Electrophoretic profile of cell wall extracts from Candida albicans samples isolated from women with vulvovaginitis / Perfil eletroforético de extratos da parede celular de amostras de Candida albicans isoladas de mulheres com vulvovaginite
DOI:
https://doi.org/10.34119/bjhrv5n2-214Keywords:
protein, coca liquid, sds-page.Abstract
Candida albicans is a common commensal fungus in the human microbiota. It causes not only opportunistic infections, as in vulvovaginal candidiasis, but also allergic reactions in people sensitized to the fungus. The present study evaluated the ability of Coca liquid to extract glycoproteins considered fungal antigens from Candida albicans samples, with protein bands being visualized by SDS-PAGE electrophoresis. Five strains of Candida albicans were used, which were subjected to extraction with Coca liquid (0.28% NAHCO3, 0.49% NaCl), protein and carbohydrate were dosed in the supernatant of the extract and subsequently submitted to electrophoresis. We concluded that the Coca Liquid had great capacity for protein extraction, in addition to being a simple and inexpensive method.
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