Effect of polar extracts from leaves and branches of Monteverdia quadrangulata on the Zika virus replication cycle
DOI:
https://doi.org/10.34119/bjhrv8n6-078Keywords:
Monteverdia quadrangulata, ZIKV-replication cycle, in vitro Anti-ZIKV, EC50, selectivity indexAbstract
Species of the genus Monteverdia, among them, M. quadrangulata, have been traditionally used in Brazil to treat infections and inflammation. The objective of this study was to investigate the effect of polar extracts of Monteverdia quadrangulata on viral particles and the replication cycle of Zika virus (ZIKV). The anti-ZIKV effect of ethyl acetate extracts from leaves (LAE) and branches (TAE) of M. quadrangulata was investigated in vitro, and the median effective concentration (EC50), selectivity index (SI) and mechanisms of action (virucidal activity and antiviral activity in the adsorption, internalization and post-infection stages) were evaluated. The EC50 values of the LAE and TAE extracts were ≤ 50µg/mL. LAE was the most selective (EC50 = 29 ± 9µg/mL, SI = 31) with significant virucidal activity. LAE also reduced viral load by 4 log units at different times after infection. Probable catechins and epicatechins, previously identified as constituents of LAE, act synergistically at different stages of the viral cycle, inactivating extracellular ZIKV and interfering in the viral replication cycle.
References
Almeida C, Barbieri RL, Ribeiro MV, Lopes CV, Heck RM. Espinheira-santa (Maytenus ilicifolia Mart. ex Reiss.): knowledge by herbalists and marketers in Pelotas (RS). Rev Bras Plantas Med. 2015, 17, 722-729.
Betancur-Galvis L, Jimenez-Jarava OJ, Rivas F, Mendoza-Hernández WE, González-Cardenete MA. Synergistic In Vitro Antiviral Effect of Combinations of Ivermectin, Essential Oils, and 18-(Phthalimid-2-yl)ferruginol against Arboviruses and Herpes virus. Pharmaceuticals 2023, 16, 1-18.
Bifani AM, Chan KWK, Borrenberghs D, Tan MJA, Phoo WW, Watanabe S, et al. Therapeutics for flaviviral infections. Antiviral Res. 2023, 210, 105517.
Biral L, Marcusso GM. Flora of Espírito Santo: Celastroideae (Celastraceae). Rodriguésia [Internet]. 2023; 74: e00632022.
Byler KG, Ogungbe IV, Setzer WN. In-silico screening for anti-Zika virus phytochemicals. J Mol Graph Model. 2016, 69, 78-91,.
Carneiro BM, Batista MN, Braga ACS, Nogueira ML, Rahal P. The green tea molecule EGCG inhibits Zika virus entry. Virology 2016, 496, 215-218.
Carod-Artal FJ. Neurological complications of Zika virus infection. Expert Rev Anti Infect Ther. 2018, 16, 399–410.
Chukwujekwu JC, van Staden J. In vitro Antibacterial Activity of Combretum edwardsii, Combretum krausii, and Maytenus nemorosa and Their Synergistic Effects in Combination with Antibiotics. Front Pharmacol. 2016, 31, 43–59.
Coelho C, Gallo G, Hardy L, Bottazzi ME, Campos C, Würtele M. Biochemical Screening of Potent Zika Virus Protease Inhibitors. ChemMedChem. 2022, 17, e202100695.
de Aguilar MG, Veríssimo Morais Quintão S, Camargo KC, Pains Duarte L, Vasconcelos LR, de Magalhães JC, et al. Flavonoids and Triterpenoids Isolated From the Ethyl Acetate Extract of M. quadrangulata Leaves. Chem Biodivers 2024, 21 (7):e202400636.
Dvorkin-Camiel L, Whelan JS. Tropical American plants in the treatment of infectious diseases. J Diet Suppl. 2008, 5, 349-72.
Ferraz AC, Moraes TFS, Nizer WSDC, Santos MD, Tótola AH, et al. Virucidal activity of proanthocyanidin against Mayaro virus. Antiviral Res. 2019, 168, 76-81.
Halani S, Tombindo PE, O'Reilly R, Miranda RN, Erdman LK, Whitehead C, et al. Clinical manifestations and health outcomes associated with Zika virus infections in adults: A systematic review. PLoS Negl Trop Dis. 2021, 15, e0009516.
Huang YY, Chen L, Ma GX, Xu XD, Jia XG, Deng FS, et al. A Review on Phytochemicals of the Genus Maytenus and Their Bioactive Studies. Molecules. 2021, 26, 1-41.
Knipe DM, Howley PM. Fields Virology, sex ed. Lippincott Williams & Wilkins, Philadelphia, 2013.
Kusumoto N, Morikawa T, Hashida K, Ohira T. The variability of terpenoids and flavonoids in native Lindera umbellata from the same region. J Wood Sci. 2022, 68, 1-12.
Leonhard SE, Bresani-Salvi CC, Lyra Batista JD, Cunha S, Jacobs BC, Brito Ferreira ML, et al. Guillain-Barré syndrome related to Zika virus infection: A systematic review and meta-analysis of the clinical and electrophysiological phenotype. PLoS Negl Trop Dis. 2020, 27, 14(4): e0008264.
Lillsunde KE, Festa C, Adel H, De Marino S, Lombardi V, Tilvi S, et al. Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis. Mar Drugs. 2014, 12, 4045-4068.
Lim HJ, Nguyen TT, Kim NM, Park JS, Jang TS, Kim D. Inhibitory effect of flavonoids against NS2B-NS3 protease of ZIKA virus and their structure activity relationship. Biotechnol Lett 2017, 39, 415-421.
Lowe R, Barcellos C, Brasil P, Cruz OG, Honório NA, Kuper H, et al. The Zika Virus Epidemic in Brazil: From Discovery to Future Implications. Int J Environ Res Public Health 2018, 15, 1-18.
Lyu SY, Rhim JY, Park WB. Antiherpetic activities of flavonoids against Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro. Arch Pharm Res. 2005, 28, 1293–1301.
Mariot MP, Barbieri RL. O conhecimento popular associado ao uso da espinheira-santa (Maytenus ilicifolia e M. aquifolium). Rev Bras Biociênc. 2007, 5, 666-668.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65, 55-63.
Nunes DAF, Lopes GFM, Nizer WSDC, Aguilar MG, Santos FRDS, Sousa GF, et al. Virucidal antiviral activity of Maytenus quadrangulata extract against Mayaro virus: Evidence for the presence of catechins. J Ethnopharmacol. 2023, 311, 116436.
Pereira RS, Santos FCP, Campana PRV, Costa VV, de Pádua RM, Souza DG, et al. Natural Products and Derivatives as Potential Zika virus Inhibitors: A Comprehensive Review. Viruses. Viruses 2023, 15(5):1211.
Pujol CA, Ray S, Ray B, Damonte EB. Antiviral activity against dengue virus of diverse classes of algal sulfated polysaccharides. Int J Biol Macromol 2012, 51, 412–416.
Rombi F, Bayliss R, Tuplin A, Yeoh S. The journey of Zika to the developing brain. Mol Biol Rep 2020, 47, 3097-3115.
Sangeetha K, Martín-Acebes MA, Saiz JC, Meena KS. Molecular docking and antiviral activities of plant derived compounds against zika virus. Microb Pathog. 2020, 149, 104540.
Sharma N, Murali A, Singh SK, Giri R. Epigallocatechin gallate, an active green tea compound inhibits the Zika virus entry into host cells via binding the envelope protein. Int J Biol Macromol. 2017, 104, 1046-1054.
Vázquez-Calvo Á, Jiménez de Oya N, Martín-Acebes MA, Garcia-Moruno E, Saiz JC. Antiviral Properties of the Natural Polyphenols Delphinidin and Epigallocatechin Gallate against the Flaviviruses West Nile Virus, Zika Virus, and Dengue Virus. Front Microbiol. 2017, 8, 1314.
Xu J, Xu Z, Zheng W. A Review of the Antiviral Role of Green Tea Catechins. Molecules 2017, 22, 1337.
Yadav R, Selvaraj C, Aarthy M, Kumar P, Kumar A, Singh SK, et al. Investigating into the molecular interactions of flavonoids targeting NS2B-NS3 protease from ZIKA virus through in-silico approaches. J Biomol Struct Dyn. 2021, 39, 272-284.
Yi B, Chew BXZ, Chen H, Lee RCH, Fong YD, Chin WX, et al. Antiviral Activity of Catechin against Dengue Virus Infection. Viruses 2023, 15(6):1377.
Zou M, Liu H, Li J, Yao X, Chen Y, Ke C, et al. Structure-activity relationship of flavonoid bifunctional inhibitors against Zika virus infection. Biochem Pharmacol. 2020, 177, 113962.
