Phytochemical components and antimicrobial properties of Thymus pallescens de Noé essential oil against nosocomial uropathogens
DOI:
https://doi.org/10.34119/bjhrv7n9-387Keywords:
Thymus pallescens de Noé, chemical composition, antimicrobial activity, nosocomial, UTI, tissemsiltAbstract
Urinary infection is a real public health problem with 15.0% of all antibiotics prescription. The aim of the present study was to investigate the in vitro antimicrobial properties of essential oil of thyme (Thymus pallescens de Noé) obtained by hydrodistillation against uropathogens isolated from urine samples of patients placed in the Hospital of Thniet El had. Chromatographic analysis showed that the major compounds of T. pallescens de Noé oil were respectively carvacrol (69.85%) and ɣ-terpinene (11.44%). The essential oil of Thymus pallescens de Noé revealed significant antimicrobial activity. The highest antimicrobial activity appeared to be against Candida albicans with a mean zone of inhibition diameter of 46.33±0.57mm. Thyme had the highestt zones of inhibition against Gram-positive bacteria S saprophyticus and Micrococcus sp than Gram-negative bacteria: Klebsella pneumonia, Escherichia coli and Enterobacter aerogens with a mean halo of inhibition diameter of 27.33±1.15, 42.66±2.08, 21.33±0.57, 21.66±2.08 and 18.66±0.57 mm respectively. In conclusion, Thymus pallescens de Noé possesses a great potential as antiseptic related to its major component carvacole and can be used for the the treatment of UTI.
References
Adams R Identification of essential oil components by gas chromatography/mass spectrometry: Allured Publishing Corporation, Carol Stream 2007
Andrea Barra Factors affecting chemical variability of essential oils: a review of recent developments Nat Prod Commun. 2009 Aug;4(8):1147-54.
Atif ML., Bezzaoucha A., Mesbah S., Djellato S., Boubechou N., Bellouni R. Evolution of nosocomial infection prevalence in an Algeria university hospital (2001 to 2005). Med Mal Infect . 2006;36:423–8. doi: 10.1016/j.medmal.2006.05.002
Babushok, V., Linstrom, P., Zenkevich, I Retention Indices for Frequently Reported Compounds of Plant Essential Oils J PhysChem Ref Data, 40(4), 43101-43148. http://dxdoiorg/101063/13653552 . 2011
Benchabane, O., Hazzit, M., Mouhouche, F. et al. Influence of Extraction Duration on the Chemical Composition and Biological Activities of Essential Oil of Thymus pallescens de Noé. Arab J Sci Eng 40, 1855–1865 (2015). https://doi.org/10.1007/s13369-015-1694-x
Bendahou, M., Muselli, A., Grignon-Dubois, M., Benyoucef, M., Desjobert, J.M., Bernardini, A.F. and Costa, J. 2008. Antimicrobial activity and chemical composition of Origanum glandulosum Desf. Essential oil and extract obtained by microwave extraction: Comparison with hydrodistillation. Food Chemistry 106:132-139.
Bermingham SL., Ashe JF. Systematic review of the impact of urinary tract infections on health-related quality of life. BJU Int. 2012 Dec; 110(11 Pt C): E830-E836.
Chenoweth CE., Gould CV., Saint S. Diagnosis, management, and prevention of catheter-associated urinary tract infections. Infectious Disease Clinics of North America. 2014;28:105-119
Das, S. Natural therapeutics for urinary tract infections—A review. Future J. Pharm. Sci. 2020, 6, 64.
Gardeli, C., Papageorgiou, V., Mallouchos, A., Theodosis, K. and Komaitis, M. 2008. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L: Evaluation of antioxidant capacity of methanolic extracts. Food Chemistry 107: 1120-1130.
Gedikoğlu, S. Münevver, Ç. Ayşe Evaluation of Thymus vulgaris and Thymbra spicata essential oils and plant extracts for chemical composition, antioxidant, and antimicrobial properties Food Sci. Nutrit., 7 (5) (2019), pp. 1704-1714
Hazzit, M.; Baaliouamer, A.; Veríssimo, A. R.; Faleiro, M. L.; Miguel, M. G. Chemical composition and biological activities of Algerian Thymus oils Food Chem. 2009, 116, 714–721.
Henni Asma., Mohamed Chahbar., Hakim Tefiel., Ahmed Touahri., Kada Mohamed Amine Chouhim Study of the chemical composition of the essential oil derived from Thymus lancéolatus desf, an endemic aromatic plant native from Algeria and evaluation of its efficacy against Paenibacillus larvae and Ascosphaera Apis, two diseases affecting the honeybees, Apis mellifera DOI: https://doi.org/10.34188/bjaerv7n4-003 Brazilian Journal of Animal and Environmental ResearchISSN: 2595-573 2024
Hidron AI., Edwards JR., Patel J. NHSN annual update: antimicrobial-resistant pathogens sociated with healthcare-associated infections: as annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention,2006 -2007. Infect Control Hosp Epidemiol. 2008;29(11):996–1011. doi: 10.1086/591861.
Hudaib Mohammad ., Ester Speroni , Anna Maria Di Pietra ., Vanni Cavrini GC/MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle Journal of Pharmaceutical and Biomedical Analysis Volume 29, Issue 4, 20 July 2002, Pages 691-700
Humphtrys H., R.G. Newcombe J.E., Enstone Smith E T G. McIlvenny Four Country Healthcare Associated Infection Prevalence Survey 2006: Risk factor analysis. The journal of hospital infection 2008 . 69 (3) 249-257
Jan E. Patterson ., Leticia McElmeel., and Nathan P. Wiederhold In Vitro Activity of Essential Oils Against GramPositive and Gram-Negative Clinical Isolates, Including Carbapenem-Resistant Enterobacteriaceae Open Forum Infectious Diseases, Volume 6, Issue 12, December 2019, ofz502, https://doi.org/10.1093/ofid/ofz502
Khan I., Bahuguna A., Shukla S., Aziz F., Chauhan A.K., Ansari M.B., Bajpai V.K., Huh b. Pathog. 2020;142:104046. doi: 10.1016/j.micpath.2020.104046.
Lazrak Mohamed Amine., Ghita El Bardai1., Soumia Jaafour., Nadia Kabbali., Mohamed Arrayhani., Tarik Sqalli Houssaini Profil de l’infection urinaire nosocomiale dans un service de nephrology Pan Afr Med J . 2014 Sep 23;19:59. doi: 10.11604/pamj.2014.19.59.4835
Mahmood, M.S.; Ashraf, A.; Ali, S.; Siddique, A.B.; Asad, F.; Abbas, R.Z.; Rafique, A. Portrayal of Punica granatum L. peel extract through high performance liquid chromatography and antimicrobial activity evaluation. Braz. J. Biol. 2021, 83, e244435.
Mainul Haque, Massimo Sartelli, Judy McKimm, Muhamad Abu Bakar Health care-associated infections – an overview Infection and drug resistance 2018: 11 2321-2333
Marinelli L., Di Stefano A., Cacciatore I. Carvacrol and Its Derivatives as Antibacterial Agents. Phytochem. Rev. 2018;17:903–921. doi: 10.1007/s11101-018-9569-x.
Miloš Nikolić., Jasmina Glamočlija., IsabelC.F.R. Ferreira ., Angela Fernandes., Tatjana Marković c., Marina Soković Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils Industrial Crops and Products Volume 52, January 2014, Pages 183-190
Nabet Nacim Boudries, H., Loupassaki, S., Souagui, S., Madani, K. and Carbonell-Barrachina, Á.A. Chemical composition, antimicrobial and antioxidant activities of Thymus fontanesii Boiss. et Reut. and Origanum glandulosum Desf. essential oils International Food Research Journal 24(6): 2518-2525 (December 2017)
Nagoor Meeran, M.F.; Javed, H.; Al Taee, H. Pharmacological Properties and Molecular Mechanisms of Thymol: Prospects for Its Therapeutic Potential and Pharmaceutical Development. Front. Pharmacol. 2017, 8, 380.
Parlak E, Erol S, Kizilkaya M, Altoparlak U, Parlak M. [Nosocomial urinary tract infections in the intensive care unit patients]. Mikrobiyol Bul. 2007;41(1):39–49. [PubMed ID: 17427551].
Rana MN, Masood MK, Fahim U,Siddiqui AS. Effectiveness of herbal combination of Ivy, Thyme and Cisti extracts in management of acute cough in children and adults. Khyber Med Univ J 2019;11(3):165-9. DOI: 10.35845/kmuj.2019.1888
Sahena Ferdosh 2023 Ethnobotanical Review of Selected Medicinal Plants in Guam for the Treatment of Urinary Tract Ailments and Their Pharmacological Properties Sci. Pharm. 2023, 91(3), 43; https://doi.org/10.3390/scipharm91030043
Sönmez, A.Y., Bilen, S., Alak, G., Hisar, O., Yanık, T., Biswas, G., 2015. Growth performance and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss) juveniles fed diets
Szczepanik M., B. Zawitowska and A. Szumny Insecticidal activities of Thymus vulgaris essential oil and its components (thymol and carvacrol) against larvae of lesser mealworm, Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae) Allelopathy Journal 30 (1): 129-142 (2012)
Tache, A.M.; Dinu, L.D.; Vamanu, E. Novel insights on plant extracts to prevent and treat recurrent urinary tract infections. Appl.Sci. 2022, 12, 2635.
Wiegand I, Hilpert K, Hancock REW. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163–75.
