Bio-transference of Aflatoxin B1 from feed to the breast of hens as Aflatoxin M1

Biotransferência de Aflatoxina B1 da ração para o peito de galinhas como Aflatoxina M1

Resumo

The presence of hydroxylated metabolites of aflatoxin B1 (AFB1), such as M1 (AFM1) Aflatoxin has been reported in breast laying hen, Diaz Zaragoza et al, 2014. The hens when their laying cycle end are sent to the trail of bird for his sacrifice. In Mexico chicken consumption in the form of stock, it is much in demand mainly in the center and southeast of the country. Some companies process meat to prepare various types of sausages, such that in one way or another becomes a food for human consumption. Currently the System Product Poultry in Mexico, is working to raise awareness about food safety in the poultry industry generated as egg and meat. The AFM1 is a compound considered as a carcinogen and is limited in milk at levels of 0.5 to 0.05 µg/L. This depend the regulations of each region of the world. The given the possibility that AFM1 is a chemical risk consumption of hen breast. Evaluating the Bio-transfer this AFB1 was performed in a contaminated diet with 500 µg/kg (ppb) and quantify AFM1 contamination in the breast, after 8 days of diet consumption. At the same time included an anti-aflatoxin agent to verify their efficiency in this experiment. AFM1 concentration in the breast of the negative control group was below the detection limit of 0.001 ppb. The concentration of the positive control group was 0.004 ppb and challenge group concentration was 0.002 ppb. The analytical tests were carried out by the technique of Ultra High Performance Liquid Chromatography (UHPLC). The results show that bio-AFB1 transfer to the breast as AFM1 is minimal. Therefore, this food is considered safe. Evaluated adsorbent efficiency was 50%. Complementarily AFB1 concentration in the liver, where the toxin is quantified levels of 0.467 ppb in the positive control and 0.196 ppb in the challenge group with adsorbent was evaluated. The effectiveness of the commercial adsorbent was the 60%.