Therapeutic potential of vitamin B6 in preventing hematological, biochemical, and histopathological alterations in mercury-exposed rabbits
DOI:
https://doi.org/10.34188/bjaerv7n4-094Palavras-chave:
mercury, toxicity, vitamin B6, protective effect, male rabbitsResumo
This study aims to examine the potential protective role of vitamin B6 against mercury-induced damage in the kidneys and thymus.
A total of a twenty-four male rabbits were evenly divided into four groups: the control group, the Hg group (0.5 g HgCl2/kg body weight), the VitB6 group (5 ppm by gavage), and the Hg + VitB6 group (0.5 g HgCl2/kg body weight + 5 ppm VitB6 by gavage) HgCl2 was included into the standard diet on a daily basis.
The results indicated that exposure to HgCl2 for thirty days resulted in an elevation of white blood cell counts, along with significant decreases in platelet, erythrocyte, haematocrit, and haemoglobin levels. Moreover, plasma levels of urea and creatinine were increased in the HgCl2 group relative to the control group. An observable elevation in kidney malondialdehyde levels was noted, associated with a reduction in reduced glutathione levels and diminished activities of glutathione peroxidase and superoxide dismutase in the HgCl2 groups. The gavage by vitamin B6, whether administered alone or combined with HgCl2, enhanced all assessed parameters. Histological evaluations of the kidneys and thymus demonstrated substantial tissue modifications in the HgCl2 group, characterised by severe glomerular atrophy, tubular necrosis, dilatation of Bowman’s capsule, thymic cortex atrophy, and lymphocyte depletion. However, rabbits administered HgCl2 and vitamin B6 had a restored histological architecture in these organs.
Hgcl2 produces oxidative stress and modified biochemical markers, combined with the histological profiles of the thymus and kidneys. Nonetheless, the use of vitamin B6 has alleviated this toxicity.
Referências
Agarwal R, Goel SK, Chandra R, Behari JR. Role of vitamin E in preventing acute mercury toxicity in rat. Environ Toxicol Pharmacol. 2010 Jan;29(1):70-8. doi: 10.1016/j.etap.2009.10.003.
Ali, S., Hussain, S., Khan, R., Mumtaz, S., Ashraf, N., Andleeb, S., Shakir, HA., Tahir, HM., Khan, M.K.A., Ulhaq, M.( 2019).Renal Toxicity of Heavy Metals (Cadmium and Mercury) and Their Amelioration with Ascorbic Acid in Rabbits. Environmental Science and Pollution Research,(26), 3909-3920. DOI:10.1007/s11356-018-3819-8
Aslam, M.F., Majeed, S., Aslam, S., Irfan, J.A.( 2017). Vitamins: Key Role Players in Boosting Up Immune Response-A Mini Review. Vitamins & Minerals.(31),6(1). DOI: 10.4172/2376-1318.1000153
Aslanturk, A., Uzunhisarcikli, M., Kalender, S., & Demir, F. (2014). Sodium selenite and vitamin E in preventing mercuric chloride induced renal toxicity in rats. Food and chemical toxicology,(70),185-190. DOI:10.1016/J.FCT.2014.05.010
Balali, M.M., Naseri, K., Tahergorabi, Z., Khazdair, M.R., Sadeghi, M.( 2021).Toxic Mecanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium and Arsenic. Frontiers in Pharmacology, (13),643972. DOI: 10.3389/fphar.2021.643972
Barregard, L., Rekic, D., Horvat, M., Elmberg, L., Lundh, T., Zachrisson, O.( 2011) Toxicokinetics of Mercury after Long-Term Repeated Exposure to Thimerosal-Containing Vaccine.Oxford University Press on behalf of the Society of Toxicology,120(2),499-506. DOI:10.1093/toxsci/kfr009
Bernhoft, R.A.( 2012). Mercury Toxicity and Treatment: A Review of the Literature. Journal of Environmental and Public Health,DOI:10.1155/2012/460508
Bracci, M., Tomasetti, M., Malavolta, M., Bonacucina, V., Mocchegiani, E., Santarelli, L. (2008).L-arginine Reduces Mercury Accumulation in Thymus of Mercury-exposed Mice: Role of Nitric Oxide Synthase Activity and Metallothioneins. Industrial Health.,(46),567–574.DOI: 10.2486/indhealth.46.567
De Wit, J. E. (2011). The role of vitamin B6 in one-carbon metabolism, oxidative stress,immunesystem regulationandcarcinogenesis(Bachelor'sthesis).
Ekawanti, A., Krisnayanti, B.D.( 2015). Effect of Mercury Exposure on Renal Function and Hematological Parameters among Artisanal and Small-scale Gold Miners at Sekotong, West Lombok, Indonesia. J Health Pollut,5(9),25-32. doi: 10.5696/2156-9614-5-9.25. PMID: 30524774; PMCID: PMC6221489.
Elblehi, S.S., Hafez, M.H., El-Sayed, Y.S.(2019).L-Α-Phosphatidylcholine Attenuates Mercury-Induced Hepato-Renal Damage Through Suppressing Oxidative Stress and Inflammation.Environmental Science and Pollution Research,5(26),9333-9342. DOI:10.1007/s11356-019-04395-9
Ellman., GL.(1959). Tissue sulfhydryl groups. Arch Biochem Biophys,82:70. DOI: 10.1016/0003-9861(59)90090-6
Francis,Y.M.,Vijayakumar,J.,Raghunath,G.,Vijayalakshmi,S.,Sivanesan,S.,Vijayaraghavan, R., & Sukumar, E. (2020). Protective effect of Carica papaya leafextract against mercuric chloride-induced nephrotoxicity in wistar rats. PharmacognosyMagazine,16(70).DOI:10.4103/pm.pm_11_20
Ghanim-Rhyaf A. (2016).Pathogenetic Effects of Mercury Chloride in White Rats. Bas.J.Vet.Res,15(2),176-191. DOI: 10.3376/bvert.2016.124295
Giannopolitis, C.N., Ries, S.K.(1977). Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol,59(2),309-14. doi: 10.1104/pp.59.2.309.
Hasrat, J.A.(2015). Exposure to mercury and health risks The Impact of Mercury on Human Health and Environment. Academic Journal of Suriname,(6),608-614.
Hellmann, H., Goyer, A., Navarre, D.A.(2021).Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide. Molecules,(26),2446. Doi:10.3390/molecules26092446
Hsu, C.C., Cheng, C.H., Hsu,C.L., Lee, W.J., Huang, Y.C.(2015). Role of Vitamin B6 Status on Antioxidant Defenses, Glutathione, and Related Enzyme Activities in Mice with Homocysteine-Induced Oxidative Stress. Food & Nutrition Research,59(1),25702. doi: 10.3402/fnr.v59.25702
Huq, M.A., Awal, M.A., Mostofa, M., Ghosh, A., Das, A.R.(2008). Effects of Vitamin E and Vitamin C on Mercury Induced Toxicity in Mice. Progress. Agric,19(2), 93-100.
Islam, M.T., Talukder, A.K., Talukder, M., Amin, M.R., Alam, K.J., Haider, M.G.(2017). Mercury Intoxication in Rats: Iron and Vitamin B6 as A Potential Therapy.Iranian Journal of Toxicology,11(4), 19-26.DOI : 10.29252/arakmu.11.4.19
Jan, A. T., Ali, A., & Haq, Q. M. R. (2011). Glutathione as an antioxidant in inorganicmercuryinducednephrotoxicity. Journalofpostgraduatemedicine, 57(1),72-77.doi:10.4103/0022-3859.74298
Kosuda, L. L., Hannigan, M. O., Bigazzi, P. E., Leif, J. H., & Greiner, D. L. (1996).Thymus atrophy and changes in thymocyte subpopulations of BN rats with mercury-inducedrenal autoimmune disease.Autoimmunité,23(2),77-89. DOI: 10.3109/08916939608995331
Li, X., Yin, D., Yin, J., Chen, Q., Wang, R.(2014). Dietary selenium protect against redox-mediated immune suppression induced by methylmercury exposure. Food ChemToxicol, (72),169-77. DOI: 10.1016/J.FCT.2014.07.023.
Martoja, R., Martoja, M.(1967).Initiation aux Techniques de l’histologie Animale. Ed Masson and Cie, Paris,p3,45-346.
Masson, P., Ohllson, P., Bjorkhem, I.,(1981). Combined enzymaic-Jaffe’s method for Determinatin of Creatinine in Serum. Clin Chem,(27),18-2.doi.org/10.1093/clinchem/27.1.18
Miguel, M.C., Cazaux, N., Meder, A.R (2022). Creatinine: review of its usefulness and scope as a marker of kidney injury Brazilian Journal of Animal and Environmental Curitiba, v.5, n.3, p. 3071-3081, jul./set. DOI: 10.34188/bjaerv5n3-040
Moszczyński, P.(1997). Mercury compounds and the immune system: a review. Int J Occup Med Environ Health,10(3),247-58.
Moumen, Y. Benbott, A. Hamdouche, N. Abdennour, C. (2022). The role of vitamin C on defense system of domestic rabbit Oryctolagus cuniculus in mercury induced toxicity. Revue des BioRessources. 12 (1), 101-112.
Mumtaz , S., Ali , S., Khan, R., Andleeb, S., Ulhaq, M., Khan, M.A., Shakir, H.A. (2019).The protective role of ascorbic acid in the hepatotoxicity of cadmium and mercury in rabbits. Environ SciPollutRes Int,26(14),14087-14096. doi: 10.1007/s11356-019-04620-5.
Orr, S.E., Bridges, C.C.(2017).Chronic Kidney Disease and Exposure to Nephrotoxic Metals. Int J Mol Sci,18(5),1039. DOI: 10.3390/ijms18051039.
Paglia, D.E., Valentine, W.N. (1967). Studies on quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab Med,(70),158-69
Raeeszadeh, M., Moradi, M., Ayar, P., & Akbari, A. (2021). The antioxidant effect ofMedicago sativa L.(alfalfa) ethanolic extract against mercury chloride (HgCl2) toxicity inrat liver and kidney: an in vitro and in vivo study. Evidence-Based Complementary andAlternativeMedicine,2021(1), 8388002DOI: 10.1155/2021/8388002
Rakib, M.S., Sujan, K.M., Miah, M.A.(2021).Deleterious Effects Of Mercuric Chloride On Blood Biochemistry, Liver And Kidney Histology In Female Albino Mice.Journal of Agriculture, Food and Environment,2(2), 18-23. Doi:10.47440/JAFE.2021.2204
Rana, M.N., Tangpong, J., Rahman, M.M.(2018).Toxicodynamics of Lead, Cadmium, Mercury and Arsenic- induced kidney toxicity and treatment strategy: A mini review. Toxicol Rep,(5),704-713. doi: 10.1016/j.toxrep.2018.05.012.
Rao, M.V., Sharma, P.S.(2001). Protective effect of vitamin E against mercuric chloride reproductive toxicity in male mice. Reprod Toxicol,15(6),705-12. doi: 10.1016/s0890-6238(01)00183-6.
Rastegar, A. M., Bidadkosh, A., Vahdani, R., Eini, F., & Azadi, S. (2014). Vitamin B 6dose-dependently ameliorates renal hemodynamic toxicity of cisplatin in rat model ofnephrotoxicity:thehistopathologicandbiochemicalfindings. ComparativeClinicalPathology,23, 703-708.DOI: 10.1007/s00580-012-1674-4
Rhyaf,A.G.(2016).PathogeneticEffectsOfMercury ChlorideInWhiteRats. BasrahJournalof VeterinaryResearch,15(2), 176-191.
Ribarov, S.R. Benov, L.C. Benchev, I.C. (1983). On the mechanism of mercury induced hemolysis. General Physiology and Biophysics. 2(2), 81–84.
Rojas-Franco, P., Franco-Colín, M., Torres-Manzo, A.P., Blas-Valdivia, V., Thompson-Bonilla, M.D.R., Kandir, S., Cano-Europa, E.(2019). Endoplasmic reticulum stress participates in the pathophysiology of mercury-caused acute kidney injury. Ren Fail,41(1),1001-1010. doi: 10.1080/0886022X.2019.1686019.
Salazar-Flores ,J., Torres-Jasso, J.H., Rojas-Bravo, D., Reyna-Villela, Z.M., TorresSánchez, E.D.(2019).Effects of Mercury, Lead, Arsenic and Zinc to Human Renal Oxidative Stress and Functions: A Review.Journal of Heavy Metal Toxicity and Diseases,4(1),1-16.doi: 10.21767/2473-6457.10027
Saljooghi, A.S.,Mendi, F.D. (2013). The effect of mercury in iron metabolism in rats. Clinical Toxicology, 3(5), 1-5. DOI: 10.4172/2161-0495.S3-006
Salvator, N.I., Finbarrs-Bello, E., Ikemefuna, O.I., Ejuiwa, M.C., Ugwu, V.(2023).Protective Roles of Quercetin, Vitamin C and Pyridoxine on Lead Neurotoxicity via Enhanced Haematopoietic and Antioxidants Components. Global Online Journal of Academic Research (GOJAR), 2023 Jan-Fab;2(1)
Stach, K., Stach, W., Augoff, K.(2021).Vitamin B6 in Health and Disease. Nutrients,(13),3229. doi.org/10.3390/nu13093229
SVS Rana. Protection of Metal Toxicity by Melatonin - Recent Advances. EC Pharmacology and Toxicology. 2018 Jun 5; 6(8)
Uzunhisarcikli, M., Aslanturk, A., Kalender, S., Apaydin,FG., Bas, H.(2016). Mercuric chloride induced hepatotoxic and hematologic changes in rats: The protective effects of sodium selenite and vitamin E. ToxicolInd Health,32(9),1651-62. doi: 10.1177/0748233715572561.
Vianna, A.D.S., Matos, E.P., Jesus, I.M., Asmus, C.I.R.F., Câmara, V.M. (2019). Human exposure to mercury and its hematological effects: a systematic review. Cad Saude Publica,35(2),e00091618. doi: 10.1590/0102-311X00091618.
Yagi,K. (1976).A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med,15(2),212-6. doi: 10.1016/0006-2944(76)90049-1.
Zulaikhah, S. T., &Wibowo, J. W. (2018).The Effect of Tender Coconut Water on FreeRadicalDuetoMercuryExposure.InternationalJournalofPublicHealthScience(IJPHS),7(2), 102-106. DOI:10.11591/ijphs.v7i2.11849.
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