Effects of residual contamination on mechanical properties and real-time conversion of etch-and-rinse adhesive / Efeitos da contaminação residual nas propriedades mecânicas e na cinética de conversão de adesivos convencionais


  • Maria Malerba Colombi Humel
  • Lucia Trazzi Prieto
  • Josué Junior Araujo Pierote
  • Cintia Tereza Pimenta de Araujo
  • João Victor Frazão Câmara
  • Isabel Ferreira Barbosa
  • Guereth Alexsanderson Oliveira Carvalho
  • Luis Alexandre Maffei Sartini Paulillo




Chlorexidine, Dentinal adhesive, Ethanol, Mechanical properties, Water.


Introduction: An ideal hybrid layer should have water and solvents completely replaced by resin monomers, thus creating an interdiffusion zone with collagen and resin, and guaranteeing resistance and adequate mechanical properties to resist the challenges incurred in restoration. Objective: To evaluate the influence of residual contamination by different concentrations of water, ethanol and chlorexidine on mechanical properties and conversion of two etch-and-rinse adhesives. Method: Bar specimens of a model adhesive (M1) and a commercial Scotchbond MP adhesive (SBMP) were prepared and submitted to flexural strength (FS), elastic modulus (E), conversion degree (DC) and the kinetics of cure (K) evaluation. The real-time polymerization (RP) was monitored for 120 seconds. The data were statistically analyzed by two-way ANOVA and Tukey ?s test (?=0.05). Results: All solutions showed a decrease in FS and E values. For M1 adhesive, this decrease could be noted with increase of all dilutions tested. A decrease in DC values also was noted as dilutions increased, independent of solution tested. For SBMP adhesive, the mechanical properties were affected more by water and chlorexidine solutions and, it was noted an increase in DC with the increase of solution, especially for ethanol. For K test, adding water to adhesives, a progressive decrease could be noted on graphics. Conclusions: So a reduction in mechanical properties of adhesive solutions could be noted when extreme amounts of residual solutions. Nevertheless, at lowest concentrations, ethanol and chlorexidine seems not interfere on K and RP of adhesives.




- Pashley DH, Tay FR, Breschi F, Tjäderhane L, Carvalho R, Carrilho M, et al. State of the art etch?and?rinse adhesives. Dental Materials. 2011; 27:1?16.

- De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, et al. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res. 2005; 84(2): 118-32.

- Guo X, Wang Y, Spencer P, Ye Q, Yao X. Effects of water content and initiator composition on photopolymerization of a model BisGMA/HEMA resin. Dent Mater. 2008; 24(6): 824-31.

- Pucci CR, Gu LS, Zhang HY, Song Q, Xia VW, Davis LB, et al., Water-associated attributes in the contemporary dentin bonding milieu. J Dent; 2018 Apr 24. pii: S0300-5712(18)30097-6.

- Pashley DH, Tay FR, Carvalho RM, Rueggeberg FA, Agee KA, Carrilho M, et al. From dry bonding to water?wet bonding to ethanol?wet bonding. A review of the interactions between dentin matrix and solvated resins using a macromodel of the hybrid layer. Am J Dent. 2007; 20(1): 7?20.

- Ekambaram M, Yiu CK, Matinlinna JP, Chang JW, Tay FR, King NM. Effect of chlorhexidine and ethanol-wet bonding with a hydrophobic adhesive to intraradicular dentine. J Dent. 2014 Jul;42(7):872-82.

- Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjaderhane L, et al. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res. 2007; 86(6): 529-33.

- Ayar MK, A review of ethanol wet?bonding: Principles and techniques. European Journal of Dentistry Eur J Dent. 2016 Jan-Mar;10(1):155-9.

- Tja?derhane L, et al. Strategies to prevent hydrolytic degradation of the hybrid layer—A review. Dent Mater Dent Mater. 2013 Oct;29(10):999-1011

- Elliott JE, Bowman CN. Effects of solvent quality during polymerization on network structure of cross?linked methacrylate copolymers. J Phys Chem B. 2002; 106(11): 2843-2847.

- Holmes RG, Rueggeberg FA, Callan RS, Caughman F, Chan DC, Pashley DH, et al. Effect of solvent type and content on monomer conversion of a model resin system as a thin film. Dent Mater. 2007; 23(12): 1506-12.

- Pashley EL, Zhang Y, Lockwood PE, Rueggeberg FA, Pashley DH. Effects of HEMA on water evaporation from water?HEMA mixtures. Dent Mater. 1998; 14(1): 6?10.

- Stansbury JW. Dimethacrylate network formation and polymer property evolution as determined by the selection of monomers and curing conditions. Dental Materials. 2012; 28(1): 13?22.

- Ogliari FA EC, Lima GS, Conde MC, Petzhold CL, Demarco FF, Piva E. Onium salt reduces the inhibitory polymerization effect from an organic solvent in a model dental adhesive resin. J Biomed Mater Res B Appl Biomater. 2008; 86(1): 113? 118.

- Rueggeberg FA, Hashinger DT,Fairhurst CW. Calibration of FTIR conversion analysis of contemporary dental resin composites. Dent Mater. 1990; 6(4): 241? 9.

- Arrais CA, Giannini M, Rueggeberg FA. Kinetic analysis of monomer conversion in auto-and dual?polymerizing modes of commercial resin luting cements. J Prosthet Dent. 2009; 101(2): 128?36.

- Cadenaro M, Breschi L, Rueggeberg FA, Suchko M, Grodin E, Agee K, et al. Effects of residual ethanol on the rate and degree of conversion of five experimental resins. Dent Mater. 2009; 25(5): 621?8.

- Aguiar TR, Di Francescantonio M, Arrais CA, Ambrosano GM, Davanzo C,Giannini M. Influence of curing mode and time on degree of conversion of one conventional and two self-adhesive resin cements. Oper Dent. 2010; 35(3): 295-9.

- Ito S, Hashimoto M, Wadgaonkar B, Svizero N, Carvalho RM, Yiu C, et al. Effects of resin hydrophilicity on water sorption and changes in modulus of elasticity. Biomaterials. 2005; 26(33): 6449?59.

- Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials. 2007; 28(26): 3757?85.

- Ye Q, Wang Y, Williams K,Spencer P. Characterization of photopolymerization of dentin adhesives as a function of light source and irradiance. J Biomed Mater Res B Appl Biomater. 2007; 80(2): 440?6.

- Spencer P, Wang Y. Adhesive phase separation at the dentin interface under wet bonding conditions. J Biomed Mater Res. 2002; 62(3): 447?56.

- Abebe W, Maddux WF. Roles of nitric oxide and prostacyclin in triethyleneglycol dimethacrylate (TEGDMA)?induced vasorelaxation. Dent Mater. 2006. Jan; 22(1): 37-44.

- Ping ZH, Nguyen QT, Chen SM, Zhou JQ, Ding YD. States of water in different hydrophilic polymers - DSC and FTIR studies. Polymer. 2001; 42(20): 8461- 8467.

- Nogueira PRC, Torres A, Abad J, Cano J, López J, López?Bueno I, Barral L. Effect of water sorption on the structure and mechanical properties of an epoxy resin system. J Appl Polym Sci. 2001; 80: 71?80.

- Lee TY RT, Jonsson ES, Guymon CA, Hoyle CE. Influence of hydrogen bonding on photopolymerizaton rate of hydroxyalkyl acrylates. macromolecules. 2004; 37(10): 3659?3665.

- Carrilho MR, Carvalho RM, de Goes MF, di Hipolito V, Geraldeli S, Tay FR, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res. 2007; 86(1):90?4.



How to Cite

Humel, M. M. C., Prieto, L. T., Pierote, J. J. A., de Araujo, C. T. P., Câmara, J. V. F., Barbosa, I. F., Carvalho, G. A. O., & Sartini Paulillo, L. A. M. (2020). Effects of residual contamination on mechanical properties and real-time conversion of etch-and-rinse adhesive / Efeitos da contaminação residual nas propriedades mecânicas e na cinética de conversão de adesivos convencionais. Brazilian Journal of Development, 6(8), 56077–56092. https://doi.org/10.34117/bjdv6n8-137



Original Papers