Efeito antibacteriano do cardol sobre bactérias orais associadas à cárie dentária e à periodontite apical crônica
DOI:
https://doi.org/10.34119/bjhrv6n3-269Keywords:
biofilme, cárie dentária, periodontite apical, Anacardium ocidentale, agente antimicrobianoAbstract
O objetivo do presente trabalho foi avaliar, in vitro, o potencial antibacteriano do cardol, um derivado do LCC, sobre bactérias orais associadas a cárie dentária e a periodontite apical crônica. Para isso, os ensaios foram realizados em cultura de células planctônicas e em biofilme, utilizando as cepas Streptococcus mutans UA159, Streptococcus parasanguinis ATCC903 e Enterococcus faecalis ATCC19433, ajustadas na concentração de 2 x 106 μg/mL. Inicialmente, as cepas foram submetidas à técnica de microdiluição seriada na presença de cardol em diferentes concentrações (0.78 a 50 μg/mL) para determinar a concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM). A avaliação da atividade antibiofilme do cardol foi realizada através dos testes de quantificação de biomassa (teste colorimétrico com cristal violeta 1%) e enumeração de células viáveis do biofilme (contagem de unidades formadoras de colônias). Os resultados foram analisados por one-way ANOVA e pós-teste de Bonferroni (p<0,05). Em relação ao efeito sobre células planctônicas, o cardol mostrou forte atividade inibitória e bactericida contra todas as cepas analisadas no estudo, com valores de CIM em 12,5 a 25 μg/mL e CBM em 25 a 50 μg/mL, respectivamente. No ensaio em biofilme, o cardol reduziu a biomassa bacteriana em todas as concentrações testadas, sendo responsável por uma redução de 96% da biomassa de E. faecalis na concentração de 1,56 μg.mL Ademais, o número de células viáveis do biofilme reduziu 100% nas maiores concentrações testadas (3,12 - 50 μg/mL) contra todas as cepas. Portanto, o cardol possui potencial terapêutico contra bactérias associadas as patologias orais.
References
ALBAYRAK, S. et al. Antioxidant and antimicrobial activities of different extracts of some medicinal herbs consumed as tea and spices in turkey. Journal of Food Biochemistry, v. 36, n. 5, p. 547–554, 2012.
ALVES, M. J. et al. Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology, v. 115, n. 2, p. 346–357, 2013.
ANAND, G. et al. In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care. Journal of Pharmacy and Bioallied Sciences, v. 7, n. 1, p. 69–74, 2015.
AZIZAN, N. et al. Composition and antibacterial activity of the essential oils of orthosiphon stamineus benth and ficus deltoidea jack against pathogenic oral bacteria. Molecules, v. 22, n. 12, 2017.
CLINICAL AND LABORATORY STANDARDS INSTITUTE. M07-A10 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Tenth Edition. Disponível em: . Acessado em: 28 de maio de 2022.
CUSHNIE, T. P. T.; LAMB, A. J. Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, v. 38 p. 99-107, 2011.
DA SILVA, B. R. et al. Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms. Biofouling, v. 33, n. 10, p. 835–846, 2017.
DAGLI, N. et al. Essential oils, their therapeutic properties, and implication in dentistry: A review. Journal of International Society of Preventive and Community Dentistry, v. 5, n. 5, p. 335-340, 2015.
DE ARAÚJO, J. S. C. et al. Antibacterial activity against cariogenic bacteria and cytotoxic and genotoxic potential of Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Brenan extracts. Archives of Oral Biology, v. 85, p. 113–119, 2018.
FEJERSKOV, O. Communify Dentistry and Oral Epidemiology Concepts of dental caries and their consequences for understanding the disease. Conmiwiity Dent Oral Epidemiol, v. 25, p. 5–12, 1997.
FLEMMING, H. C. et al. Biofilms: An emergent form of bacterial lifeNature Reviews Microbiology. Nature Publishing Group, v. 14, p. 563-575, 2016.
FREIRES, I. A. et al. Antibacterial Activity of Essential Oils and Their Isolated
Constituents against Cariogenic Bacteria: A Systematic Review. Molecules, v. 20, p. 7329-7358, 2015.
FUGOLIN, A. P. P.; PFEIFER, C. S. New Resins for Dental Composites. Journal of Dental Research, v. 96, n. 10, p. 85-91, 2017.
FURTADO, M. A. M. et al. Effect of cashew (Anacardium occidentale L.) peduncle bagasse extract on Streptococcus mutans and its biofilm. R. bras. Bioci, v. 12, n. 1, p. 9-13, 2014.
GRÖNROOS, L.; ALALUUSUA, S. Site-Specific Oral Colonization of Mutans Streptococci Detected by Arbitrarily Primed PCR Fingerprinting. Caries Research, v. 34, p. 474-480, 2000.
JHAJHARIA, K. et al. Biofilm in endodontics: A reviw. Journal of International Society of Preventive and Community Dentistry, v. 5, n. 1, p. 1-12, 2015.
KAMATH, V.; RAJINI, P. S. The efficacy of cashew nut (Anacardium occidentale L.) skin extract as a free radical scavenger. Food Chemistry, v. 103, n. 2, p. 428–433, 2007.
KASSEBAUM, N. J. et al. Global burden of untreated caries: A systematic review and metaregression. Journal of Dental Research, v. 94, n. 5, p. 650–658, 2015.
KHALIFA, L. et al. Targeting Enterococcus faecalis biofilms with phage therapy. Applied and Environmental Microbiology, v. 81, n. 8, p. 2696–2705, 2015.
KLEIN, M. I. et al. Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms. Frontiers in Cellular and Infection Microbiology, v. 5, n. 10 , p. 1-8, 2015.
KOLENBRANDER, P. E. et al. Oral multispecies biofilm development and the key role of cell-cell distance. Nature Reviews Microbiology, v. 8, p. 471-480, 2010.
KUBO, I. et al. Structure-Antibacterial Activity Relationships of Anacardic AcidsJ. Agric. Food Chem, v.41, n.6, p.1016-1019, 1993.
LI, A. N. et al. Resources and biological activities of natural polyphenols. Nutrients, v. 6, p. 6020-6047, 2014.
LIMA, C. A. DE A.; PASTORE, G. M.; LIMA, E. D. P. DE A. Estudo da atividade antimicrobiana dos ácidos anacárdicos do óleo da casca da castanha de caju (CNSL) dos clones de cajueiro-anão-precoce CCP-76 e CCP-09 em cinco estágios de maturação sobre microrganismos da cavidade bucal. Ciência e Tecnologia de Alimentos, v. 20, n. 3, p. 358–362, 2000.
LIMOLI, D. H.; JONES, C. J.; WOZNIAK, D. J. Bacterial Extracellular Polysaccharides in Biofilm Formation and Function. Microbiology Spectrum, v. 3, n. 3, p. 1–30, 2015.
LOMONACO, D.; MAIA, F.J.N.; MAZZETO, S.E. Thermal evaluation of cashew nutshell liquid as new bioadditives for poly(methyl methacrylate). J. Therm Anal Calorim. v. 111, p. 619–626, 2013.
LOMONACO, D.; MELE, G.; MAZZETTO, S. E. Cashew nutshell liquid (CNSL): From an agro-industrial waste to a sustainable alternative to petrochemical resources. Cashew Nut Shell Liquid: A Goldfield for Functional Materials (p. 19-38). Online. Springer International Publishing, 2017. E-book. Disponível em: < https://doi.org/10.1007/978-3-319-47455-7_2>. Acessado em: 28 de maio de 2022.
MACHIULSKIENE, V. et al. Terminology of Dental Caries and Dental Caries Management: Consensus Report of a Workshop Organized by ORCA and Cariology Research Group of IADR. Caries Research, v.54, p. 7-14, 2020.
MAZZETTO, S. E; LOMONACO, D.; MELE, G. Oleo Da Castanha De Caju:
Oportunidades E Desafios No Contexto Do Desenvolvimento E Sustentabilidade
Industrial. Quím. Nova, v. 32, n. 3, p. 732–741. 2009.
MELO CAVALCANTE, A. A. et al. Mutagenicity, antioxidant potential, and antimutagenic activity against hydrogen peroxide of cashew (Anacardium occidentale) apple juice and cajuina. Environmental and Molecular Mutagenesis, v. 41, n. 5, p. 360–369, 2003.
MOREIRA, M. A. et al. Efficacy of new natural biomodification agents from
Anacardiaceae extracts on dentin collagen cross-linkings. Dental Materials, v. 33, n. 10, p. 1103-1109, 2017.
OH, S. et al. Potency of cashew nut shell liquid in rumen modulation under different dietary conditions and indication of its surfactant action against rumen bacteria. Journal of Animal Science and Technology. v. 59, n. 1, p. 1-7, 2017.
PARISOTTO, T. M. et al. Relationship among microbiological composition and presence of dental plaque, sugar exposure, social factors and different stages of early childhood caries. Archives of Oral Biology, v. 55, n. 5, p. 365–373, 2010.
PINHEIRO, E. T.; MAYER, M. P. A. Enterococcus faecalis in Oral Infections. J Interdiscipl Med Dent Sci, v. 3, n. 1, p. 1-5, 2014
PITTS, N. B. et al. Dental caries. Nature Reviews Disease Primers, v. 3, n. 17030, p. 1-16, 2017.
RAHIMI, S. et al. A Review of Antibacterial Agents in Endodontic Treatment. Iranian Endodontic Journal, v. 9, n. 3, p. 161-168, 2014.
SEMEDO, T. et al. Comparative Study Using Type Strains and Clinical and Food Isolates To Examine Hemolytic Activity and Occurrence of the cyl Operon in Enterococci. J. Clin. Microbiol, v. 41, n. 6, p. 2569-2576, 2003.
SLOBODNÍKOVÁ, L. et al. Antibiofilm activity of plant polyphenols. Molecules, v. 21, n. 1717, p. 1-15, 2016.
SMITH, A. H.; ZOETENDAL, E.; MACKIE, R. I. Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microbial Ecology, v. 50, p. 197-205, 2005.
SOUZA, N. O. et al. Cashew nut shell liquids: Antimicrobial compounds in prevention and control of the oral biofilms. Archives of Oral Biology, v. 133, p. 1-10, 2022.
STASIUK, M.; KOZUBEK, A. Biological activity of phenolic lipids. Cellular and Molecular Life Sciences, v. 67, p. 841-860, 2010.
TREVISAN, M. T. S. et al. Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity. Food and Chemical Toxicology, v. 44, n. 2, p. 188–197, 2006.
VIZZOTTO, M.; KROLOW, A. C.; WEBER, G.E.B. Metabólitos Secundários Encontrados em Plantas e sua Importância. Pelotas: Embrapa Clima Temperado, 2010. 16 p. – Embrapa Clima Temperado. Documentos, 316. 2010. ISSN 1516-8840.
WANG, H. et al. Novel dental composite with capability to suppress cariogenic species and promote non-cariogenic species in oral biofilms. Materials Science and Engineering C, v. 94, p. 587–596, 2019.
WANG, Q. Q. et al. Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis. International Journal of Oral Science, v. 4, n. 1, p. 19–23, 2012.
ZERO, D. T. et al. The Biology, Prevention, Diagnosis and Treatment of Dental Caries. The Journal of the American Dental Association, v. 140, p. 25-34, 2009.
ZHANG, C.; DU, J.; PENG, Z. Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review. Journal of Endodontics, v. 41, n. 8, p. 1207-1213, 2015.
