Comparative analysis of phenolics, flavonoids, antioxidant, anti-inflammatory and antimecrobial potential of methanolic and aqueous extracts from bunium incrassatum tubers
DOI:
https://doi.org/10.34188/bjaerv8n1-046Palavras-chave:
Bunium incrassatum, antioxidant activity, anti-inflammatory activity, antibacterial activity.Resumo
This study investigates the comparative biological activity and chemical composition of aqueous and hydromethanolic extracts of Bunium incrassatum tubers, a plant with traditional medicinal uses. Both extracts were evaluated for their antioxidant, anti-inflammatory and antimicrobial activities. Antioxidant activity was measured by DPPH scavenging assays. In vitro anti-inflammatory activity using bovine serum albumin technique. While the antimicrobial activity was investigated using the disc diffusion assay against four Gram-positive and Gram-negative bacteria and three fungi.
The total phenol and flavonoid content showed that the methanolic extract had a significantly higher total phenolic content (167.4±3. 53 μg GAE/mg E) and flavonoid content (36.27± 2.18 μg QE/mg E) of than aqueous extracts. The antioxidant activity revealed that methanolic extract of B. incrassatum demonstrated higher antioxidant activity (IC50=1.15 ± 0.02 mg/mL). In contrast, it is the aqueous extract that has shown the most important anti-inflammatory activities to those of standard (sodium diclofenac).The study of antimicrobial activity demonstrated that the extracts showed a modest antibacterial and antifungal activity. Both extracts showed antibacterial activity, but the methanolic extract exhibited a higher zone of inhibition, especially against Staphylococcus aureus and Pseudomonas aeruginosa. This comparative analysis provides insights into the effectiveness of different solvent-based extractions for harnessing the therapeutic properties of Bunium incrassatum.
Referências
Alhakmani, F., Kumar, S., Khan, S.A. (2013). Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed, 3(8), 623-7.
Aiouaz, M., Bitam, A. (2022). Bunium incrassatum Bois. Batt. Trab. (Talghouda) in the improvement of thyroid tissue damages in female rats. Journal of Fundamental and Applied Pharmaceutical Science, 2(2), 92-107.
Amraoui, N., Boutefaha, Z., Belattar, N., & Arrar, L. (2023). Analgesic and antiinflammatory activities of the aqueous root extract of Algerian Bunium incrassatum. Archives of Biological Sciences, 76(00), 42-42.
Bahorun, T., Gressier, B., Trotin, F., Brunete, C., Dine, T., Vasseur, J., Gazin, J.C., Berroukeche, F., Attoui, N., Toul, F., Ziane, M., Soulimane, N.M., & Merzouk, H. (2022). Investigation of antioxidant and anti-hemolytic properties of Algerian Bunium incrassatum tubers and their effects as diet on histological and biochemical parameters of normal Wistar rats. Asian J Agric & Biol. 2022(1), 202007391.
Baydar, H., Sağdiç, O., Özkan, G., Karadoğan, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food control, 15 (3), 169-172.
Benhammou, N., AtikBekkara, F., KadifkovaPanovska, T. (2009). Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. C. R. Chimie. 12: 1259–1266.
Bouhalit, S,. Brahmi, N. (2021). Ameliorating Role of Ulva lactuca extract against Lithium neurotoxicity. J Biores Manag, 8(4), 121-134.
Bousetla, A., Zellagui, A., Derouiche, K., Rhouati, Salah. (2015). Chemical constituents of the roots of Algerian Bunium incrassatum and evaluation of its antimicrobial activity. Arabian Journal of Chemistry. 8(3), 313-316.
El Kolli, H., Laour, H., El Kolli, M. (2017). Chemical composition and biological activities of the essential oils and the methanolic extracts of Bunium incrassatum and Bunium alpinum from Algeria. Journal of The Chilean Chemical Society, 62, 3335-3341.
Griffin, S.G., Wyllie, S.G., Markham, J.L. and Leach, D.N. (1999). The role of structure and molecular properties of terpenoids in determining their antimicrobial activity, Flavour Fragr. J, 14: 322-332.
Habibur, R., ChinnaEswaraiah, M., Dutta, A. M. (2015). In-Vitro anti inflammatory and anti-arthritic activity ofOryza sativa Var. Joha Rice (An Aromatic Indigenous Rice of Assam). American-Eurasian J Agric Environ Sci; 15 (1): 115-21.
Mansouri, A., Embarek, G., Kokkalou, E., Kefalas, P. (2005). Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemist.. 89 411-420.
Molehin, O.R., Adefegha, S.A. (2014) Comparative study of the aqueous and ethanolic extract of Momordica foetida on the phenolic content and antioxidant properties. Int Food Res J, 21(1), 401–405
Nassima, B.G., Khouloud, B., Nour El Houda, H.A., Hena, A., & Fatima Zahra, H. (2022). Antioxidant and in vitro anti-inflammatory activities of the crude extracts of Bunium pachypodum P.W. Ball (Apiales Apiaceae) tubers from Algeria. Biodiversity Journal, 2022,13 (4), 969–980
Nivetha. B, Rahmathunisha. A, Lokeshwari. K, Kumaresan. A, Sai Nikkitha. K.V, Yeseshivi. L, Janani. R. (2022). Efficacy of Nanocurcumin with application of Iontophoresis on Inflammatory arthritis patients. Research J. Pharm.and Tech , 15(2),825-829.
Oyaizu. M. (1986). Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nat.44 . 307-315.
Pinkas, M., Luycky, M., Gazin, M. (1996). Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneim. Forsch. 46 1086-1089.
Quezel, P., Santa, S. Nouvelle flore de l'Algérie et des régions désertiques méridionales. C.N.R.S. éd., Paris, 2 vol. 1170 pp
Sachin, S., Archana, S., Juvekar, R., Gambhire, M. N. (2010). In¬vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci, 2(1),146.
Salehi, P., Mohammadi, F., Asghari, B. (2008). Seed essential oil analysis of Bunium persicum by hydrodistillation-headspace solvent. Chemistry of Natural Compounds, 44 , 111-113.
Senigra M., Oum Elkhair RL, Rezkallah C., Ammar H., Ghemam A.D. (2024). Identification, quantification, and antioxidant capacity of active compounds in Fagonia cretica organic extracts. Brazilian Journal of Animal and Environmental Research, 7(4),1-15.
Shahsavari, N., Barzegar, M., Sahari, M.A. (2008). Antioxidant activity and chemical characterization of essential oil of Bunium persicum. Plant Foods for Human Nutrition 63, 183–188.
Sharififar F., Yassa N., Mozaffarian V. (2010). Bioactivity of major components from the seeds of Bunium persicum (Boiss.) Fedtch. Pak J Pharm Sci, 23, 300-4.
Shimanda, K., Fajukawa, K., Yahara, K., Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soyabean oil in cyclodextrin emulsion. J Agric Food Chem, 40(6), 945– 948.
Sweetha, G., Sangeetha, B., Prabhu, S. (2013). A review on Curcumin nanoparticles and its controlled delivery to treat degenerative diseases. Asian J. Pharm. Tech, 3(4), 218-222.
Toul, F., Djendar, A. (2023). Comparison of Major Bioactive Components of Various Solvent Extracts from Algerian Bunium incrassatum Umbellules and Antioxidant Activity. Journal of the Turkish Chemical Society Section A: Chemistry, 10(2), 505-512.
Toul, F., Djendar, A., Seladji, M., & Berroukeche, F. (2022). Phytochemical profiling and evaluation of antioxidant activity of different solvent extracts from Algerian Bunium incrassatum seeds’. Journal of the Turkish Chemical Society Section A: Chemistry.
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