Effects of ametryn on mutagenicity (Tradescantia pallida) and cell viability (Saccharomyces cerevisiae)
Keywords:
ecotoxicity, soil, herbicideAbstract
On this of uses of organic compounds, for various purposes, it is necessary to understand their effects on the environment. Thus, the use of ecotoxicological assays has helped to understand the interferences of these molecules in the test organisms. The herbicide ametryn is widely used in sugarcane crops, with great efficiency in the control of broadleaf weeds. However, it is moderately soluble in water and persistent in the environment and may remain in the ecosystem for a long time. Thus, this work aimed to evaluate the effects of ametryn on the cellular viability of Saccharomyces cerevisiae and the mutagenic effects on Tradescantia pallida. In the S. cerevisiae assays, ametryn solutions added to the yeast suspension were used. After the incubation period, live cells were quantified to calculate cell viability. For the analysis of the mutagenic effects, soil contaminated by ametryn was used as well as some treatments as a form of bioaumentation, with addition of microbial consortium and biostimulation with addition of surfactant. S. cerevisiae was less sensitive to ametryn, despite being a low cost and easy to perform assay. However, T. pallida showed to be very sensitive to the herbicide, both in the contaminated soil and only by the ametryn solution. The treatments used were effective in decreasing the frequency of micronucleus appearance. Thus, it is necessary to use multiple tools in order to assess the effects of organic compounds on the environment as well as to take mitigating measures to reduce and / or reverse the harmful effects on biota.
References
Braconi, D., Bernardini, G., Santucci, A. (2016). Saccharomyces cerevisiae as a tool to evaluate the effects of herbicides on eukaryotic life. Journal of Proteomics, 137, 19-34
Dearfield, K. L., Cimino, M. C., Mccarroll, N. E. (1998). Genotoxicity risk assessment: a proposed classification strategy. Mutation Research, 140, 234-243
Gao, G., Qian, J., Fang, D., Yu, Y., Zhi, J. (2016). Development of a mediated whole cell-based electrochemical biosensor for joint toxicity assessment of multi-pollutants using a mixed microbial consortium. Analytica Chimica Acta, 924, 21-28
Gaytán, B. D., Loguinov, A. V., Lantz, S. R., Lerot, J. M., Denslow, N. D., Vulpe, C. D. (2013). Functional Profiling Discovers the Dieldrin Organochlorinated Pesticide Affects Leucine Availability in Yeast. Toxicological Sicences, 132, 347-358
Leite, A. S., Zandonato, V. V., Fluminhan, A. (2013). Evaluation of genotoxicity caused by environmental factors in Tradescantia pallida cv purpurea through the comet assay. Electronic Journal Environmental Forum, 9, 399-417
Ma, T. H. (1981). Tradescantia micronucleus bioassay and pollen tube chromatid aberration test for in situ monitoring and mutagen screening. Environmental Health Perspectives, 37, 85-90
Ma, T. H. Tradescantia micronuclei (Trad-MN) test for environmental clastogens. (1983). In: Kolber, A. R., Wong, T. K., Grant, Lester D., De Woskin, Robert S., Hughes, J. T. In vitro toxicity testing of environmental agents. Ed. Plenum Publishing Corporation, New York, 1191-214
Mielli, A. C. (2008). Evaluation of the genotoxic activity of treated sewage sludge from the State of São Paulo with the micronucleus test in Tradescantia germ cells (Trad- MN). (Ph.D. in Sciences) - Faculty of Medicine, University of São Paulo, São Paulo.
PPDB. Pesticides properties database - Ametryn. University of Hertfordshire, 2018.
Régis, G., Bidoia, E.D. (2001). Treatment of effluent from a chemical industry through the electrolytic process aiming to reduce the toxicity monitored by the bioindicator Saccharomyces cerevisiae. Salusvita, 20, 43-51
Rodrigues, G. S., Ma, T. H., Pimentel, D., Weinstein, L. H., Ichikawa, S. Tradescantia bioassays as monitoring systems for environmental mutagenesis – a review. (1997). Critical Reviews in Plant Sciences, 16, 325-359
Rodrigues, Y. A., Christofoletti, C. A., Pedro, J., Bueno, O. C., Malaspina, O., Costa Ferreira, R. A., Fontanetti, C. S. (2015). Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid. Chemosphere, 120, 438-452.
Rumlova, L., Dolezalova, J. (2012). A new biological test utilising the yeast Saccharomyces cerevisiae for the rapid detection of toxic substances in water. Environmental Toxicology and Pharmacology, 33, 459-464
Silva, G. H., Messias, T. G., Leme, D. M., Monteiro, R. T. R. (2013). Mutagenicidade e genotoxicidade em águas superficiais e subterrâneas antes e após o tratamento da água. Holos Environment, 13, 64
Teixeira, M. C. V., Barbério, A. (2012). Biomonitoramento do ar com Tradescantia pallida (Rose) D. R. Hunt Var purpurea Boom (Commelinaceae). Revista Ambiente & Água, 7, 279-292.
