalidades se presentaron con mayor frecuencia en el grupo expuesto al malatión con respecto al control. El estudio estadístico mostró diferencias significativas entre los grupos de control y los tratados, lo cual indica la existencia de un daño en el material genético causado por la exposición de los peces al pesticida. Por lo cual, los resultados obtenidos en R. quelen nos permiten inferir sobre la capacidad del malatión de inducir daño en el material genético, con la eventual posibilidad de desencadenar efectos nocivos en el medio ambiente.
Referências
Alberts B, et. al. Essential Cell Biology: An Introduction to the Molecular Biology of the Cell Garland Science. New York, USA: Garland Science, 1997.
Al-Sabti K, Metcalfe CD. Fish micronuclei for assessing genotoxicity in water. Mutation Research/Genetic Toxicology, Netherlands, 343: 121-135, junio 1995
Ayllon F, Garcia-Vazquez E. Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fish micronucleus test. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Netherlands, 467(2), 177-186, mayo 2000
Badii MH y Flores JL. Plaguicidas que afectan a la salud humana y la sustentabilidad. CULCyT: Cultura Científica y Tecnológica. Ciudad de Juárez (México). 4(19), 21-34, marzo-abril 2007.
Barbieri E, Ferreira LAA. Effects of the organophosphate pesticide Folidol 600® on the freshwater fish, Nile Tilapia (Oreochromis niloticus). Pesticide Biochemistry and Physiology, United States, 99(3), 209-214, marzo 2011.
Binelli A, Provini A. Risk for human health of some POPs due to fish from Lake Iseo. Ecotoxicology and Environmental safety. United States, 58(1), 139-145, mayo 2004.
Blasiak J, Jaloszynski P, Trzeciak A, Szyfter K. In vitro studies on the genotoxicity of the organophosphorus insecticide malathion and its two analogues. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. Netherlands. 445(2), 275-283, Septiembre de 1999.
Brzuzan P, Jurczyk L, Wozny M. Molecular geometry, CYP1A gene induction and clastogenic activity of cyclopenta [c] phenanthrene in rainbow trout. Polycyclic Aromatic Compounds, United Kingdom, 26(5), 345-365, diciembre 2006
Carrasco KR, Tilbury KL, Myers MS. Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects. Canadian Journal of Fisheries and Aquatic Sciences, Canadá, 47(11), 2123-2136, noviembre 1990
Çavao T, Ergene-Gozukara S. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquatic toxicology, Netherlands, 74(3), 264-271, septiembre 2005
Chen XY, Shao JZ, Xiang LX, Liu XM. Involvement of apoptosis in malathion-induced cytotoxicity in grass carp (Ctenopharyngodon idellus) cell line. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, United States, 142(1-2), 36-45, enero-febrero 2006
Da Silva LACL, Bonan RF., Pinto MBR, Kowalski LP, da Cruz Perez DE.The pesticides use and the risk for head and neck cancer: A review of case-control studies. Medicina Oral, Patología Oral y Cirugía Bucal, Spain, 26(1), e56, julio 2020
de Mello NP, de Carvalho ACC, Santarossa MA da S, Machado AA, Machado Neto JG. Toxicidade aguda e risco ambiental do malathion aplicado em nebulização contra Aedes aegytpi para organismos aquáticos / Acute toxicity and environmental risk of malathion applied in nebulization against Aedes aegytpi for aquatic organisms. Brazilian Journal of Animal and Environmental Research, Brasil, 4(1), 571–578, febrero de 2021.
Dulout FN, Oliveira OA, von Guradze H, Pastori MC. Cytogenetic effect of malathion assessed by the micrnucleus test. Mutation Research Letters, Netherlands, 105(6), 413-416, diciembre de 1982.
Espina NG, Wesis P. DNA repair in fish from polluted estuaries. Marine Environmental Research, Netherlands, 39(1-4), 309-312, 1995.
Farkas A, Salanki J, Specziar A. Relation between growth and the heavy metal concentration in organs of bream Abramis brama L. populating lake Balaton. Archives of environmental contamination and toxicology, United States, 43, 236-243, agosto de 2002.
Fenech M, Crott JW. Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes-evidence for breakage-fusion-bridge cycles in the cytokinesis-block micronucleus assay. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Netherlands, 504(1-2), 131-136, julio 2002.
Ferrero AA, Gutiérrez MM, Cervellini PM. Evaluación en laboratorio de la toxicidad aguda de los insecticidas malatión y deltametrina en Chasmagnathus granulata Dana (Crustacea, Brachyura, Grapsidae). Investigaciones marinas, Valparaíso (Chile), 29(1), 107-111, marzo 2001.
Galloway SM, Armstrong MA, Reuben C, Colman S, Brown B, Cannon I, Ahmed M, Duk S, Rimpo J, Margolin BH, Resnick MA, Anderson B, Zeiger E. Chromosome aberrations and sister chromatid exchanges in chinese hamster ovary cells: Evaluations of 108 chemicals. Environmental and molecular mutagenesis, United States, 10(S10), 1-35, abril 1987.
Garaj-Vrhovac V, Zeljezic D. Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay: Pesticide genotoxicity revealed by comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Netherlands, 469(2), 279-285, septiembre 2000.
Giri A, Giri S and Sharma, GD. Malathion and fenvalerate induce micronuclei in mouse bone marrow cells. Environmental and molecular mutagenesis, United States, 52(8), 607-613, abril 2011.
Goksøyr, A., Andersson, T., Buhler, D. R., Stegeman, J. J., Williams, D. E., & Förlin, L. Immunochemical cross-reactivity of β-naphthoflavone-inducible cytochrome P450 (P450IA) in liver microsomes from different fish species and rat. Fish Physiology and Biochemistry, Netherlands, 9, 1-13, marzo 1991.
Heddle JA. A rapid in vivo test for chromosomal damage. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Netherlands, 18(2), 187-190, mayo 1973.
Isani G, Andreani G, Cocchioni F, Fedeli D, Carpené, E, Falcioni G. Cadmium accumulation and biochemical responses in Sparus aurata following sub-lethal Cd exposure. Ecotoxicology and environmental safety, United States, 72(1), 224-230, enero 2009.
Kumar R, Nagpure NS, Kushwaha B, Srivastava SK, Lakra WS. Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and comet assay. Archives of environmental contamination and toxicology, United States, 58, 123-130, enero 2010.
Leiss JK, Savitz DA. Home pesticide use and childhood cancer: A case-control study. American Journal of public health, United States 85(2), 249-252, febrero 1995.
Lindberg HK, Wang X, Jarventaus H, Falck GC, Norpa H, Fenech M. Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Netherlands, 617(1-2), 33-45, abril 2007.
Matheus Lobo T, Bolaños A. Micronúcleos: biomarcador de genotoxicidad en expuestos a plaguicidas. Salus, Venezuela, 18(2), 18-26, agosto 2014.
Moore PD, Yedjou CG, Tchounwou PB. Malathion-induced oxidative stress, cytotoxicity and genotoxicity in human liver carcinoma (HepG2) cells. Environmental toxicology, United States, 25(3), 221-226, abril 2010.
Palacio Betancur I, Palacio Baena JA, Camargo Guerrero M. Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia. Actualidades Biológicas, Colombia, 31(90), 67-77, junio 2009
Ribeiro LR. Teste do micronúcleo em medula óssea de roedores in vivo. Mutagênese Ambiental. Universidad luterana de Brasil. Canoas.173-178, 2003.
Rodriguez EM. Efectos letales y subletales de dos plaguicidas sobre las especies Uca uruguayensis y Chasmagnathus granulata (Cangrejal Bonaerense). 1991. Tesis (Doctorado) Universidad Nacional de Buenos Aires, Argentina, 1991.
Sawhney AK, Johal MS. Erythrocyte Alterations Induced by Malathion in Channa punctatus (Bloch). Bulletin of Environmental Contamination & Toxicology, United States, 64:398-405, 2000
Schmid W. The micronucleus tests. Mutation Research/Environmental Mutagenesis and Related Subjects. Netherlands 31: 9-15, febrero 1975.
Siu WHL, Mak E, Jia C, D Luca-Abbott SB, Richardson BJ, Lam PKS. Micronucleus induction in gill cells of green-lipped mussels (Perna viridis) exposed to mixtures of polycyclic aromatic hydrocarbons and chlorinated pesticides. Environmental Toxicology and Chemistry: An International Journal, United States, 23(5), 1317-1325, noviembre 2009.
Sorsa M, Autio K, Demopoulos NA, Järventaus H, Rössner P, Šrám RJ, Stephanou G, Vlachodimitropoulòs D. Human cytogenetic biomonitoring of occupational exposure to 1,3-butadiene. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Netherlands, 309(2), 321-326, septiembre 1994.
Tanaka T, Shimizu N.Induced detachment of acentric chromatin from mitotic chromosomes leads to their cytoplasmic localization at G1 and the micronucleation by lamin reorganization at S phase. Journal of Cell Science, United Kingdom, 113(4), 697-707, febrero 2000.
Villamizar NTC, Orozco L J, & Melendez IG. Mutagenicidad de aguas antes y después de clorar en la planta de potabilización Empopamplona. Bistua: Revista de la Facultad de Ciencias Básicas, Colombia, 12(2), 03-13, junio 2014.
Vryzas Z, Vassiliou G, Alexoudis C, Papadopoulou-Mourkidou E. Spatial and temporal distribution of pesticide residues in surface waters in northeastern Greece. Water research, United Kingdom, 43(1), 1-10, enero 2009.
Walton DG, Acton AB, Stich HF. DNA repair synthesis following exposure to chemical mutagens in primary liver, stomach and intestinal cells isolated from rainbow trout. Cancer Research, United States, 44(3), 1120-1121, marzo 1984.
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