Clomazone herbicide impairs bioenergetics in mitochondria isolated from the thorax of honey bees (Apis mellifera L.)
DOI:
https://doi.org/10.34188/bjaerv7n2-106Palavras-chave:
herbicides, pollenizers, bioenergetics, adenosine triphosphateResumo
The excessive use of pesticides on crops has caused an increase in animal mortality, contamination of soil, water and food, in addition to being identified as one of the causes of death of bees. Despite being quite effective, the herbicide clomazone (CLZ) causes widespread environmental contamination due to its high solubility in water (1,100 mg.L-1), and its residues can remain in the environment for up to 130 days. Mitochondria are responsible for producing the majority of the ATP in cells, and in bees, the thorax, as it is where the wings are located, is the place with the greatest ATP production. The objective of this work was to evaluate the effects of the herbicide CLZ on mitochondria isolated from the thorax of honey bees (Apis mellifera). The effects of CLZ (100, 125, 150, 200 and 250 µM) on mitochondria were evaluated by determining oxygen consumption, ATP production and generation of reactive oxygen species (ROS). When energized with pyruvate + malate (complex I substrates of the respiratory chain) CLZ did not show significant results in any of the concentrations used, however, when energized with succinate (complex II substrate of the respiratory chain) a dose-dependent stimulus in mitochondrial respiration was observed. Mitochondrial ATP levels were significantly reduced in a dose-dependent manner at concentrations above 125 µM. CLZ did not significantly induce the generation of ROS at any of the concentrations studied. Thus, the results of this study indicate that CLZ toxicity in honey bees may be related to changes in mitochondrial bioenergetics.
Referências
Balieira, K.V.B., Mazzo, M., Bizerra, P.F.V., Guimarães, A.R.J. S., Nicodemo, D., Mingatto, F.E. (2018). Imidacloprid-induced oxidative stress in honey bees and the antioxidant action of caffeine. Apidologie, 49(5), p. 562–572,.
Boelsterli, U.A. (2007). Disruption of mitochondrial function and mitochondria-mediated toxicology. In: Mechanistic Toxicology: The Molecular Basis of How Chemicals Disrupt Biological Targets, 2nd edition, CRC Press, Boca Raton, p. 357-389.
Cain, K., Skilleter, D.N. (1987). Preparation and use of mitochondria in toxicological research. In: Snell, K., Mullock, B. Eds. Biochemical Toxicology. IRL, Oxford, UK, p. 217-254.
Cestonaro, L.V., da Silva, A.C.G., Garcia, S.C., Valadares, M.C., Arbo, M.D. (2024). Mitochondrial impairment related to the immunotoxicity of the herbicides clomazone, glyphosate and sulfentrazone in THP-1 cells. Toxicology Research, 13(1), tfae005.
Chance, B., Williams, G.R. (1956). The respiratory chain and oxidative phosphorylation. Advances in Enzymology and Related Subjects of Biochemistry, 17, 65-134.
Colby, S.R., Humburg, N.E. (1989). Herbicide handbook of the Weed Science Society of America. 6. ed. Champaign: The Society..
Costa-Maia, F.M., Lino-Lourenço, D.A., Toledo, V.A.A. (2010). Sistemas de Produção Agropecuária. Editora UTFPR, 1, 45-67.
Crane, E., Walker, P. (1983). The impact of pest management on bees and pollination. London: Tropical Development and Research Institute, p. 129.
Fagundes, M.Z., Gonçalves, M.A., Soares, M.P., Martins, M.L., Zanella, R., Riet-Correa, F., Anjos, B.L. (2015). Clinicopathological and toxicological aspects of poisoning by the clomazone herbicide in sheep. Small Ruminant Research, 124, 120-126.
Faita, M.R., Oliveira, E.M., Alves, V.V., Orth, A.I., Nodari, R.O. (2018). Changes in hypopharyngeal glands of nurse bees (Apis mellifera) induced by pollen-containing sublethal doses of the herbicide Roundup®. Chemosphere, 211, 566-572.
Giannini, T.C., Cordeiro, G.D., Freitas, B.M., Saraiva, A.M., Imperatriz-Fonseca, V.L. (2015). The dependence of crops for pollinators and the economic value of pollination in Brazil. Journal of Economic Entomology, 108(3), 849-857.
Goulson, D., Nicholls, E., Botías, C., Rotheray, E.L. (2015). Bee declines driven by combined stress from parasites, pesticides, and lack of flowers. Science, 347, 6229.
Guimarães, M. (2007). Colmeias às moscas: síndrome misteriosa causa sumiço de abelhas na América e na Europa. Revista Pesquisa Fapesp, 137, 50-51.
GunasekarA, A.S., Dela Cruz, I.D.P., Curtis, M.J., Claassen, V.P., Tjeerdema, R.S. (2009). The behavior of clomazone in the soil environment. Pest Management Science, 65(6), 711-716.
Hakme, E., Lozano, A., Gómez-Ramos, M.M., Hernando, M.D., Fernández-Alba, A.R. (2017). Non-target evaluation of contaminants in honey bees and pollen samples by gas chromatography time-of-flight mass spectrometry. Chemosphere, 184, 1310-1319.
Harrison, J.F., Roberts, S.P. (2000). Flight respiration and energetics. Annual Review of Physiology, 62(1), 179-205.
Hoskins, D.D., Cheldelin, V.H., Newburgh, R.W. (1956). Oxidative enzyme systems of the honey bee, Apis mellifera L. Journal of General Physiology, 39, 705-713.
IBGE, Instituto Brasileiro de Geografia e Estatística. (2022). Produção de mel de abelha. Available in: https://www.ibge.gov.br/explica/producao-agropecuaria/mel-de-abelha/br. Acessed in 30/05/2024.
Imperatriz-Fonseca, V.L., Nunes-Silva, P. (2010). As abelhas, os serviços ecossistêmicos e o Código Florestal Brasileiro. Biota Neotropica, 10, 59-62.
Khalifa, S.A.M., Elshafiey, E.H., Shetaia, A.A., El-Wahed, A.A.A., Algethami, A.F., Musharraf, S.G., AlAjmi, M.F., Zhao, C., Masry, S.H.D., Abdel-Daim, M.M., Halabi, M.F., Kai, G., Al Naggar, Y., Bishr, M., Diab, M.A.M., El-Seedi, H.R. (2021). Overview of bee pollination and its economic value for crop production. Insects, 12(8), 688.
Landim, C.C. (2009). Abelhas: morfologia e função de sistemas. São Paulo: Editora Unesp, 408p.
Lemasters, J.J., Hackenbrock, C.R. (1976). Continuous measurement and rapid kinetics of ATP synthesis in rat liver mitochondria, mitoplasts and inner membrane vesicles determined by firefly-luciferase luminescence. European Journal of Biochemistry, 67, 1-10.
Lippi, A.M.F., De Andrade, D.G., Bovo, J., Nogueira, L., Pinto, M.M. (2014). Características físico-químicas e toxicológicas do clomazona. RevInter Revista Intertox de Toxicologia, Risco Ambiental e Sociedade, 7(1), 24-39.
Malaspina, O., Silva-Zacarin, E.C.M. (2006). Cell makers for ecotoxicological studies in target organs of bees. Brazilian Journal of Morphological Sciences, 23, 303-309.
Meyer, S.A., Kulkarni, A.P. (2001). Hepatotoxicity. In: Hodgson, E., Smart, R.C. (Eds.), Introduction to Biochemical Toxicology. 3. ed. New York: John Wiley & Sons, p. 487-507.
Murphy, M.P. (2009). How mitochondria produce reactive oxygen species. Biochemical Journal, 417(1), 1-13.
Nicodemo, D., Maioli, M.A., Medeiros, H.C.D., Guelfi, M., Balieira, K.V.B., De Jong, D., Mingatto, F.E. (2014). Fipronil and imidacloprid reduce honeybee mitochondrial activity. Environmental Toxicology and Chemistry, 33(9), 2070-2075.
Nicodemo, D., Mingatto, F.E., De Jong, D., Bizerra, P.F.V., Tavares, M.A., Bellini, W.C., Vicente, E.F., Carvalho, A. (2020). Mitochondrial respiratory inhibition promoted by pyraclostrobin in fungi is also observed in honey bees. Environmental Toxicology and Chemistry, 39(6), 1267-1272.
Novais, S.M.A., Nunes, C.A., Santos, N.B., D`amico, A.R., Fernandes, G.W., Quesada, M., Braga, R.F., Neves, A.C.O. (2016). Effects of a possible pollinator crisis on food crop production in Brazil. Plos One, 11(11), e0167292.
Pham-Delègue, M.H., Decourtye, A., Kaiser, L., Devillers, J. (2002). Behavioural methods to assess the effects of pesticides on honey bees. Apidologie, 33(5), 425-432.
Ribeiro, E. (2017). Cientistas preocupados com a perda de colônias de abelhas. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Available in: https://www.embrapa.br/busca-de-noticias/-/noticia/26769725/cientistas-preocupados-com-a-perda-de-colonias-de-abelhas#:~:text=Abelhas%20no%20Brasil%20Embora%20no,e%20per%C3%ADodos%20prolongados%20de%20seca. Acessed in 30/05/2024.
Ritchie, H. (2021). “How much of the world’s food production is dependent on pollinators?” Published online at OurWorldInData.org. Available in: https://ourworldindata.org/pollinator-dependence. Accessed in 06/06/2024.
Rortais, A., Arnold, G., Halm, M., Touffet-Briens, F. (2005). Modes of honeybees exposure to systemic insecticides: estimated amounts of contaminated pollen and nectar consumed by different categories of bees. Apidologie, 36(1), 71-83.
Santos, C.F., Otesbelgue, A., Blochtein, B. (2018). The dilemma of agricultural pollination in Brazil: Beekeeping growth and insecticide use. Plos One, 13(7), 1-13.
Souza, L.D., Rodrigues, A.E., Pinto, M.S.C. (2007). As abelhas como agentes polinizadores (The bees agents pollinizer’s). Revista Eletrônica de Veterinária, 8(3), 1-7.
Souza, P.A., Rodrigues, C., Santiago, A.P.S.A., Lucas, N.C., Leitao, G.G., Galina Filho, A. (2011). Antioxidant activity of natural compounds of Stachytarpheta cayennensis by scavenger of mitochondrial reactive oxygen species. Brazilian Journal of Pharmacognosy, 21(3), 420-426.
Syromyatnikov, M.Y., Kokina, A.V., Lopatin, A.V., Starkov, A.A., Popov, V.N. (2017). Evaluation of the toxicity of fungicides to flight muscle mitochondria of bumblebee (Bombus terrestris L.). Pesticide Biochemistry and Physiology, 135, 41-46.
Syromyatnikov. M.Y., Gureev, A.P., Vitkalova, I.Y., Starkov, A.A., Popov, V.N. (2019). Unique features of flight muscles mitochondria of honey bees (Apis mellifera L.). Archives of Insect Biochemistry and Physiology, 102, e21595.
Thompson, H.M. (2001). Assessing the exposure and toxicity of pesticides to bumblebees (Bombus sp.). Apidologie, 32(4), 305-321.
Thompson, H. M. (2002). Behavioral effects of pesticides in bees- their potential for use in risk assessment. Ecotoxicology, 12(1-4), 317-330.
Van Der Steen, J.J.M. (2001). Review of the methods to determine the hazard and toxicity of pesticides to bumblebees. Apidologie, 32(5), 399-406.
Zanella, R., Primel, E.G., Machado, S.L.O., Gonçalves, F.F., Marchezan, E. (2002). Monitoring of the herbicide clomazone in environmental water samples by solid-phase extraction and high-performance liquid chromatography with ultraviolet detection. Chromatographia, 55(9-10), 573-577.
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