Blastocystis spp. subtypes and other enteroparasites in vulnerable urban and rural communities: a prevalence study
DOI:
https://doi.org/10.34119/bjhrv8n3-171Keywords:
Blastocystis spp, subtypes, prevalence, rural communities, urban slumAbstract
Blastocystis spp. is the most common protist found in the global population, with an estimated prevalence of one billion infected individuals, primarily in developing countries. However, its role as a pathogen has been a subject of significant debate. Some studies have shown a high prevalence of this parasite among people living in socioeconomic vulnerability in certain regions of Brazil. Methodology: 96 fecal samples were collected from participants in two low-income communities in the State of Rio de Janeiro to determine the prevalence of Blastocystis spp. and its subtype distribution. Fecal samples were processed using the Hoffman Pons & Janer and Rugai techniques. Aliquots of unpreserved samples were used to identify Blastocystis spp. subtypes through PCR. Results: Gastrointestinal parasites were detected in 70% (67/96) of the samples. Blastocystis spp. was the most prevalent with a higher prevalence in urban areas (64.3%; 36/56) compared to rural areas (50%; 20/40). Endolimax nana was the second most common, with prevalence of 21.4% (12/56) in urban areas and 30% (12/40) in rural areas. Ascaris lumbricoides and Trichuris trichiura infections were exclusively found in rural areas, while Urbanorum spp. infections were detected in urban areas. Subtype analysis revealed ST1 was the most frequent in urban areas while in rural areas, ST1 and ST3 predominate. The study suggests that Blastocystis spp. was the most prevalent with a lower frequency of other intestinal parasites. Conclusions: This research highlights a possible link with socioeconomic factors, underscoring the need for improved sanitation infrastructure in these communities.
References
BARBOSA, C. V. et al. Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes. PLoS ONE, v. 13, n. 3, p. e0193860, 2018.
BELCHIOR, V. L. R. et al. Predominance of Blastocystis spp., Entamoeba histolytica/E. dispar and other protozoa evaluated through classical and molecular diagnostic techniques in two communities of Niterói-RJ/Brazil. Brazilian Journal of Health Review, v. 4, n. 3, p. 11741–11754, 2021.
BOZA, C. C. et al. Dolor abdominal crónico en un niño con Blastocystis hominis: a propósito de un caso. Revista Médica Electrónica, v. 43, n. 2, p. 1684–1824, 2021.
CABRINE-SANTOS, M. et al. Occurrence of Blastocystis spp. in Uberaba, Minas Gerais, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 3, p. 211–214, 2015.
DARWISH, B.; ABOUALCHAMAT, G.; AL NAHHAS, S. Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria. PLoS ONE, v. 18, n. 3, p. e0283291, 2023.
DEL COCO, V. F. et al. Blastocystis spp.: avances, controvérsias y desafíos futuros. Revista Argentina de Microbiología, v. 49, n. 1, p. 110–118, 2017.
DENG, L. et al. Prevalence and molecular subtyping of Blastocystis in patients with Clostridium difficile infection, Singapore. Parasites & Vectors, v. 14, p. 277–284, 2021.
DEVERA, R. A.; AMAYA, R. I. D.; BLANCO, M. Y. Y. Prevalência de parasitos intestinales em niños pré-escolares del municipio Angostura del Orinoco, estado Bolívar, Venezuela (2016-2018). Kasmera, v. 48, n. 2, p. e48231681, 2020.
EL SAFADI, D. et al. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide. BMC Infectious Diseases, v. 14, p. 164–174, 2014.
FIGUEROA, L. M.; CEDEÑO, G. D. Evaluación clínica coprológica en sujetos sintomáticos y asintomáticos con infección por Blastocystis spp. Kasmera, v. 48, n. 1, p. e48121092019, 2020.
GALINDO, L. F. et al. Pathogenicity of Blastocystis sp.: Evidence and mechanisms. Revista Cubana de Medicina Tropical, v. 66, n. 3, p. 312–321, 2014.
GALINDO, L. F.; SANTIESTEBAN, Y. A.; GÓMEZ, Y. M. Blastocistosis y urticaria, una asociación poco conocida. Revista Cubana de Higiene y Epidemiología, v. 61, p. e1501, 2024.
HOFFMAN, W. A.; PONS, J. A.; JANER, J. L. The sedimentation concentration method in schistosomiasis. Puerto Rico Journal of Public Health, v. 9, p. 281–298, 1934.
JIMÉNEZ, P.; MUÑOZ, M.; RAMIREZ, J. D. An update on the distribution of Blastocystis subtypes in the Americas. Heliyon, v. 8, n. 12, p. e12592, 2022.
KURT, O.; AL, F. D.; TANYÜKSEL, M. Eradication of Blastocystis in humans: really necessary for all? Revista de Parasitología Internacional, v. 65, p. 797–801, 2016.
LEITE, R. O.; TOMA, H. K.; ADAMI, Y. L. Diagnóstico parasitário e molecular de enteroparasitos entre crianças residentes e funcionários de uma instituição beneficente para menores no município de Niterói-RJ. Revista de Patologia Tropical, v. 43, n. 4, p. 446–458, 2014.
MELO, G. B. et al. Current status of research regarding Blastocystis sp., an enigmatic protist, in Brazil. Clinics, v. 76, p. 2489, 2021.
OLIVEIRA-ARBEX, A. P.; DAVID, E. B.; GUIMARÃES, S. Genetic diversity among children of low-income daycare center in Southeastern Brazil. Infection, Genetics and Evolution, v. 57, p. 59–63, 2018.
PINHEIRO, I. M. Avaliação comparativa de técnicas parasitológicas no diagnóstico de Blastocystis spp. e determinação de seus subtipos. 2019. Dissertação (Mestrado em Microbiologia e Parasitologia Aplicada) – Universidade Federal Fluminense, Niterói, 2019.
POLONI, J. A. T. et al. Posicionamento da Sociedade Brasileira de Análises Clínicas sobre o reporte da estrutura denominada Urbanorum spp. em amostras de fezes – 2021. Revista Brasileira de Análises Clínicas, 2021.
REBOLLA, E. M. F. et al. Children and staff members attending public urban schools in São Paulo State, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 31–38, 2016.
REIS, S. C. C. Estudo da detecção das infecções por Blastocystis spp. em indivíduos de comunidades carentes de Niterói, RJ. 2021. Dissertação (Mestrado em Saúde Materno-Infantil, Faculdade de Medicina) – Universidade Federal Fluminense, Niterói, 2021.
RUGAI, E.; MATTOS, T.; BRISOLA, A. P. A modification of Baermann's technique for isolating nematode larvae from faeces. Revista do Instituto Adolfo Lutz, v. 14, n. 1, p. 5–8, 1954.
SANTOS, B. M. et al. A study of intestinal parasites in children of an urban region with excellent sanitary condition reveals high occurrence of Blastocystis spp. Revista de Patologia Tropical, v. 46, n. 4, p. 321–330, 2017.
SCALAN, P. D. et al. Prevalence and genetic diversity of Blastocystis in family units living in the United States. Infection, Genetics and Evolution, v. 45, p. 95–97, 2016.
SKOTARCZAK, B. Genetic diversity and pathogenicity of Blastocystis. Annals of Agricultural and Environmental Medicine, v. 25, n. 3, p. 411–416, 2018.
VISCIARELLI, E. C. et al. Blastocystis: estudio coproparasitológico, clínico-epidemiológico y de prevalencia de subtipo 3 en pacientes de hospitales de Bahía Blanca, Argentina. Acta Bioquímica Clínica Latinoamericana, v. 55, n. 2, p. 195–206, 2021.
WORLD HEALTH ORGANIZATION (WHO). Guidelines for drinking-water quality. 3. ed. Geneva: WHO, 2008.
YOSHIKAWA, H. et al. Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitology Research, v. 92, p. 22–29, 2004.
ZANETTI, A. D. S. et al. Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis. Parasites & Vectors, v. 13, p. 30, 2020.
