Impact of Streptococcus mutans pre-adaptation to lactose on biofilm formation in vitro
DOI:
https://doi.org/10.34119/bjhrv9n1-191Keywords:
Streptococcus mutans, biofilm, lactoseAbstract
Dental caries is a complex, polymicrobial disease related to biofilm formation. Carbohydrates in children's diets are essential for biofilm formation. The aim of this study was to compare biofilm production of Streptococcus mutans pre-adapted to lactose (PAL) and non-PAL in the presence of sucrose and/or lactose on polystyrene surfaces coated or not with saliva. S. mutans ATCC 25175 was PAL through daily subcultures in media containing 1% lactose for 96 hours. Biofilm formation by PAL and non-PAL S. mutans were performed in 96-well flat-bottom polystyrene microplates on surfaces sensitized or not with saliva, in the presence of 1% sucrose, 1% lactose, or sucrose+lactose (0.5% + 0.5%). After incubation (24 h, 36 °C, microaerophilic), the biofilm was stained with 0.2% crystal violet and quantified at 490 nm. We observed that the PAL samples formed less biofilm; the presence of sucrose, alone or with lactose, was responsible for greater biofilm production; coating with saliva reduced biofilm in the presence of lactose alone. We conclude that the greatest potential for biofilm formation was observed for the non-PAL samples in the presence of sucrose on surfaces not coated with saliva.
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