Isolation and morphological identification of fungi from “Solanum elaeagnifolium” green compost
DOI:
https://doi.org/10.34188/bjaerv8n1-054Palavras-chave:
Solanum elaeagnifolium, green compost, mycofloraResumo
The present study is a research of the quantitative and qualitative composition of mycoflora of a green compost, aged of 06 months and mesophilically produced from plant debris of an undesirable plant “Solanum elaeagnifolium“. Fungi were isolated on PDA medium and incubated at different temperatures “22°C, 30°C and 45°C”. The results has showed that the fungi concentration varies between 2,2 x105 and 6,24 x105 CFU/g of dried green compost according to the incubation’s temperature. Thirty species of fungi belonging to 10 genera were isolated and identified. The 03 principal genuses are: Aspergillus, Penicillium and Trichoderma, signifying that are the major representative of fungi community of “Solanum elaeagnifolium” green compost. The presences of a high concentration of antagonist species and the absence of pathogenic species witnessed the maturity of the green compost and make it as a good organic amendment for plant culture for stimulate their growth and to protect them from soil pathogens. The inventory of the mycoflora of this compost was necessary to determine its quality and the most appropriate application of them as a biological control agent against several plants’ pathogens fungi.
Referências
Albiach H, Canet R, Pomares F and IngelmoF. Microbial biomass content and enzymatic activities after the application of organic amendments to a horticultural soil. Bioresource Technology, 75, 2000.
Anastasi A, Varese GC, and Marchisio VF. Isolation and identification of fungal communities in compost and vermicompost. Mycolology, 97 (1): 33-44, 2005.
Beaulieu R, López-Mondéjar R, Tittarelli F, Ros M and Pascual JA. qRT-PCR quantification of the biological control agent Trichoderma harzianum in peat and compost-based growing media. Bioresources Technology, 102 (3): 2793-2798, 2011.
Beffa T, Staib F, Fisher JL, Lyon PF, Gumowski P, Marfenina OE, Dunoyergeindre S, Georgen F, Roch-Susuki R, Gallaz L, Latge P. Mycological control and surveillance of biological waste and compost. Medicinal Mycology, 36: 137-145, 1998.
Boulter JI, Boland GJ, Trevors JT. Compost: A Study of the developmental process and end-product potential for suppression of turf grass diseases. World Journal of Microbiology and Biotechnology, 16: 115-135, 2000.
Camozzi R. Tea has helpful microbes by billions. The register-Guard. Eugene, Oregon, USA., pp.2, 2003.
Daami-Remadi M H, Jabnnoun-Khiareddine F, Ayed K, Hibar IEA, Znaidi and El Mahdjoub M. In vitro and in vivo evaluation of individually compost fungi for poato Fusarium dry rot biocontrol. Journal of Biology and Science, 6: 572-580, 2006.
Domsch KH, Gams W and Anderson TH. Compendium of Soil Fungi, vol. 1, Academic Press, London, UK, 1980.
Dutruc-Rosset G. Techniques analytiques et de contrôle (Codex oenologique). Partie microbiologique. Office International de la Vigne et du Vin., pp. 23, 2003.
Eggen T, Sveum P. Decontamination of aged creosote polluted soil: the influence of temperature, white rot fungus Pleurotus ostreatus and pretreatment. International Biodeterioration and Biodegradation, 43: 125-133, 1999.
Eida Ibrahim A, Mohamed F, Matter F, Abd El Zaher H. Isolation and characterization of cellulolytic and hemicellulolytic fungi from salt affected soils and compost. Journal of Innovations in Pharmaceutical and Biological Sciences (JIPBS), 3(4): 164-170, 2016.
Grisselle E, Pérez-Sánchez María Del Carmen, Librán-Salas Lydia I, Rivera-Vargas y Myrna Alameda-hozada. Identification of fungi in municipal sewage sludge compost and in peat. Journal of Agriculture University PR, 98 (2) :179-193.revistas.upr.edu soil microbial communities to compost amendments. Soil Biol. Biochem., 38,460-470, 2014.
Khattabi N. Aptitude antagoniste de Trichoderma vis-à-vis de Sclerotium rolfsii, agent responsable de la pourriture racinaire de la betterave à sucre dans les Doukkala, et perspectives de son utilisation pratique en lutte biologique. Thèse de Doctorat d’Etat Es-Sciences. Université Cadi Ayyad, Faculté des Sciences Semlalia, Marrakech,pp.106, 2004.
Kiffer E, Morelet M. Les Deuteromycetes. Classification et cle´s d’identification generique. Paris, France: INRA Editions, pp.306, 1997.
Hoitink HAJ and Boehm MJ. Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Annual Review of Phytopathology, 37: 427-446, 1999.
Kastner M, Mahro B. Microbial degradation of polycyclic aromatic hydrocarbons in soils affected by the organic matrix of compost. Applied in Microbiology and Biotechnology, 44: 668-675, 1996.
Lalande R, Gagnon B and Simard RR. 1998. Microbial biomass C and alkaline phosphatase activity in two compost-amended soils. Canadian Journal of Soil Science, 78: 581–587, 1996.
Larbi M. Influence de la qualité des composts et de leurs extraits sur la protection des plantes contre les maladies fongiques. Thèse de Doctorat Es-Sciences, Faculté des Sciences de l’Université de Neuchâtel, Institut de Botanique, pp. 140, 2006.
Lazarovits G. Management of soil-borne plant pathogens with organic soil amendment: a disease control strategy salvaged from the past. Canadian Journal of Plant Pathology, 23: 1-7, 2001.
Levanon D and Pluda D. Chemical, physical and biological criteria for maturity in composts for organic farming. Compost Science Utility, 10: 339-346, 2002.
Lieckfeldt E, Samuels GJ, Nirenberg HI and Petrini O. A morphological and molecular perspective of Trichoderma viride: is it one or two species? Applied in Environmental Microbiology, 65(6): 2418-2428, 1999.
Masciandaro G, Ceccanti B and Garcia C. In situ vermicomposting of biologicalal sludges and impacts on soil quality. Soil Biology and Biochemistry, 32: 1015-1024, 2000.
Miller FC. Composting of Municipal Solid Waste and its Components. In: Palmisano AC, Barlaz MA, eds. Microbiology of Solid Waste. CRS Press: 115-154, 1996.
Minussi RC, Moraes SG, Pastore GM, Dura`n N. Biodecolorization screening of synthetic dyes by four white-rot fungi in a solid medium: possible role of siderophores. Letter Applied Microbiology, 33: 21-25, 2001.
Mouchacca J. Thermophilic fungi: Biodiversity and taxonomic status, Crypt. Mycology, 18 : 1969, 1997.
Mouria B, Ouazzani-Touhami A, Douira Al. Isolement et identification de la mycoflore du compost des déchets urbains solides. Revue « Nature & Technologie ». C- Sciences de l'Environnement, 9 : 13-28, 2012.
Pascual JA, Garcia C and Hernandez T. Lasting microbiological and biochemical effects of the addition of municipal solid waste on an arid soil. Biology and Fertility of Soils, 30: 1-6, 1999.
Peters S, Koschinsky S, Schwieger F and Tebbe C. Succession of microbial communities during hot composting as detected by PCR-Single-Strand-Conformation-Polymorphism-Based genetic profiles of Small-Subunit rRNA genes. Applied in Environment Microbiology, 66: 930-936, 2000.
Pitt JI. A laboratory guide to common Penicillium species. 3. ed. Victoria: Food Science Australia ,187, 2000.
Rapilly F. Les techniques de mycologie en pathologie végétale. Annales des Epiphytes. INRA. 19, pp. 102, 1968.
Riachi K. Compostage d'ordures ménagères et de déchets verts. Flore fongique et risques sanitaires potentiels. Thèse de doctorat. Grenoble, pp.200, 1998.
Sparling GP, Fermor TR, Wood DA. Measurement of the microbial biomass in composted wheat straw, and the possible contribution of the biomass to the nutrition of Agaricus bisporus. Soil Biological and Biochemistry, 14:609-611, 1982.
Souza Dias E, Guimarães SE, Siqueira FG, Silva R and Batista LR. Allergenic and toxigenic fungi in the compost of cultivation of Agaricus brasiliensis. Scientia Agraria, Curitiba, 10 (6) : 507-511, 2009.
Straatsma G, Samson RA. Taxonomy of Scytalidium thermophilum, an important thermophilic fungus in mushroom compost. Mycology Research, 97:321-328, 1993.
Tuiter G, Szczech M and Bollen GJ. Suppression of Rhizoctonia solani in potting mixtures amended with compost made from organic household waste. Phytopathology, 88:764-773, 1998.
Watanabe T. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species, Third Edition (Mycology) (3rd ed.). CRC Press, 2010.
Widmer TL, Graham, JH and Mitchell DJ. Composted municipal solid wastes promote growth of young citrus trees infested with Phytophtora nicotianae. Compost Science and Utilization, 7: 6-16, 1999.
Wiegant WM. A simple method to estimate the biomass of thermophilic fungi in composts. Biotechnology Techniques, 5:421-426, 1992.
Witfeld F, Begerow D and Guerreiro MA. Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil. Mycologicalal Progress, 20: 325-339, 2021.
Witter E and Lopez Real J. Nitrogen losses during the composting of sewage sludge, and the effectiveness of clay soil, zeolite, and compost in adsorbing the volatilized ammonia. Biological Wastes, 23: 279-294. 1998.
Yousofvand Z, Jamali S and Khateri H. Isolation and identification of thermotolerant and thermophilic fungi from soil and compost in Kermanshah province, Iran. Rostaniha, 21(1): 79-95, 2020.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
- O conteúdo dos artigos é de responsabilidade exclusiva dos autores.
- É permitida a reprodução total ou parcial do conteúdo dos artigos, desde que citada a fonte.
- Artigos com plágio serão recusados, e o autor do plágio perderá o direito de publicar nesta revista.
- Os nomes e endereços informados nesta revista serão utilizados exclusivamente para os serviços prestados por esta publicação e não estão disponíveis para outros fins ou a terceiros.
- Depois de enviar os artigos, os autores cedem os direitos autorais de seus artigos ao BJAER. Se você se arrepender da submissão, o autor tem o direito de solicitar ao BJAER que não publique seu artigo. Porém, essa solicitação deve ocorrer até dois meses antes da divulgação do número que o artigo será publicado.
- O BJAER usa a licença Creative Commons CC BY. Informações sobre esta licença podem ser encontradas em: https://creativecommons.org/licenses/by/3.0/br/
Brazilian Journal of Animal and Environmental Research (BJAER) se reserva o direito de efetuar nos originais alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua, respeitando, porém, o estilo dos autores. Os trabalhos finais não serão enviadas aos autores.
