The genetic diversity of some North African sheep breeds using microsatellite markers

Autores

  • Yacine Ouinten
  • Sid Ahmed Aouadj
  • Bachir Medrouh
  • Imène Lafri
  • Dalila Ranebi
  • Semir Bechir Suheil Gaouar
  • Pavel Mikhailovich Klenovitskii
  • Walid Benchiha

DOI:

https://doi.org/10.34188/bjaerv7n4-075

Palavras-chave:

genetic, structure, diversity, microsatellite, Algeria

Resumo

The genetic structure and diversity of eight indigenous sheep breeds - Sidaoun, Rembi, Hamra, D’men, Ouled Djellal, Sardi, Berguia and Daraa- were identified by analyzing ten microsatellite loci in 183 unrelated individuals. Parameters such as allele diversity, observed heterozygosity, expected heterozygosity, unbiased expected heterozygosity, and genetic distance have been calculated. Additionally, wright F-statistics values (FIS, FIT and FST) were determined. The average number of alleles per locus was also estimated. Within these breeds, the mean number of alleles ranged from 12.60 in D’men to 17.80 in Ouled Djellal. Mean expected heterozygosity (He) varied between 0.83 in Sidaoun and 0.91 in Ouled Djellal. Inbreeding coefficient for all microsatellites is low, with a negative FIS = -0.014 ranging from -0.057 for INRA063 to 0.018 in McM42. The FST (mean genetic differentiation) value was found to be 0.062, indicating that only 6.2% of total genetic diversity is attributed to genetic differentiation among populations, while 93.8% of total genetic variance results from differentiation within each population. A phylogenetic tree based on genetic distance revealed three distinct groups: D'men/ Sidaoun, Ouled Djellal/ Rembi/ Berguia, and Sardi/ Daraa. However, the Hamra sheep breed appeared in a separate cluster.

This study has provided important information on the genetic resources of Hamra, Rembi, D'men, and Sidaoun sheep that justifies priority conservation measures. Our results confirm that the microsatellite markers used in this study are sufficiently polymorphic and can be successfully used to study the genetic diversity of Algerian sheep populations.

Referências

Abdelkader, A.A; Ata, N; Benyoucef, M.T; Djaout, A; Azzi, N; Yilmaz, O. (2018). New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers. Ital J Anim Sci, 17:38–48.

Agung, P.P; Saputra, F; Zein, M.S.A; Wulandari, A.S; Putra, W.P.B; Said, S. (2019). Genetic diversity of Indonesian cattle breeds based on microsatellite markers. Asian-Australasian J Anim Sci, 32:1-467.

Ahmed, Z; Babar, M.E; Hussain, T; Nadeem, A; Awan, F.I; Wajid, A. Genetic (2014). Diversity analysis of Kail sheep by using microsatellite markers. JAPS J Anim Plant Sci 1: 1-24.

Aouadj, S. A. (2009). Apport de la télédétection et SIG pour la cartographie des risques d'érosion hydrique du sol dans la région de Saida– (Western Algeria). These of engineering, Saida University, Tlemcen, Algeria.

Aouadj, S. A. (2021). Impact of ecological restoration techniques on the dynamics of degraded ecosystems in the Saida mountains: Case of the forests of Doui Thabet – (Western Algeria). PhD theses, Aboubakr Belkaïd University, Tlemcen, Algeria.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O. & Khatir, H. (2020). Impact of ecological restoration techniques on the dynamics of degraded ecosystems of the mounts of Saida: Case of the forests of Doui Thabet (West Algeria). Acta scientifica naturalis, 7 (2): 68-77.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). Impacts of anthropogenic pressure on the degradation of the forest of Doui Thabet (Saida, Western Algeria) in the context of the restoration. Acta scientifica naturalis, 7 (2): 68-78.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). Note on the orchids of mounts of Saida (Saida Western Algeria) in the context of the restoration. Eco. Env. & Cons, 26 (2): 37-45.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). The rare, endemic and threatened flora of the mountains of Saida (Algeria). Agrobiologia, 10(1), 86 – 98.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). Ethnobotanical Approach and Floristic Inventory of Medicinal Plants in the Doui Thabet Region (Saida-Western Algeria). PhytoChem & BioSub Journal, 14 (1): 92-104.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). Regional phytogeographic analysis of the flora of the Mounts of Saida(Algeria): evaluation-restoration report. Biodiversity Journal, 11 (1): 25-34.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2020). Ecological characterization and evaluation of the floristic potential of the forest of Doui Thabet (Saida Western Algeria) in the context of the restoration. Eco. Env. & Cons, 26 (1): 266-278.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2022). Preliminary study of the pre-germinative treatments of Juniperus oxycedrus L. and Pistacia lentiscus L. Res. Conserv, (67): 13-20, 2022.

Aouadj, S. A; Nasrallah, Y; Hasnaoui, O & Khatir, H. (2023). Floristic and ecological diagnostic of the Mounts of Saida in the context of ecological restoration: Assessment of six years of field research (2017-2022). Journal Concepts in Structural Biology & Bioinformatics (JSBB), 7 (1) : 1-42.

Aouadj, S. A; Degdag, H; Hasnaoui, O; Nasrallah, Y; Zouidi, M; Allame, A; Nouar, B. & Khatir, H. (2023). Contribution of G.I.S and Remote Sensing for the Risk Mapping of Soil Water Erosion at Saida Province (Western of Algeria). Advanced Research In Life Sciences, 7 (1) : 10 – 21.

Aouadj, S. A; Degdag, H; Hasnaoui, O; Nasrallah, Y; Zouidi, M; Allame, A; Nouar, B. & Khatir, H. (2023). New data on Orchid flora (Orchidaceae) in the Tell region of Saida (western of Algeria): Assessment of six years of field research (2017-2022). Advanced Research In Life Sciences, 8 (1): 10 – 21.

Barker, JSF; Tan, SG; Moore, SS; Mukherje, TK, Matheso, JL; Selvaraj, OS. (2001). Genetic variation within and relationships among populations of Asian goats (Capra hircus). J Anim Breed Genet, 118 :213–34.

Belkhir, K. (2004). GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Http//Www Genet Univ-Montp2 Fr/Genetix/Genetix Htm 2004.

Benchiha, W., Aouadj, S. A., Megharbi, A. and Tamret, A. (2024). Antioxidant activity and content of total phenols and flavonoids of aqueous extracts from leaves of Pistacia lentiscus L. Brazilian journal of animal and environmental research, 7 (4) : 1-15.

Cemal, I; Yilmaz, O; Ata, N. (2013). Analysis of genetic diversity in indigenous Çine Çaparı sheep under conservation by microsatellite markers. Studies, 5:1-6.

Chekkal, F; Benguega, Z; Meradi, S; Berredjouh, D; Boudibi, S; Lakhdari, F. (2015). Guide de caractérisation phénotypique des races ovines de l’Algérie. CRSTRA.

Chellig, R. (1992). Algerian sheep breeds, Office of University Publications, Algeria.(Races ovines algériennes, Office des publications universitaires, Algérie).

Chessa, B; Pereira, F; Arnaud, F; Amorim, A; Goyache, F; Mainland, I. (2009). Revealing the history of sheep domestication using retrovirus integrations. Science, 80: 324:1-532.

Crawford, AM; Cuthbertson, RP. (1996). Mutations in sheep microsatellites. Genome Res, 6:876–9.

Dávila, SG; Gil, MG; Resino-Talaván, P; Campo, JL. (2009). Evaluation of diversity between different Spanish chicken breeds, a tester line, and a White Leghorn population based on microsatellite markers. Poult Sci, 88:2518–25.

Devendra, C; McLeroy, GB. (1982). Goat and sheep production in the tropics. Longman.

Dorji, N; Duangjinda, M; Phasuk, Y. ( 2012). Genetic characterization of Bhutanese native chickens based on an analysis of Red Junglefowl (Gallus gallus gallus and Gallus gallus spadecieus), domestic Southeast Asian and commercial chicken lines (Gallus gallus domesticus). Genet Mol Biol, 35:603–9.

Earl, DA; VonHold,t BM. (2012). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour, 4:359–61.

Evanno, G; Regnaut, S; Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 14:2611–20.

FAO R. FAOSTAT. (2022). https://www.fao.org/faostat/en/#data/Q.

FAO, R. (2011). Molecular genetic characterization of animal genetic resources. FAO Anim Prod Heal Guidel.

Ferrah, A; Oubelli, MA. (2013). Algeria analysis of food, agriculture and water research and innovation priorities, needs and capacities. Alger Minist Agric Natl Inst Natl Agron Res Alger Wafira Proj.

Frankham, R; Briscoe, DA; Ballou, JD. (2002). Introduction to conservation genetics. Cambridge university press.

Gaouar, SBS; Da Silva, A; Ciani, E; Kdidi, S; Aouissat, M; Dhimi, L. (2015). Admixture and Local Breed Marginalization Threaten Algerian Sheep Diversity. PLoS One, 10: 13.

Gaouar; SBS; Lafri, M; Djaout, A; El-Bouyahiaoui, R; Bouri, A; Bouchatal, A. (2017). Genome-wide analysis highlights genetic dilution in Algerian sheep. Heredity, 118: 301.

Gene Calc. Polymorphic information content & Heterozygosity. (2022). https://gene-calc.pl/pic.

Gutiérrez-Espeleta ,GA; Kalinowski, ST; Boyce, WM; Hedrick, PW. (2000). Genetic variation and population structure in desert bighorn sheep: implications for conservation. Conserv Genet, 1:3–15.

Hammer, O; Harper, DAT; Ryan, PD. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron, 4:1-9.

Harkat, A; Benzid, R; Saidi, L. (2015). Features extraction and classification of ECG beats using CWT combined to RBF neural network optimized by cuckoo search via levy flight. 2015 4th Int. Conf. Electr. Eng., IEEE; 1–4.

Hoda, A; Marsan, PA.(2012). Genetic characterization of Albanian sheep breeds by microsatellite markers. Anal Genet Var Anim, 2:3–27.

Jakobsson, M; Rosenberg, NA. (2007). CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics, 23:1801–6.

Kalinowski, ST. (2004). Counting alleles with rarefaction: private alleles and hierarchical sampling designs. Conserv Genet, 5:539–43.

Kerzabi, R; Aouadj, S.A. (2024). Chemical Characterization and Biological Activity Assessment of Atriplex halimus Essential Oil: Exploring New Avenues in Pharmacology and Medicine. Brazilian journal of animal and environmental research, 7 (4) : 1-15.

Kevorkian, SEM; Georgescu, SE; Manea, MA; Zaulet, M; Hermenean, AO; Costache, M. (2010). Genetic diversity using microsatellite markers in four Romanian autochthonous sheep breeds. Rom Biotech Lett, 15: 5059–5065.

Lafri, I; Aouadj, S.A. (2024). Characterization of phycocyanin collected in Algeria. Brazilian journal of animal and environmental research, 7 (4) : 1-15.

Lafri, M; Ferrouk, M; Harkat, S; Routel, A; Medkouk, M; Dasilva, A. (2014). Caractérisation génétique des races ovines algériennes. Chentouf M, Lopez-Francos A, Bengoumi M, Gabina D(Éds) Technol Creat Transf Small Ruminants Roles Res Dev Serv Farmer Assoc Options Méditerranéennes (CIHEAM), Série A, 108:293–8.

Lahlou-Kassi, A; Berger, YM; Bradford, GE; Boukhliq, R; Tibary, A; Derqaoui, L. (1989). Performance of D’Man and Sardi sheep on accelerated lambing I. Fertility, litter size, postpartum anoestrus and puberty. Small Rumin Res, 2:225–39.

MATET. (2009). Quatrième rapport national sur la mise en œuvre de la convention sur la diversité biologique au niveau national.

Moula, N; Scippo, M-L; Douny, C; Degand, G; Dawans, E; Cabaraux, J-F. (2018). Performances of local poultry breed fed black soldier fly larvae reared on horse manure. Anim Nutr, 4:73–8.

Nasrallah, Y; Aouadj, S.A; Khatir, H. (2020). Impact of the exploitation of medicinal plants on biodiversity conservation in Saida El Bayadh regions, Algeria. Biodiv. Res. Conserv. 60: 11-22.

Nei, M.(1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 189:83–90.

Nei, M.(1972). Genetic distance between populations. Am Nat 1972;106:283–92.

Odjakova, T; Todorov, P; Radoslavov, G; Hristov, P. (2022). Microsatellite Genotyping of Two Bulgarian Sheep Breeds. Diversity, 14:210.

Ojango, JM; Mpofu, N; Marshall, K; Andersson-Eklund, L. (2011). Quantitative methods to improve the understanding and utilization of animal genetic resources. Anim Genet Train Resour Version, 3:1-2011.

Ouinten, Y; Klenovitski, PM; Nikichov, AA; Gladyr, EA. (2018). Genetic and morphological diversity in Algerian sheep breeds. Innov. Agric, 1 : 117–22.

Peakall, ROD; Smouse, PE. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes, 6:288–95.

Pritchard, JK; Stephens, M; Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics, 155:945–59.

Ren, A; Du, K; Jia, X; Yang, R; Wang, J; Chen, S-Y. (2019). Genetic diversity and population structure of four Chinese rabbit breeds. PLoS One, 4 : 1-100.

Sagne, J. (1950). L’Algérie pastorale, ses origines, sa formation, son passé, son présent, son avenir. imprimerie Fontana.

Sarson, M.(1973). Les ovins dans l’antiquité d’après les vestiges phéniciens et romains en Tunisie et en Algérie. INRAT, Institut National de la Recherche Agronomique de Tunisie.

Sassi-Zaidy, Y; Ben Maretto, F; Charfi-Cheikrouha, F; Cassandro, M.(2014). Genetic diversity, structure, and breed relationships in Tunisian sheep. Small Rumin Res, 1:119:52–6.

Sheriff, O; Alemayehu, K. (2018).Genetic diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs: A review. Cogent Food Agric, 4:1459062.

Tefiel, H; Ata, N; Chahbar, M; Benyarou, M; Fantazi, K; Yilmaz, O. (2018). Genetic characterization of four Algerian goat breeds assessed by microsatellite markers. Small Rumin Res, 160:65–71.

Trouette, G. (1933). La sélection ovine dans le troupeau indigène. Dir Des Serv l’Élevage Impr P Guiauchin Alger, 1–10.

Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population structure. Evolution (N Y) 1984:1358–70.

Wright , S. (1931). Evolution in Mendelian populations. Genetics, 16:97.

Yilmaz, O; Cemal, I; Karaca, O. (2014). Genetic diversity in nine native Turkish sheep breeds based on microsatellite analysis. Anim Genet, 45:604–8.

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Publicado

2024-11-22

Como Citar

Ouinten, Y., Aouadj, S. A., Medrouh, B., Lafri, I., Ranebi, D., Gaouar, S. B. S., Klenovitskii, P. M., & Benchiha, W. (2024). The genetic diversity of some North African sheep breeds using microsatellite markers. Brazilian Journal of Animal and Environmental Research, 7(4), e74802. https://doi.org/10.34188/bjaerv7n4-075

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v. 7 n. 4 (2024)

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