Morphometric analysis of liver and spleen type I and III collagen of autopsied patients with acquired immunodeficiency syndrome / Análise morfométrica do colágeno tipo I e III do fígado e baço de pacientes autopsiados com a Síndrome da Imunodeficiência Adquirida
DOI:
https://doi.org/10.34119/bjhrv2n5-062Keywords:
AIDS, autopsy, spleen, liver.Abstract
Acquired Immunodeficiency Syndrome (AIDS) is caused by the Human Immunodeficiency Virus (HIV), a retrovirus capable of invading and replicating in host leucocytes, favoring installation of opportunistic infections and causing important changes in organs such as spleen and liver. The aim of this study was to quantify percentage of type I and III collagen in the spleen and liver of autopsied patients divided in two groups: with AIDS (n=24) and without AIDS (n=24). Quantification of type I and type III collagen was made with Sirius Red and Reticulin stains, respectively, using the image analysis system Leica Qwin Plus®15. From medical records, we retrieved information regarding age, sex, race, Body Mass Index, and spleen and liver weight. We analyzed the data with SigmaStat® 2.03, considering statistically significant values of p<0.05. Patients with AIDS showed higher percentage of type I and type III collagen, with significant difference only between type III in both organs. The virus contributes with increase the amount of type I and type III collagen in liver and spleen of HIV infected patients contributing to morphofunctional changes. These changes may be related to decline of imune function due to viral presence and the increase of these fibers in those organs.
References
VEAZEY, R.S.; MARX, P.A.; LACKNER, A. A. The mucosal immune system: Primary targets for HIV and AIDS. Trends Immunol, v. 22: n.11, p.626–633. 2001.
BRASIL. Ministério da Saúde. Boletim epidemiológico HIV-AIDS. Ano V, Brasília: Secretaria de Vigilância em Saúde; 2016. file:///C:/boletim_2016_1_pdf_16375.pdf Acesso em 16/09/2019.
BOASSO, A.; VACCARI, M.; HRYNIEWICZ, A.; FUCHS, D.; NACSA, J.; CECCHINATO, V.; ANDERSSON, J.; FRANCHINI, G.; SHEARER, G.M.; CHOUGNET, C. Regulatory t-cell markers, indoleamine 2,3-dioxygenase, and virus levels in spleen and gut during progressive simian immunodeficiency virus infection. J Virol, v.81, n.21, p.11593-11603. 2007.
ABBAS, A.K.; LICHTMAN, A.J.; PILLAI, S. Imunologia celular e molecular. 8 ed. Elsevier. Rio de Janeiro: 2015.
ZENG, M.; SOUTHERN, P.J.; REILL,Y. C. S.; BEILMAN, G. J.; CHIPMAN, J.G.; SCHACKER, T.W.; HAASE, A. T. Lymphoid tissue damage in HIV-1 infection depletes naive T cells and limits T Cell reconstitution after antiretroviral therapy. PLoS Pathog. v.8, n.1, p.1-13. 2012.
BEGHINI, M.; MONTES, J. M.; RODRIGUES, D.B.; TEIXEIRA, V.P.; PEREIRA, S.A. Reduction of immunity in HIV-infected individuals: can fibrosis induce hypoplasia in palatine and lingual tonsils of individuals with HIV infection?. Pathol Res Pract. v. 211, n.1, p. 27-35. 2015.
JUNQUEIRA, L.C.U.; CARNEIRO, J. Histologia Básica. Textos e Atlas. 13 ed. Editora: Guanabara Koogan. Rio de Janeiro, 2017.
MACÍAS, J.; REAL, L.M.; RIVERO-JUÁREZ, A.; MERCHANTE, N.; CAMACHO, A.; NEUKAM, K.; RIVERO, A., MANCEBO, M.; PINEDA, J.A. Changes in liver steatosis evaluated by transient elastography with the controlled attenuation parameter in HIV-infected patients. HIV Med. v. 17, n.10, p.766-73. 2016.
SWANSON, S.; MA, Y.; SCHERZER, R.; HUHN, G.; FRENCH, A.L.; PLANKEY, M.W.; GRUNFELD, C.; ROSENBERG, W.M.; PETERS, M.G.; TIEN, P.C. Association of HIV, hepatitis C virus, and liver fibrosis severity with the enhanced liver fibrosis score. J Infect Dis, v.213, n.7, p. 1079-1086. 2016.
BARBOSA, R.M.R.; FORNÉS, N.S. Avaliação nutricional em pacientes infectados pelo Vírus da Imunodeficiência Adquirida. Rev. Nutr, v. 16, n. 4, p.461-470. 2003.
BRUNO, R.; GALASTR,I S.; SACCHI, P.; CIMA, S.; CALIGIURI, A.; DEFRANCO, R.; MILANI, S.; GESSANI, S.; FANTUZZI, L.; LIOTA, F.; FROSALI, F.; ANTONNUCI, G.; PINZANI, M.; MARRA.; F. gp120 modulates the biology of human hepatic stellate cells: a link between HIV infection and liver fibrogenesis. Gut, v.59, n.4, p.513?520. 2010.
TUYAMA, A.C.; HONG, F.; SAIMAN, Y.; WANG, C.; OZKOK, D.; MOSOIAN, A.; CHEN, P.; CHEN, B.K.; KLOTMAN, M.E.; BANSAL, M.B. Human immunode?ciency virus (HIV)-1 infects human hepatic stellate cells and promotes collagen 1 and monocyte chemoattractant protein-1 expression: implications for the pathogenesis of HIV/hepatitis C virus-induced liver ?brosis. Hepatology, v.52, n.2, p. 612-622. 2010.
SCHACKER, T.W.; NGUYEN, P.L.; BEILMAN, G.J.; WOLINSKY, S.; LARSON, M.; REILLY, C.; HAASE, A.T. Collagen deposition in HIV-1 infected lymphatic tissues and T cell homeostasis. J Clin Invest, 110, n.8, p. 1133-39. 2002.
SCHACKER, T.W.; BRENCHLEY, J.M.; BEILMAN, G.J.; REILLY, C.; PAMBUCCIAN, S.E.; TAYLOR, J.; SKARD, D.; LARSON, M.; DOUEK, D.C.; HAASE, A.T. Lymphatic tissue fibrosis is associated with reduced numbers of naive CD4+ T cells in human immunode?ciency virus type 1 infection. Clin Vaccine Immunol, v.13, n.5, p.556-60. 2006.
SANCHEZ, J.L.; HUNT, P.W.; REILLY, C.S.; HATANO, H.; BEILMAN, G.J.; KHORUTS, A.; JASURDA, J.S.; SOMSOUK, M.; THORKELSON, A.; RUSS, S.; ANDERSON, J.; DEEKS, S.G.; SCHACKER, T.W. Lymphoid fibrosis occurs in long-term nonprogressors and persists with antiretroviral therapy but may be reversible with curative interventions. J Infect Dis, v.211, n.7, p.1068-1075. 2011.
ANADOL, E.; LUST, K.; BOESECKE, C.; SCHWARZE-ZANDER, C.; MOHR, R.; WASMUTH, J.C.; ROCKSTROH, J.K.; TREBICKA, J. Exposure to previous cART is associated with significant liver fibrosis and cirrhosis in human immunodeficiency virus-infected patient. PLoS One, v.13, n.1p.1-12. 2018.
OLEGARIO JG, SILVA RC, TEIXEIRA VDE P, CASTRO EC, CORRÊA RR. Mucosa-associated lymphoid tissue in individuals with AIDS. Braz J Otorhinolaryngol, v.77, n.3, p. 308-314. 2011.
