Evaluation of the therapeutic effect of Phillyrea angustifolia extract in cadmium-intoxicated rats

Autores

  • Abdellkrim Bennacer
  • Miloud Slimani
  • Djallal Eddine Houari Adli
  • Mostapha Brahmi
  • Dallal Kourat
  • Wafaa Arabi
  • Kaddour Ziani
  • Khaled Kahloula

DOI:

https://doi.org/10.34188/bjaerv8n1-011

Palavras-chave:

Phillyrea anguistofolia, cadmium, biochemical analyses, histological analyses, liver

Resumo

The study aims to investigate the impact of Cadmium (Cd) exposure on the liver of young Wistar rats and to evaluate the effectiveness of treatments with phillyrea anguistifolia aqueous extract in mitigating the damage caused by this chemical toxin. The plant extract was extracted via infusion and maceraion, resulting in yields of 10.9% for the infusion and 11 % for the maceration. Acute toxicity from Cadmium was induced by administering an oral dose of 20 mg/kg over 35 days. In rats exposed to Cd, there were significant increases in hepatic markers such as AST and ALT and ALP in the blood, alongside a inecrease in Glucose levels and urea and creatinin. Additionally, the liver showed signs of tissue damage. Treatment with Phillyra angusistifolia significantly alleviated the biochemical alterations, as well as the histological and cellular damage observed in the liver tissue.

Referências

AHMED, H. S.; MOHAMED, E. I. A.; AMIN, E.; et al. Phytochemical investigation and anti-inflammatory potential of Atriplex leucoclada Boiss. BMC Complement Med Ther, 2023; 23:464. [DOI:10.1186/s12906-023-04281-5]

ALI, M. A.; ALI, A. A.; ABDULRAHMAN, F. I.; ALI, H. M.; ALI, M. A. The role of antioxidants in ameliorating cadmium-induced oxidative stress in rats. Biol Trace Elem Res, 2021; 199(1):1-10. [DOI:10.1007/s12011-020-02284-0]

ALLAMEH, A.; NIAYESH-MEHR, R.; ALIARAB, A.; SEBASTIANI, G.; PANTOPOULOS, K. Oxidative Stress in Liver Pathophysiology and Disease. Antioxidants, 2023; 12:1653. [DOI:10.3390/antiox12091653]

ALOTHAID, H.; AL-ANAZI, M. R.; AL-QAHTANI, A. A.; COLAK, D.; YUSUF, A.; ALDUGHAIM, M. S.; MAHZARI, A. M.; HABIBULLAH, M. M.; ALARIFI, S.; ALKAHTANI, S.; AL-QAHTANI, A. A. Exposure to Cadmium Telluride Quantum Dots and Gene Expression Profile of Huh-7 Hepatocellular Carcinoma Cell Line. Dose Response, 2023; 21(3):15593258231185457. [DOI:10.1177/15593258231185457]

ANDRIOPOULOS, V.; GKIONI, M. D.; KOUTRA, E.; MASTROPETROS, S. G.; LAMARI, F. N.; HATZIANTONIOU, S.; KORNAROS, M. Total Phenolic Content, Biomass Composition, and Antioxidant Activity of Selected Marine Microalgal Species with Potential as Aquaculture Feed. Antioxidants, 2022; 11:1320. [DOI:10.3390/antiox11071320]

ANSES. Opinion and Collective Expert Report, 2012; Toxicity Reference Value for Cadmium and Its Compounds. Maison-Alfort, France.

BOUFISSIOU A, KADI I, BENAMER-AISSA B, BOUSSOUSSA H, HARRAT M, YOUSFI M. In vitro study of the antioxidant, sun factor protection, antimicrobial, and antifungal activities with molecular docking of methanolic extracts from leaves and fruit of Phillyrea angustifolia L. 3 Biotech. 2024 Feb;14(2):41. doi: 10.1007/s13205-023-03877-5. Epub 2024 Jan 17. PMID: 38261869; PMCID: PMC10794656.Chloride Induced Alteration of Rat’s Renal Histopathology.

BRAHMI M.; MELLALI S.; ARABI W.; ZIANI K.; KAHLOULA K.; SLIMANI M.; (2024). Exploration of the antioxidant and anti-diabetic activity in vitro of ethanolic extracts of phillyrea latifolia, phillyrea angustifolia, and spirulina. 10.48047/AFJBS.6.15.2024.12243-12252. Histopathology.

CHAVES, N.; SANTIAGO, A.; ALÍAS, J. C. Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used. Antioxidants, 2020; 9:76. [DOI:10.3390/antiox9010076]

DONG, X.; DING, A.; HU, H.; XU, F.; LIU, L.; WU, M. Placental Barrier on Cadmium Transfer from Mother to Fetus Related to Pregnancy Complications. Int J Women’s Health, 2023; 179-190. [DOI:10.2147/IJWH.S393067]

GABR, S.; ALGHADIR, A.; GHONEIM, G. Biological activities of Ginger against Cadmium-Induced Renal Toxicity. Saudi J Biol Sci, 2017; 26:10-16. [DOI:10.1016/j.sjbs.2017.08.008]

GENCHI, G.; SINICROPI, M. S.; LAURIA, G.; CAROCCI, A.; CATALANO, A. The Effects of Cadmium Toxicity. Int. J. Environ. Res. Public Health, 2020; 17(11):3782. [DOI:10.3390/ijerph17113782]

GOMEZ-QUIROZ, L.; SOUZA, A.; MARTÍNEZ-FLORES, K.; ORTIZ, L.; GUTIÉRREZ-RUIZ, M. C. Liver and Cadmium Toxicity. J Drug Metabol Toxicol, 2012; 5:10.4172/2157-7609.S5-001. [DOI:10.4172/2157-7609.S5-001]

GUAN, H.; LUO, W.; BAO, B.; CAO, Y.; CHENG, F.; YU, S.; FAN, Q.; ZHANG, L.; WU, Q.; SHAN, M. A Comprehensive Review of Rosmarinic Acid: From Phytochemistry to Pharmacology and Its New Insight. Molecules, 2022; 27:3292. [DOI:10.3390/molecules27103292]

GULCIN, I.; ALWASEL, S. H. DPPH Radical Scavenging Assay. Processes, 2023; 11:2248. [DOI:10.3390/pr11082248]

HARBONE, J. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. New York, 1998; ISBN: 0-41257260-5.

LAWAG, I. L.; NOLDEN, E. S.; SCHAPER, A. A. M.; LIM, L. Y.; LOCHER, C. A Modified Folin-Ciocalteu Assay for the Determination of Total Phenolics Content in Honey. Appl. Sci., 2023; 13:2135. [DOI:10.3390/app13042135]

MANTUR, V.; GOUDAR, S.; DAS, K. Amelioretic Effect of Black Tea Extract (BTE) on Cadmium Chloride Induced Alteration of Rat’s Renal Histopathology.

MARRERO, A. D.; QUESADA, A. R.; MARTÍNEZ-POVEDA, B.; MEDINA, M. Á. Anti-cancer, anti-angiogenic, and anti-atherogenic potential of key phenolic compounds from virgin olive oil. Nutrients, 2024; 16:1283. [DOI:10.3390/nu16091283]

MASSÁNYI, P.; MASSÁNYI, M.; MADEDDU, R.; STAWARZ, R.; LUKÁČ, N. Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs. Toxics, 2020; 8(4):94. [DOI:10.3390/toxics8040094]

MELIANA, A.; RATNANI, A. H. P.; HASANATULUDHHIYAH, N.; RAHNIAYU, A.; MASTUTIK, G.; RAHAJU, A. S. Protective Effect of Olive Leaf (Olea europaea L.) Extract Against Chronic Exposure of Liver and Kidney Tissues of Wistar Rats to Aluminum Chloride. J Herbmed Pharmacol., 2024; 13(2):333-341. [DOI:10.34172/jhp.2024.49313]

NAIR, A. R.; DEGHESSELLE, O.; SMEETS, K.; VAN KERKHOVE, E.; CUYPERS, A. Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)? Int J Mol Sci, 2013; 14:6116-6143. [DOI:10.3390/ijms14036116]

OOSTERWIJK, M. M.; HAGEDOORN, I. J. M.; MAATMAN, R. G. H. J.; BAKKER, S. J. L.; NAVIS, G.; LAVERMAN, G. D. Cadmium, Active Smoking, and Renal Function Deterioration in Patients with Type 2 Diabetes. Nephrology Dialysis Transplantation, 2023; 38(4):876–883. [DOI:10.1093/ndt/gfac270]

ORR, S. E.; BRIDGES, C. C. Chronic Kidney Disease and Exposure to Nephrotoxic Metals. Int. J. Mol. Sci., 2017; 18(5):1039. [DOI:10.3390/ijms18051039]

PLASKOVA, A.; MLCEK, J. New Insights of the Application of Water or Ethanol-Water Plant Extract Rich in Active Compounds in Food. Front. Nutr., 2023; 10:1118761. [DOI:10.3389/fnut.2023.1118761]

PONHAIBOON J.; KRONGRAWA W.; AUNG W.; CHINATANGKUL N.; LIMMATVAPIRAT C. (2023). Advances in Natural Product Extraction Techniques, Electrospun Fiber Fabrication, and the Integration of Experimental Design: A Comprehensive Review. Molecules, 28(13), 5163.[doi.org/10.3390/molecules28135163]

QU, F.; ZHENG, W. Cadmium Exposure: Mechanisms and Pathways of Toxicity and Implications for Human Health. Toxics, 2024; 12(6):388. [DOI:10.3390/toxics12060388]

RAHIMZADEH, M. R.; RAHIMZADEH, M. R.; KAZEMI, S.; MOGHADAMNIA, A. A. Cadmium Toxicity and Treatment: An Update. Caspian J Intern Med., 2017; 8(3):135-145. [DOI:10.22088/cjim.8.3.135]

REGAD, N., ADLI, D. E. H., HALLA, N., BRAHMI, M., KADDOUR, Z., ARABI, W., SLIMANI, M. (2024). Effect of Ononis angustissima extract on the Aluminium alterations in intestinal histology. Brazilian Journal of Animal and Environmental Research, 7(4), e75424-e75424.

RIZALDY, D.; RAMADHITA, N.; NADHIFA, T.; FIDRIANNY, I. Evaluation of In Vitro Antioxidant Activities of Mangosteen (Garcinia mangostana L.). Pharmacognosy Journal, 2022; 14:633-640. [DOI:10.5530/pj.2022.14.82]

ROMANI, A.; BALDI, A.; MULINACCI, N.; et al. Extraction and Identification Procedures of Polyphenolic Compounds and Carbohydrates in Phillyrea (Phillyrea angustifolia L.) Leaves. Chromatographia, 1996; 42:571–577. [DOI:10.1007/BF02290294]

SATARUG, S.; GOBE, G. C.; VESEY, D.; PHELPS, K. R. Cadmium and Lead Exposure, Nephrotoxicity, and Mortality. Toxics, 2020; 8(4):86. [DOI:10.3390/toxics8040086]

SHRAIM, A. M.; AHMED, T. A.; RAHMAN, M. M.; HIJJI, Y. M. Determination of Total Flavonoid Content by Aluminum Chloride Assay: A Critical Evaluation. LWT, 2021; Volume 150:111932. [DOI:10.1016/j.lwt.2021.111932]

UMEMURA, T.; WAKO, Y. Pathogenesis of Osteomalacia in Itai-itai Disease. J Toxicologic Pathol, 2006; 19:69-74. [DOI:10.1293/tox.19.69]

WAGNER, H. Drogenanalyse, Dünschichtchromatographische Analyse von Arzneidrogen. Springer Verlag Berlin Heidelberg New York, 1983; 522 p.

YAN, L.-J.; ALLEN, D. C. Cadmium-Induced Kidney Injury: Oxidative Damage as a Unifying Mechanism. Biomolecules, 2021; 11(11):1575. [DOI:10.3390/biom11111575]

YILDIZ DENIZ, G.; UTLU, N. Olive leaf extract (Olea europaea L.) restores liver functions from cadmium-induced liver injury. J Basic Health, 2023; 2(1):8-17.

Downloads

Publicado

2025-01-07

Como Citar

Bennacer, A., Slimani, M., Adli, D. E. H., Brahmi, M., Kourat, D., Arabi, W., Ziani, K., & Kahloula, K. (2025). Evaluation of the therapeutic effect of Phillyrea angustifolia extract in cadmium-intoxicated rats. Brazilian Journal of Animal and Environmental Research, 8(1), e76485. https://doi.org/10.34188/bjaerv8n1-011

Edição

v. 8 n. 1 (2025)

Seção

Artigos originais

Licença

  • O conteúdo dos artigos é de responsabilidade exclusiva dos autores.
  •  É permitida a reprodução total ou parcial do conteúdo dos artigos, desde que citada a fonte.
  •  Artigos com plágio serão recusados, e o autor do plágio perderá o direito de publicar nesta revista.
  •  Os nomes e endereços informados nesta revista serão utilizados exclusivamente para os serviços prestados por esta publicação e não estão disponíveis para outros fins ou a terceiros.
  •  Depois de enviar os artigos, os autores cedem os direitos autorais de seus artigos ao BJAER. Se você se arrepender da submissão, o autor tem o direito de solicitar ao BJAER que não publique seu artigo. Porém, essa solicitação deve ocorrer até dois meses antes da divulgação do número que o artigo será publicado.
  •  O BJAER usa a licença Creative Commons CC BY. Informações sobre esta licença podem ser encontradas em: https://creativecommons.org/licenses/by/3.0/br/

 

 

Brazilian Journal of Animal and Environmental Research (BJAER) se reserva o direito de efetuar nos originais alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua, respeitando, porém, o estilo dos autores. Os trabalhos finais não serão enviadas aos autores.